Particle Size Influences the Immune Response Produced by Hepatitis B Vaccine Formulated in Inhalable Particles

Thomas, Carin; Gupta, Vivek; Ahsan, Fakhrul

doi:10.1007/s11095-010-0094-x

Cited by 70 publications

(58 citation statements)

References 46 publications

(66 reference statements)

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“…Previous studies on the influence of particle size on the cellular uptake and immune response were carried out with nanoparticulate materials, such as PLGA, [64,65] …”

Section: Synthesis Of the Cnt-based Immunogensmentioning

confidence: 99%

“…[12,13] Triggering an efficient immune response to low-molecular-weight compounds 60 (haptens) is an even more challenging task because they are smaller and lack a peptide structure, so they cannot be presented by MHC II molecules unless they are conjugated to proteins prior to immunization. [14,15] One of the emerging strategies for increasing the efficiency of the phagocytosis step, thereby enhancing the antibody response, is the use of nanomaterials that can increase the size of the antigen 65 and modify its surface properties. Micro/nanoparticles (MPs/NPs) have been shown to be well-suited for increasing the immune response against protein and peptide antigens because the dimensions of particulate systems are comparable to those of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Parra

Abad‐Somovilla

Mercader

et al. 2013

Journal of Controlled Release

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Carbon nanotubes (CNTs) are nanomaterials with interesting emerging applications. Their 25 properties makes CNTs excellent candidates for use as new nanovehicles in drug delivery, immunization and diagnostics. In the current study, we assessed the immune-response-amplifying properties of CNTs to haptens by using azoxystrobin, the first developed strobilurin fungicide, as a model analyte. An azoxystrobin derivative bearing a carboxylated spacer arm (hapten AZc6) was covalently coupled to bovine serum albumin (BSA), and the resulting BSA-AZc6 conjugate was 30 covalently linked to four functionalized CNTs of different shapes and sizes, varying in diameter and length. These four types of CNT-based constructs were obtained using efficient, fast, and easy functionalization procedures based on microwave-assisted chemistry. New Zealand rabbits and BALB/c mice were immunized with BSA-AZc6 alone and with the four CNT-BSA-AZc6 constructs, both with and without Freund's adjuvant. The IgG-type antibody responses were assessed 35 in terms of the titer and affinity, paying special attention to the relationship between the immune response and the size and shape of the employed CNTs. Immunization with CNT-BSA-AZc6 resulted in enhanced titers and excellent affinities for azoxystrobin. More important, remarkable IgG responses were obtained even in the absence of an adjuvant, thus proving the self-adjuvanting capability of CNTs. Immunogens were able to produce strong anti-azoxystrobin immune responses 40 in rabbits even when administered at a BSA-AZc6 conjugate dose as low as 0.05 g. The short and thick CNT-BSA-AZc6 construct produced the best antibody response under all tested conditions.

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“…Previous studies on the influence of particle size on the cellular uptake and immune response were carried out with nanoparticulate materials, such as PLGA, [64,65] …”

Section: Synthesis Of the Cnt-based Immunogensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Parra

Abad‐Somovilla

Mercader

et al. 2013

Journal of Controlled Release

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“…Initial burst release has been considered as a major drawback of PLGA-based particulate vaccine formulations (27). The pores on the particle surface can reduce the antigen encapsulation (19). Particle porosity is also known to influence the antigen release rate (28).…”

Section: Discussionmentioning

confidence: 99%

“…As our objective was to delineate the effect of particle parameters on PAD4 immunogenicity, we mainly concentrated on particles with a mean diameter in nanometer or up to 10 µm. It has been reported that particles greater than 10 µm induce a low humoral antibody response (19). However, the influence of particle size on immunogenicity was not clearly elucidated from nanometer to 10-µm particle populations (20).…”

Section: The Explored Methods For the Preparation Of Plga Particulatementioning

confidence: 99%

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Antibody Response is Differentially Influenced by PLGA-PAD4 Particle Characteristics

Manish

Bhatnagar

2017

Int J Infect

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Background: Anthrax, as a zoonotic disease, is considered a major bioterror threat. Development of effective prophylactic approaches is needed to combat this disease. The protective antigen domain 4 (PAD4) has been extensively studied as a vaccine candidate against anthrax. Previously, we showed that PAD4 can be successfully encapsulated in poly (lactide-co-glycolide) (PLGA)-based particulate systems. Objectives: We aimed to determine if there is an intricate relationship between the parameters of PLGA particulate process and the associated immunogenicity. Methods: In this study, PLGA (50:50) and PLGA (85:15) with a similar molecular weight were used as the polymer variants. Polymer variants were evaluated using 3 adaptations of w/o/w solvent evaporation method. Six variants of PLGA-PAD4 particulate systems were developed and studied for size, antigen content, and immunogenicity. Results: NaCl in the external aqueous phase was required to achieve nonporous and spherical microparticles. Particle size was found to be dependent on the applied methods, and there was no significant difference in size by varying the PLGA compositions. PLGA variants showed a differential behavior with antigen encapsulation in the evaluated methods. The hydrophobic particulate

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Nanopharmacy: Exploratory Methods for Polymeric Materials

Bansal

Sasso

Makwana

et al. 2016

Pharmaceutical Nanotechnology: Innovation and Production

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Particle Size Influences the Immune Response Produced by Hepatitis B Vaccine Formulated in Inhalable Particles

Abstract: HBsAg delivered in PLGA microspheres elicited an increase in immunogenicity, and the magnitude of immune response was more pronounced with smaller particles. Inhalable particles of HBsAg could be a viable approach to needle-free vaccination.

Cited by 70 publications

References 46 publications

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Antibody Response is Differentially Influenced by PLGA-PAD4 Particle Characteristics

Nanopharmacy: Exploratory Methods for Polymeric Materials

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