Participation of GA3, ethylene, NO and HCN in germination of Amaranthus retroflexus L. seeds with various dormancy levels

Kępczyński, Jan; Sznigir, Paweł

doi:10.1007/s11738-014-1524-x

Cited by 17 publications

(23 citation statements)

References 34 publications

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“…In addition to hormones, such as gibberellins and ethylene, nitrogen-containing compounds, such as nitrates, nitrites, hydroxylamines, and azides, can also break dormancy in seeds, and it was suggested that the response of seeds to nitrogenous compounds was caused by NO (Hendriks and Taylorson 1974). NO from various donors, such as nitroprusside (SNP), S-nitroso-N-acetylpenicillamine (SNAP), S-nitrosoglutathione (GSNO), and acidified KNO 2 , removes dormancy in apple, Arabidopsis, barley, lettuce, and redroot pigweed seeds (Beligni and Lamattina 2000;Bethke et al 2004;Gniazdowska et al 2010;Liu et al 2011;Kępczyński and Sznigir 2014). NO produced by seeds is now recognized as a signaling molecule involved in regulating germination of dormant and non-dormant seeds in cooperation with plant hormones (Arc et al 2013b;Krasuska et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…A. retroflexus seeds express relative dormancy and therefore germinate completely after harvest at relatively high temperatures, usually 35-40 s C (Kępczyński et al 1996;Liu et al 2011), but do not germinate at lower temperatures until their dormancy is interrupted. There are A. retroflexus seeds which are unable to germinate after harvest or germinated only partially at 35 s C (Schönbeck and Egley 1980;Kępczyński and Sznigir 2014), indicating that the level of dormancy depends on the harvest. A. retroflexus seed dormancy can be removed by dry storage, the effect which depends on the duration and temperature of storage (Schönbeck and Egley 1980;Kępczyński and Sznigir 2014).…”

Section: Introductionmentioning

confidence: 99%

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Interplay between nitric oxide, ethylene, and gibberellic acid regulating the release of Amaranthus retroflexus seed dormancy

2017

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A very small percentage, below 10%, of Amaranthus retroflexus seeds were germinated at 25°C in the light and thus the seeds were considered to be primary dormant. Nitric oxide (NO) applied for only 5 h stimulated germination of these non-deep physiologically dormant seeds. Likewise, ethephon, ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC) and gibberellic acid (GA 3) induced dormancy release. The stimulatory effect of NO, ACC, and GA 3 on germination was associated with increased ethylene production prior to radicle protrusion. The nitric oxide scavenger, cPTIO, antagonized the stimulatory effect of GA 3 , ethephon, and ACC on seed germination, indicating that the presence of endogenous NO is required for dormancy release by these compounds. This scavenger inhibited both germination and ethylene production stimulated by NO and GA 3. An inhibitor of ethylene binding to its receptor, 2,5-norbornadiene (NBD), antagonized the beneficial effect of ethylene and NO, indicating that ethylene action is necessary for the appearance of seed response to these gases. The ACC synthase inhibitor, aminoethoxyvinylglycine (AVG), and the ACC oxidase inhibitor, a-amino-isobutyric acid (AIB), strengthened the effect of NBD on the germination of NOpretreated seeds. Induction of germination of dormant seeds by NO, ethephon or GA 3 was associated with initiation of the cell cycle prior to radicle protrusion. The data presented indicate NO crosstalk with ethylene and GA 3 in regulating dormancy release in A. retroflexus seeds. Keywords Amaranthus retroflexus Á Ethylene Á Germination Á GA 3 Á Nitric oxide Á Primary dormancy Abbreviations ACC 1-Aminocyclopropane-1-carboxylic acid ACO ACC oxidase ABA Abscisic acid AIB a-Amino-isobutyric acid AVG Aminoethoxyvinylglycine cPTIO 2-(4-Carboxyphenyl)-4,4,5,5tetramethylimidazoline-1-oxyl-3-oxide ETH Ethephon GA 3 Gibberellic acid GA s Gibberellins NBD 2,5-Norbornadiene NO Nitric oxide

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interplay between nitric oxide, ethylene, and gibberellic acid regulating the release of Amaranthus retroflexus seed dormancy

2017

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“…It has been previously found that exogenous NO stimulates germination of dormant Arabidopsis (Bethke et al 2004; Libourel et al 2006) and A. retroflexus seeds (Kępczyński and Sznigir 2014; Kępczyński et al 2017), indicating penetration of cells by NO. To confirm that FCM measures the NO level, embryos and leaves were treated with exogenous NO released from an acidified KNO 2 (Yamasaki 2000), at a concentration which was previously used to treat A. retroflexus seeds to increase the level of the compound in cells (Kępczyński et al 2017).…”

Section: Discussionmentioning

confidence: 93%

Application of flow cytometry with a fluorescent dye to measurement of intracellular nitric oxide in plant cells

Kępczyński

Cembrowska-Lech

2018

Planta

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Main conclusionA simple and rapid method involving flow cytometry and NO-specific probe (DAF-FM DA) proved useful for detection and determination of intracellular NO production in Medicago truncatula suspension cells and leaves as well as in cells of Avena fatua, Amaranthus retroflexus embryos and leaves.The measurement of nitric oxide (NO) in plant material is important for examining the regulatory roles of endogenous NO in various physiological processes. The possibility of detecting and determining intracellular NO production by flow cytometry (FCM) with 4-amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM DA), an NO-specific probe in Medicago truncatula cells in suspension and leaves as well as in cells of embryos and leaves of Avena fatua L. or Amaranthus retroflexus L. was explored. To detect and measure NO production by cell suspension or embryos and leaves, the recommended DAF-FM DA concentration is 5 or 10 µM, respectively, applied for 30 min. Exogenous NO increased the intensity of the fluorescent signal in embryos and leaves of both plants, while carboxy-PTIO (cPTIO), an NO scavenger, decreased it. Thus, these results demonstrate that NO can be detected and an increase and a decrease of its intracellular level can be estimated. Wounding was observed to increase the fluorescence signal, indicating an increase in the intracellular NO level. In addition, the levels of exogenous and endogenous ascorbic acid were demonstrated to have no effect on the NO-related fluorescence signal, indicating the signal’s specificity only in relation with NO. The applicability of the proposed method for detection and determination of NO was confirmed (1) by in situ NO imaging in cell suspensions and (2) by determining the NO concentration in embryos and leaves using the Griess reagent. In view of the data obtained, FCM is recommended as a rapid and simple method with which to detect and determine intracellular NO production in plant cells.

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“…Recently the release of redroot pigweed (Amaranthus retroflexus L.) seeds dormancy was also observed in the light in the presence of NO. Light condition of redroot pigweed seed germination enhanced tissues response to phytohormones such as GA or ethylene but not to NO (Kępczyński and Sznigir 2014).…”

Section: Introductionmentioning

confidence: 89%

“Nitrosative Door” in Seed Dormancy Alleviation and Germination

Krasuska

Ciąćka

Andryka-Dudek

et al. 2014

Signaling and Communication in Plants

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Participation of GA3, ethylene, NO and HCN in germination of Amaranthus retroflexus L. seeds with various dormancy levels

Cited by 17 publications

References 34 publications

Interplay between nitric oxide, ethylene, and gibberellic acid regulating the release of Amaranthus retroflexus seed dormancy

Interplay between nitric oxide, ethylene, and gibberellic acid regulating the release of Amaranthus retroflexus seed dormancy

Application of flow cytometry with a fluorescent dye to measurement of intracellular nitric oxide in plant cells

“Nitrosative Door” in Seed Dormancy Alleviation and Germination

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