Partial replacement of left hemidiaphragm in dogs by either cryopreserved or decellularized heterograft patch

Davari, Hamid Reza; Rahim, Mohammad Bagher; Tanideh, Nader; Sani, Mahsa; Tavakoli, Hamid; Rasekhi, Alireza; Monabati, Ahmad; Koohi-Hosseinabadi, Omid; Gholami, Somayeh

doi:10.1093/icvts/ivw132

Cited by 12 publications

(18 citation statements)

References 23 publications

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“…According to the literature, the hemidiaphragm of a deceased donor was also considered for tissue engineering purposes. Davari et al, [87] evaluated the effectiveness of cryopreservation versus decellularization before implanting derived hemidiaphragm patches in a canine defect model. Briefly, cryopreservation consisted in freezing the grafts at −80 °C and preserving them for up to 2 months; while decellularization was performed according to a detergent–enzymatic protocol consisting of a dH 2 O soaking-phase followed by 4% sodium deoxycholate and 2000 kU of DNase-I in 1 mol/L NaCl.…”

Section: Skeletal Musclementioning

confidence: 99%

Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives

Porzionato

Stocco

Barbon

et al. 2018

IJMS

253

191

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Tissue engineering and regenerative medicine involve many different artificial and biologic materials, frequently integrated in composite scaffolds, which can be repopulated with various cell types. One of the most promising scaffolds is decellularized allogeneic extracellular matrix (ECM) then recellularized by autologous or stem cells, in order to develop fully personalized clinical approaches. Decellularization protocols have to efficiently remove immunogenic cellular materials, maintaining the nonimmunogenic ECM, which is endowed with specific inductive/differentiating actions due to its architecture and bioactive factors. In the present paper, we review the available literature about the development of grafts from decellularized human tissues/organs. Human tissues may be obtained not only from surgery but also from cadavers, suggesting possible development of Human Tissue BioBanks from body donation programs. Many human tissues/organs have been decellularized for tissue engineering purposes, such as cartilage, bone, skeletal muscle, tendons, adipose tissue, heart, vessels, lung, dental pulp, intestine, liver, pancreas, kidney, gonads, uterus, childbirth products, cornea, and peripheral nerves. In vitro recellularizations have been reported with various cell types and procedures (seeding, injection, and perfusion). Conversely, studies about in vivo behaviour are poorly represented. Actually, the future challenge will be the development of human grafts to be implanted fully restored in all their structural/functional aspects.

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Section: Skeletal Musclementioning

confidence: 99%

Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives

Porzionato

Stocco

Barbon

et al. 2018

IJMS

253

191

View full text Add to dashboard Cite

show abstract

“…The procedure of tissue decellularization is to effectively remove cellular components and residual DNA, but keep the physicochemical structure of the ECM to support seeding cells' survival in a 3D architecture [ 25 , 26 ]. Our protocol includes (1) excision of the rat hemidiaphragm in a sterile environment; (2) put the diaphragm in a tube with 40 mL PBS (50 mL, BD); (3) transfer the diaphragm into a 50 mL tube prefilled with 0.5% SDS; (4) attach tube to rocker (Nutator, number 421105, Beckton Dickinson, MD) and rotate for 0.5/sec for 24 hours; (5) transfer the diaphragm to a fresh tube prefilled with 0.5% sodium dodecyl sulfate (SDS) and rotate for 24 hours; (6) transfer the decellularized tissue into a 50 mL tube with sterile PBS and wash during rotation for 24 hours; and (7) leave the tissue in PBS and store at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Decellularized ECM scaffolds have the potential of regenerating the structure and function of their native tissue over commercially available matrices from other tissues. Those decellularized ECM scaffolds have been used in combination with stem cells to construct composite tissues that have been utilized successfully in tissue repair, including diaphragmatic repair [ 23 – 25 ]. While the current practice of PTFE/Gore-tex® patch repair is reasonably effective with acceptable rates of recurrence and infection, a simple biologic tissue could represent an advantage, especially on the large diaphragmatic defects.…”

Section: Introductionmentioning

confidence: 99%

Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model

Liao¹,

Choi

Vojnits

et al. 2017

Stem Cells International

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Tissue engineering is an emerging strategy for repairing damaged tissues or organs. The current study explored using decellularized rat diaphragm scaffolds combined with human amniotic fluid-derived multipotent stromal cells (hAFMSC) to provide a scaffold, stem cell construct that would allow structural barrier function during tissue ingrowth/regeneration. We created an innovative cell infusion system that allowed hAFMSC to embed into scaffolds and then implanted the composite tissues into rats with surgically created left-sided diaphragmatic defects. Control rats received decellularized diaphragm scaffolds alone. We found that the composite tissues that combined hAFMSCs demonstrated improved physiological function as well as the muscular-tendon structure, compared with the native contralateral hemidiaphragm of the same rat. Our results indicate that the decellularized diaphragm scaffolds are a potential support material for diaphragmatic hernia repair and the composite grafts with hAFMSC are able to accelerate the functional recovery of diaphragmatic hernia.

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“…Once deceullularisation has removed cellular material, the remaining ECM retains the native 3D microstructure, molecular composition and growth factors that support skeletal muscle regeneration [25]. Decellularization protocols vary between studies but commonly include the use of detergents and enzymes, such as DNase and Trypsin; the effectiveness of the decellularization protocol is central to minimising scaffold immunogenicity [27][28][29][30][31].…”

Section: Decellularised Scaffoldsmentioning

confidence: 99%

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

Langridge

Butler

2021

J Mater Sci: Mater Med

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Skeletal muscle is capable of regeneration following minor damage, more significant volumetric muscle loss (VML) however results in permanent functional impairment. Current multimodal treatment methodologies yield variable functional recovery, with reconstructive surgical approaches restricted by limited donor tissue and significant donor morbidity. Tissue-engineered skeletal muscle constructs promise the potential to revolutionise the treatment of VML through the regeneration of functional skeletal muscle. Herein, we review the current status of tissue engineering approaches to VML; firstly the design of biocompatible tissue scaffolds, including recent developments with electroconductive materials. Secondly, we review the progenitor cell populations used to seed scaffolds and their relative merits. Thirdly we review in vitro methods of scaffold functional maturation including the use of three-dimensional bioprinting and bioreactors. Finally, we discuss the technical, regulatory and ethical barriers to clinical translation of this technology. Despite significant advances in areas, such as electroactive scaffolds and three-dimensional bioprinting, along with several promising in vivo studies, there remain multiple technical hurdles before translation into clinically impactful therapies can be achieved. Novel strategies for graft vascularisation, and in vitro functional maturation will be of particular importance in order to develop tissue-engineered constructs capable of significant clinical impact.

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Partial replacement of left hemidiaphragm in dogs by either cryopreserved or decellularized heterograft patch

Cited by 12 publications

References 23 publications

Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives

Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives

Tissue Engineering to Repair Diaphragmatic Defect in a Rat Model

Regenerative medicine for skeletal muscle loss: a review of current tissue engineering approaches

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