Parylene peel-off arrays to probe the role of cell–cell interactions in tumour angiogenesis

Tan, Christine; Seo, Bo Ri; Brooks, Daniel J.; Chandler, Emily M.; Craighead, Harold G.; Fischbach, Claudia

doi:10.1039/b908036h

Cited by 54 publications

(52 citation statements)

References 46 publications

(49 reference statements)

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“…For example, O 2 -dependent changes in cell proliferation may alter cell-cell interactions via direct cell-cell contact and altered paracrine signaling. 38,39 By covalent coupling of cell-cell adhesion molecules (e.g., cadherins) to alginate or utilization of alginate-based matrices to recreate biologically inspired delivery profiles of soluble factors (e.g., gradients), it may become possible to study the role of these cues under defined O 2 and 3D culture conditions. Finally, while ambient O 2 is commonly employed in cell culture work, physiological tissue O 2 levels are significantly lower.…”

Section: Verbridge Et Almentioning

confidence: 99%

Oxygen-Controlled Three-Dimensional Cultures to Analyze Tumor Angiogenesis

Verbridge

Choi

Zheng

et al. 2010

Tissue Engineering Part A

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Tumor angiogenesis is controlled by the integrated action of physicochemical and biological cues; however, the individual contributions of these cues are not well understood. We have designed alginate-based microscale tumor models to define the distinct importance of oxygen concentration, culture dimensionality, and cellextracellular matrix interactions on the angiogenic capability of oral squamous cell carcinoma, and have verified the relevance of our findings with U87 glioblastoma cells. Our results revealed qualitative differences in the microenvironmental regulation of vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) secretion in three-dimensional (3D) culture. Specifically, IL-8 secretion was highest under ambient conditions, whereas VEGF secretion was highest in hypoxic cultures. Additionally, 3D integrin engagement by RGD-modified alginate matrices increased IL-8 secretion independently of oxygen, whereas VEGF secretion was only moderately affected by cell-extracellular matrix interactions. Using two-dimensional migration assays and a new 3D tumor angiogenesis model, we demonstrated that the resulting angiogenic signaling promotes tumor angiogenesis by increasing endothelial cell migration and invasion. Collectively, tissue-engineered tumor models improve our understanding of tumor angiogenesis, which may ultimately advance anticancer therapies.

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Section: Verbridge Et Almentioning

confidence: 99%

Oxygen-Controlled Three-Dimensional Cultures to Analyze Tumor Angiogenesis

Verbridge

Choi

Zheng

et al. 2010

Tissue Engineering Part A

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“…Several approaches have been designed for this purpose, e.g., inkjet cell printing (3)(4)(5)(6), surface engineering (7)(8)(9)(10)(11)(12)(13)(14)(15), and physical constraints (16)(17)(18)(19)(20)(21)(22)(23). However, finding a method that completely satisfies the above requirements remains a challenge.…”

mentioning

confidence: 99%

Block-Cell-Printing for live single-cell printing

Zhang¹,

Chou

Xia

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

127

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A unique live-cell printing technique, termed "Block-Cell-Printing" (BloC-Printing), allows for convenient, precise, multiplexed, and high-throughput printing of functional single-cell arrays. Adapted from woodblock printing techniques, the approach employs microfluidic arrays of hook-shaped traps to hold cells at designated positions and directly transfer the anchored cells onto various substrates. BloC-Printing has a minimum turnaround time of 0.5 h, a maximum resolution of 5 μm, close to 100% cell viability, the ability to handle multiple cell types, and efficiently construct protrusion-connected single-cell arrays. The approach enables the large-scale formation of heterotypic cell pairs with controlled morphology and allows for material transport through gap junction intercellular communication. When six types of breast cancer cells are allowed to extend membrane protrusions in the BloC-Printing device for 3 h, multiple biophysical characteristics of cells-including the protrusion percentage, extension rate, and cell length-are easily quantified and found to correlate well with their migration levels. In light of this discovery, BloC-Printing may serve as a rapid and high-throughput cell protrusion characterization tool to measure the invasion and migration capability of cancer cells. Furthermore, primary neurons are also compatible with BloC-Printing.cell array | cell communication | protrusion profiling | neuron patterning C urrent high-throughput screening of cell function and heterogeneity and in vitro cell-cell communication studies requires routine generation of large-scale single-cell arrays with high precision and efficiency, single-cell resolution, multiple cell types, and maintenance of cell viability and function (1, 2). Several approaches have been designed for this purpose, e.g., inkjet cell printing (3-6), surface engineering (7-15), and physical constraints (16-23). However, finding a method that completely satisfies the above requirements remains a challenge. Potentially useful and convenient tools may be available by adapting traditional printing tools to cell printing. In particular, woodblock printing is an efficient and convenient technology that revolutionized the printing world more than 1,800 y ago and was extended to microcontact molecular printing ∼20 y ago (24). However, application of the block-printing concept to cells has never been achieved. The main challenges are (i) inking cells to their molds with precision and maintaining viability, (ii) evenly and gently applying and transferring the cells to a substrate and successfully lifting off the mold without detaching the cells, and (iii) maintaining cell functions after printing.We report here the development and testing of a technology called "Block-Cell-Printing" (BloC-Printing), which involves directly inking cells to a predesigned mold and then transferring the cells to a substrate. We overcome the challenges described above by flow patterning, instead of pressing, the ink objects, as in woodblock or microcontact printing. By ...

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“…26 However, during tumorigenesis these transmembrane proteins are often downregulated, 27,28 which can induce a change in cell-cell interactions as well as impair tissue polarity, both of which can regulate the angiogenic capability of tumor cells. 29,30 Altered microenvironmental concentrations of pro-angiogenic factors, in turn, control the formation of intercellular junctions between endothelial cells by modulating expression and signaling of VE-cadherin. 31,32 These changes are directly relevant to tumor vascularization since dysfunctional VE-cadherin signaling ultimately promotes invasion angiogenesis and blood vessel permeability.…”

Section: Cell-cell Interactionsmentioning

confidence: 99%

Tissue-Engineered Three-Dimensional Tumor Models to Study Tumor Angiogenesis

Verbridge

Chandler

Fischbach

2010

Tissue Engineering Part A

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Cell-microenvironment interactions play a critical role in the transformation of normal cells into cancer; however, the underlying mechanisms and effects are far from being well understood. Tissue Engineering provides innovative culture tools and strategies to study tumorigenesis under pathologically relevant culture conditions. Specifically, integration of biomaterials, scaffold fabrication, and micro=nano-fabrication techniques offers great promise to reveal the dynamic role of chemical, cell-cell, cell-extracellular matrix, and mechanical interactions in the pathogenesis of cancer. Due to the central importance of blood vessel formation in tumor growth, progression, and drug response, this review will discuss specific design parameters for the development of culture microenvironments to study tumor angiogenesis. Tumor engineering approaches have the potential to revolutionize our understanding of cancer, provide new platforms for testing of anti-cancer drugs, and may ultimately result in improved treatment strategies.

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Parylene peel-off arrays to probe the role of cell–cell interactions in tumour angiogenesis

Cited by 54 publications

References 46 publications

Oxygen-Controlled Three-Dimensional Cultures to Analyze Tumor Angiogenesis

Oxygen-Controlled Three-Dimensional Cultures to Analyze Tumor Angiogenesis

Block-Cell-Printing for live single-cell printing

Tissue-Engineered Three-Dimensional Tumor Models to Study Tumor Angiogenesis

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