Partial Purification and Characterization of Proteases from the Visceral Waste of Indian Major Carp, Labeo rohita (Hamilton, 1822)

Abstract

Acidic and alkaline proteases from visceral waste of Labeo rohita (Hamilton, 1822) were isolated, partially purified by ammonium sulphate precipitation followed by dialysis, their kinetics and characteristics studied. The purification fold increased from 1.24 to 2.49 and 1.19 to 1.55 in acidic and alkaline protease respectively along the purification steps. The molecular weight was found in the range of 15-35 kDa and 25-63 kDa respectively in acidic and alkaline proteases. The pH and temperature optima for acidic and alkaline proteases were 3 and 10, at 40°C and 60°C respectively. The Protease activity was decreased by 40% and 60%, when incubated at 90°C for 30 min. Both the proteases showed a decreased activity of more than 50% after incubation with NaCl concentration of 0.5%. Degree of hydrolysis (DH) of the proteases on muscle protein increased with increase of enzyme concentrations. Both soybean trypsin inhibitor and EDTA exhibited high percentage of inhibition, when proteases were incubated with 50 mM of both the inhibitors. The study showed that proteases from Rohu visceral waste of could find use in applications where maximum activity at moderate temperature and low NaCl concentration is desired.

Caracterización y potencial aplicación industrial de extractos enzimáticos de pacú (Piaractus mesopotamicus)

“…Previamente, se ha reportado que los extractos con actividad pepsina de los peces de agua dulce sábalo (Prochilodus lineatus), surubí (Pesudoplatistoma sp. ), pez disco (Symphysodon aequifasciata), carpa india mayor (Labeo rohita) exhibieron actividades 43,4; 205; 0,64 y 2,9 U mg -1 frente al sustrato hemoglobina ácida (Acevedo Gomez et al 2018:2019b, Chong et al 2002, Mohanty et al 2020). En este trabajo, los resultados obtenidos para el EP de pacú evidenciaron un valor intermedio entre los informados por la literatura.…”

“…El rango de pHs con actividad enzimática del EP de pacú (pHs 1,0 a 3,5) resultó similar a lo reportado para los extractos de surubí, lenguado y jaurel amarillo (Candiotto et Silva et al 2022b). La determinación del pH óptimo de una enzima es esencial ya que constituye un indicador para su potencial aplicación en diferentes procesos (Mohanty et al 2020). Estos resultados indican que tanto el EP, como las pepsinas de pacú, podrían ser utilizadas para la hidrólisis de proteínas alimentarias.…”

Autolysis and the endogenous proteinases characterised in beardless barb (Anematichthys apogon) muscle

“…The result suggested that the endogenous proteinase enzymes in beardless barb muscle were able to hydrolyse its muscle proteins at high salt concentration but to a lesser than that with the absence of salt. The relative enzyme activity from visceral waste of Labeo rohita showed a decrease of more than 50% for both acidic and alkaline proteases at NaCl concentration of 0.5% (Mohanty et al ., 2020). Vannabun et al .…”

16S rRNA Sequencing of Lipase Producing Bacterial Strain Isolated from Fish

“…Contents available at Journals Home Page: www.ijzi.net Prachumratana, 2008), catalyses the hydrolysis of long-chain triacylglycerols to form glycerol and fatty acids. Microbial lipases are one of the most essential extracellular enzymes that have drawn the interest of scientists for years due to their broad biotechnological applications (Mohanty et al, 2020). Microbial enzymes have achieved worldwide recognition for their widespread use in diverse sectors of industry.…”