Partial Sequencing of the hrpB and Endoglucanase Genes Confirms and Expands the Known Diversity within the Ralstonia solanacearum Species Complex

Poussier, Stéphane; Prior, Philippe; Luisetti, Jacques; Hayward, Chris; Fegan, Mark

doi:10.1016/s0723-2020(00)80021-1

Cited by 119 publications

(110 citation statements)

References 22 publications

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“…However, ClonalFrame genealogies reconstructed on different gene sets (total data set, housekeeping genes, genes exhibiting low or high r) placed ACH732 either basal to the genealogy or within phylotype IV (data not shown). Incongruence of the phylogenetic assignment of this strain was already reported: the first published egl-based classification placed it within phylotype I (Poussier et al, 2000a), whereas intergenic transcribed tracer-based classification (Fegan et al, 1998) and mutS-based classification considered it a single phylogenetic group . Comparative genomic hybridization results (Remenant et al, 2010) established that ACH732 contained an original gene content, although placed within phylotype IV (Supplementary Figure S6).…”

Section: Discussionmentioning

confidence: 86%

Contrasting recombination patterns and demographic histories of the plant pathogen Ralstonia solanacearum inferred from MLSA

et al. 2011

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We used multilocus sequence analysis (MLSA) on a worldwide collection of the plant pathogenic Ralstonia solanacearum (Betaproteobacteria) to retrace its complex evolutionary history. Using genetic imprints left during R. solanacearum evolution, we were able to delineate distinct evolutionary complex displaying contrasting dynamics. Among the phylotypes already described (I, IIA, IIB, III, IV), eight groups of strains with distinct evolutionary patterns, named clades, were identified. From our recombination analysis, we identified 21 recombination events that occurred within and across these lineages. Although appearing the most divergent and ancestral phylotype, phylotype IV was inferred as a gene donor for the majority of the recombination events that we detected. Whereas this phylotype apparently fuelled the species diversity, ongoing diversification was mainly detected within phylotype I, IIA and III. These three groups presented a recent expanding population structure, a high level of homologous recombination and evidences of long-distance migrations. Factors such as adaptation to a specific host or intense trading of infected crops may have promoted this diversification. Whether R. solanacearum lineages will eventually evolve in distinct species remains an open question. The intensification of cropping and increase of geographical dispersion may favour situations of phylotype sympatry and promote higher exchange of key factors for host adaptation from their common genetic pool.

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Section: Discussionmentioning

confidence: 86%

Contrasting recombination patterns and demographic histories of the plant pathogen Ralstonia solanacearum inferred from MLSA

et al. 2011

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“…Primers were used for amplifying the partial egl and mutS genes as previously described (Poussier et al 2000a;Prior and Fegan 2005). Fifty microliters of the reaction mixture contained 1× Taq MasterMix (Tiangen Biotech), 0.25 μM of each primer, and 50 ng of DNA as the template (Xu et al 2009).…”

Section: Sequencing Of Egl and Muts Genesmentioning

confidence: 99%

“…Sequevar 34 (group E), with reference strain PSS219 (Xu et al 2009), was found in Z1 and Z3. Sequevar 13 (group A), with reference strain JT523 (Poussier et al 2000a), was identified in Z2 and Z3. Sequevar 14 (group B) contained three isolates and was only found in Z3.…”

Section: Distribution Of Sequevars In Different Zonesmentioning

confidence: 99%

The sequevar distribution of Ralstonia solanacearum in tobacco-growing zones of China is structured by elevation

Liu

et al. 2016

Eur J Plant Pathol

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Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease resulting in tremendous losses of economic crops such as plants in the Solanaceae. Recent studies showed that R. solanacearum is spreading from the lowlands to the highlands in China. We studied 97 Chinese R. solanacearum strains that were isolated from four tobacco-growing zones over a wide range of elevations using phylotype specific multiplex polymerase chain reaction (Pmx-PCR) and phylogenetic relationships (egl and mutS). The results showed that all isolates belonged to phylotype I, which were further clustered into eight egl-sequence type groups (egl-group, sequevar): sequevars 13, 14, 15, 17, 34, 44, 54, and 55. In addition, Sequevar 55, found from the highlands, was a new/unknown one. Southeast China (Z3) had the largest number of egl-groups, containing six sequevars. The basin of the Yangzi River (Z1) and southwestern China (Z2) contained five egl-groups. The basin of the Huai River (Z4), near the north of China, where slight bacterial wilt occurred recently, contained a single group, sequevar 15. The distribution of sequevars was associated with elevation. Sequevar 15 was over-represented in lowland elevations, while sequevar 54 and the new/unknown one were only found in areas of moderate to high elevations. This finding suggested that the phylotype I strains infecting tobacco were diverse in China and regional integrated control strategies should be considered.

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“…The conservation of these effectors among evolutionarily distant strains (18) suggests that these T3SS effectors are selected for and are, therefore, probably important for R. solanacearum pathogenicity.…”

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confidence: 99%

Ralstonia solanacearum requires F-box-like domain-containing type III effectors to promote disease on several host plants

Angot

Peeters

Lechner

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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216

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The phytopathogenic bacterium Ralstonia solanacearum encodes a family of seven type III secretion system (T3SS) effectors that contain both a leucine-rich repeat and an F-box domain. This structure is reminiscent of a class of typical eukaryotic proteins called F-box proteins. The latter, together with Skp1 and Cullin1 subunits, constitute the SCF-type E3 ubiquitin ligase complex and control specific protein ubiquitinylation. In the eukaryotic cell, depending on the nature of the polyubiquitin chain, the ubiquitintagged proteins either see their properties modified or are doomed for degradation by the 26S proteasome. This pathway is essential to many developmental processes in plants, ranging from hormone signaling and flower development to stress responses. Here, we show that these previously undescribed T3SS effectors are putative bacterial F-box proteins capable of interacting with a subset of the 19 different Arabidopsis Skp1-like proteins like bona fide Arabidopsis F-box proteins. A R. solanacearum strain in which all of the seven GALA effector genes have been deleted or mutated was no longer pathogenic on Arabidopsis and less virulent on tomato. Furthermore, we found that GALA7 is a host-specificity factor, required for disease on Medicago truncatula plants. Our results indicate that the GALA T3SS effectors are essential to R. solanacearum to control disease. Because the F-box domain is essential to the virulence function of GALA7, we hypothesize that these effectors act by hijacking their host SCF-type E3 ubiquitin ligases to interfere with their host ubiquitin͞proteasome pathway to promote disease.plant pathogen ͉ SCF complex ͉ type III secretion system ͉ virulence

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Partial Sequencing of the hrpB and Endoglucanase Genes Confirms and Expands the Known Diversity within the Ralstonia solanacearum Species Complex

Cited by 119 publications

References 22 publications

Contrasting recombination patterns and demographic histories of the plant pathogen Ralstonia solanacearum inferred from MLSA

Contrasting recombination patterns and demographic histories of the plant pathogen Ralstonia solanacearum inferred from MLSA

The sequevar distribution of Ralstonia solanacearum in tobacco-growing zones of China is structured by elevation

Ralstonia solanacearum requires F-box-like domain-containing type III effectors to promote disease on several host plants

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