Partial thermal imidization of polyelectrolyte multilayer cell tethering surfaces (TetherChip) enables efficient cell capture and microtentacle fixation for circulating tumor cell analysis

Ju, Julia A.; Lee, Cornell J; Thompson, Keyata N.; Ory, Eleanor C; Lee, Rachel; Mathias, Trevor J.; Pratt, Stephen J.; Vítolo, Michele; Jewell, Christopher M.; Martin, Stuart S.

doi:10.1039/d0lc00207k

Cited by 11 publications

(20 citation statements)

References 45 publications

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“…Vortex has developed a microfluidic label-free and automated CTC isolation system, which captures cells using vortices and inertial forces ( 20 , 22 ). Cells are collected in suspension in the container of choice and remain intact, which guarantees the integrity of CTC DNA or RNA signatures, for an ideal compatibility with downstream gene mutation or gene expression assays ( 25 , 27 , 34 ). More importantly for this study, the very little number of WBCs collected (<100 WBCs/mL blood) advantageously provides a CTC sample with a very high purity that can be then directly used for EGFR mutation detection ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

Detection of EGFR Mutations in cfDNA and CTCs, and Comparison to Tumor Tissue in Non-Small-Cell-Lung-Cancer (NSCLC) Patients

Liu¹,

Vuppalapaty²,

Wilkerson³

et al. 2020

Front. Oncol.

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Section: Discussionmentioning

confidence: 99%

Detection of EGFR Mutations in cfDNA and CTCs, and Comparison to Tumor Tissue in Non-Small-Cell-Lung-Cancer (NSCLC) Patients

Liu¹,

Vuppalapaty²,

Wilkerson³

et al. 2020

Front. Oncol.

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“…Using microfluidic cell tethering technology (TetherChip) that facilitates McTN imaging with confocal microscopy [ 20 , 21 ] (Fig. 1 a, arrows, Additional file 1 : Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Previous electron microscopy studies have shown that McTNs are only ~ 100 nm wide and highly sensitive to fixation conditions [ 46 ]. In this study, we optimized TetherChip microfluidic cell tethering technology [ 20 , 21 ] to allow a rapid chemical fixation procedure that preserves McTNs on tumor cells, even after isolation from blood samples (Fig. 7 c, d).…”

Section: Discussionmentioning

confidence: 99%

“…For live, tethered cell experiments, TetherChip devices with the addition of lipid moieties (DOTAP) were utilized to tether tumor cells to these surfaces (Ibidi µ-Slide I Luer 0.8, Ibidi, Germany) while preventing tumor cell adhesion. [ 20 , 21 ]. CellMask-Orange stained cells ± Vinorelbine were allowed to tether for 30 min at 37 °C with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

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Microtubule disruption reduces metastasis more effectively than primary tumor growth

Thompson

Ory

et al. 2022

Breast Cancer Res

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Clinical cancer imaging focuses on tumor growth rather than metastatic phenotypes. The microtubule-depolymerizing drug, Vinorelbine, reduced the metastatic phenotypes of microtentacles, reattachment and tumor cell clustering more than tumor cell viability. Treating mice with Vinorelbine for only 24 h had no significant effect on primary tumor survival, but median metastatic tumor survival was extended from 8 to 30 weeks. Microtentacle inhibition by Vinorelbine was also detectable within 1 h, using tumor cells isolated from blood samples. As few as 11 tumor cells were sufficient to yield 90% power to detect this 1 h Vinorelbine drug response, demonstrating feasibility with the small number of tumor cells available from patient biopsies. This study establishes a proof-of-concept that targeted microtubule disruption can selectively inhibit metastasis and reveals that existing FDA-approved therapies could have anti-metastatic actions that are currently overlooked when focusing exclusively on tumor growth.

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“…Decreasing the degree of crosslinking improves PEM gels’ swelling properties, where the crosslinked network would be less compact, and polymer chains become less rigid [ 11 ]. Thus, low degrees of crosslinking allow PEM films to function as hydrogels [ 83 ]. In turn, attaining these properties would aid in applications such as drug encapsulation, where the loading capacity can further increase [ 11 ].…”

Section: Fabrication Of Different Types Of Pe Gelsmentioning

confidence: 99%

Polyelectrolyte Gels: Fundamentals, Fabrication and Applications

Wanasingha¹,

Dorishetty²,

Dutta³

et al. 2021

Gels

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Polyelectrolyte gels are an important class of polymer gels and a versatile platform with charged polymer networks with ionisable groups. They have drawn significant recent attention as a class of smart material and have demonstrated potential for a variety of applications. This review begins with the fundamentals of polyelectrolyte gels, which encompass various classifications (i.e., origin, charge, shape) and crucial aspects (ionic conductivity and stimuli responsiveness). It further centralises recent developments of polyelectrolyte gels, emphasising their synthesis, structure–property relationships and responsive properties. Sequentially, this review demonstrates how polyelectrolyte gels’ flourishing properties create attractiveness to a range of applications including tissue engineering, drug delivery, actuators and bioelectronics. Finally, the review outlines the indisputable appeal, further improvements and emerging trends in polyelectrolyte gels.

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Partial thermal imidization of polyelectrolyte multilayer cell tethering surfaces (TetherChip) enables efficient cell capture and microtentacle fixation for circulating tumor cell analysis

Abstract: TetherChip is an optimized nanosurface comprised of a polyelectrolyte multilayer and a terminal lipid layer in a microfluidics system to enable the study of non-adherent tumor cell behaviors that are relevant to cancer metastasis.

Cited by 11 publications

References 45 publications

Detection of EGFR Mutations in cfDNA and CTCs, and Comparison to Tumor Tissue in Non-Small-Cell-Lung-Cancer (NSCLC) Patients

Detection of EGFR Mutations in cfDNA and CTCs, and Comparison to Tumor Tissue in Non-Small-Cell-Lung-Cancer (NSCLC) Patients

Microtubule disruption reduces metastasis more effectively than primary tumor growth

Polyelectrolyte Gels: Fundamentals, Fabrication and Applications

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