Particle size control of poly(dl-lactide-co-glycolide) nanospheres for sterile applications

In the development of a drug for intra-articular administration, a sustained-release formulation is desirable since it is difficult to sustain the effects of conventional injections due to fast drug leakage from the joint cavity. In this study, we prepared sustained release gel formulations for intra-articular administration containing indocyanine green (ICG) as a model drug to follow its fate after intra-articular administration in rats with in-vivo imaging system (IVIS). ICG administered as an aqueous solution leaked from the joint cavity in a short time and was excreted out of the body within a day. On the other hand, ICG in the sustained-release formulations was retained and released in the joint cavity for a week. Next, we prepared a sustained-release formulation with hyaluronic acid (HA) as the gel base containing a pain-relief drug (Drug A). We had administered it and other formulations into the rat knee where we injected bradykinin to evaluate their walking distance after 1 and 3 d. The effect of an aqueous solution of Drug A disappeared on day 3. The HA gel formulation without Drug A was more effective than the aqueous solution. The HA gel formulation with Drug A was the most effective; the walking distance was about 85% of the baseline on day 3. This study showed that the gel formulations were effective to sustain the release of a drug in the knee joint, and that the combination of a pain-relief drug with HA gel was effective to improve the mobility of the acute pain model rats.

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“…Now, we are investigating an HA gel formulation containing ICG-microspheres to meet these requirements. [18][19][20][21][22][23][24] …”

Section: Discussionmentioning

confidence: 99%

Development of Intra-knee Joint Sustained-Release Gel Formulation and Evaluation of Its Pharmacological Efficiency in Rats

Noda

Okuda

Ban

et al. 2017

Biological & Pharmaceutical Bulletin

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“…The particle size was higher in the presence of surfactant in the aqueous phase which was due to the formation of surfactant layer on the particle surface. The presence of surfactant may also lead to an increase in the viscosity of aqueous phase which was reported to increase the particle size due to reduction in the rate of counter-diffusion of solvents (Tsukada et al, 2009).…”

Section: Co-current Flow Versus Counter-current Flow Focusingmentioning

confidence: 99%

Preparation of biodegradable polymeric nanoparticles for pharmaceutical applications using glass capillary microfluidics

Othman

Vladisavljević

Nagy

2015

Chemical Engineering Science

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The aim of this study was to develop a new microfluidic approach for the preparation of nanoparticles with tuneable sizes based on micromixing / direct nanoprecipitation in a coaxial assembly of tapered-end glass capillaries. The organic phase was 1 wt% poly(-caprolactone) (PCL) or poly(dl-lactic acid) (PLA) in tetrahydrofuran and the antisolvent was Milli-Q water.The size of nanoparticles was precisely controlled over a range of 190-650 nm by controlling phase flow rates, orifice size and flow configuration (two-phase co-flow or countercurrent flow focusing). Smaller particles were produced in a flow focusing device, because the organic phase stream was significantly narrower than the orifice and remained narrow for a longer distance downstream of the orifice. The mean size of PCL particles produced in a flow focusing device with an orifice size of 200 m, an organic phase flow rate of 1.7 mL h -1 and an aqueous-toorganic flow rate ratio of 10 was below 200 nm. The size of nanoparticles decreased with decreasing the orifice size and increasing the aqueous-to-organic phase flow rate ratio. Due to *Revised Manuscript Click here to view linked References 2 higher affinity for water and amorphous structure, PLA nanoparticles were smaller and exhibited a smoother surface and more rounded shape than PCL particles.

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“…The effect of agitator speed on the properties of an emulsion was studied by Tsukada et al [43]. The authors reported that the particle sizes decreased from 220 nm to 170 nm with increasing agitation speed from 100 rpm to 1000 rpm.…”

Section: Influence Of the Agitation Speed On Nano-emulsion Characterimentioning

confidence: 99%

Membrane emulsification: A promising alternative for vitamin E encapsulation within nano-emulsion

Laouini

Fessi

Charcosset

2012

Journal of Membrane Science

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Particle size control of poly(dl-lactide-co-glycolide) nanospheres for sterile applications

Cited by 44 publications

References 14 publications

Development of Intra-knee Joint Sustained-Release Gel Formulation and Evaluation of Its Pharmacological Efficiency in Rats

Development of Intra-knee Joint Sustained-Release Gel Formulation and Evaluation of Its Pharmacological Efficiency in Rats

Preparation of biodegradable polymeric nanoparticles for pharmaceutical applications using glass capillary microfluidics

Membrane emulsification: A promising alternative for vitamin E encapsulation within nano-emulsion

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