2000
DOI: 10.1016/s0005-2736(00)00211-x
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Abstract: A non-ideal lipid binary mixture (dilauroylphosphatidylcholine/distearoylphosphatidylcholine), which exhibits gel/fluid phase coexistence for wide temperature and composition ranges, was studied using photophysical techniques, namely fluorescence anisotropy, lifetime and resonance energy transfer (FRET) measurements. The FRET donor, N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-dilauroylphosphatidylethanol amine, and a short-tailed FRET acceptor, 1,1'-didodecil-3,3,3',3'-tetramethylindocarbocyanine (DiIC12(3)), were s… Show more

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Cited by 65 publications
(62 citation statements)
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“…This conclusion extends that of Loura, et al, 22 that DiI might segregate into the gel/fluid interface. However, the dimension of interfacial domains we have observed is wider than a single molecular interface layer between the phases.…”
Section: S1supporting
confidence: 91%
See 1 more Smart Citation
“…This conclusion extends that of Loura, et al, 22 that DiI might segregate into the gel/fluid interface. However, the dimension of interfacial domains we have observed is wider than a single molecular interface layer between the phases.…”
Section: S1supporting
confidence: 91%
“…AFM has the advantages of nanoscale resolution and the detection of thickness differences amongst membrane phases. Fluorescence microscopy, which involves lipid probes with different affinities to the different phases, allows for color rendering of phase behavior over large regions and, in principle, for application of statistical fluorescence fluctuation technologies to characterize nanodomain dynamics 6,8,9,22,23 .…”
Section: Introductionmentioning
confidence: 99%
“…Local rearrangements of lipid organization in the plasma membrane of PC12 cells (Figure 3) observed using a general lipophilic marker that differently partitions between the ordered and disordered phase of the lipid bilayer (1,1'-dioctadecyl-3,3,3',3'-tetramethylind ocarbocyanine perchlorate dye, DiIC18(5)) [21,38], are consistent with the hypothesis that ELOA may form transient pores in the plasma membrane. …”
Section: Putative Mechanism Of Eloa-induced Cellular Toxicitysupporting
confidence: 76%
“…If these are sufficiently large to be infinite in the FRET scale (i.e., complications resulting form FRET involving molecules in different domains, or boundary effects, are negligible; this is the case if the domains are larger than ~5–10 R 0 ), then the donor decay law is simply a linear combination of the hypothetical decay laws in each phase i DA , phase i (given by Eq. 4), weighed by the relative amount of D in each phase A i (Loura et al, 2000a, 2001):…”
Section: Non-uniform Distribution Of Fluorophores Contains Topologicamentioning
confidence: 99%