Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (Aranae:Ctenidae)

Estrada-Gómez, Sebastián; Muñoz, Leidy Johana Vargas; Lanchero, Paula; Segura, Cesar

doi:10.3390/toxins7082872

Cited by 23 publications

(22 citation statements)

References 44 publications

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“…Serine proteases are very ubiquitous components in several venoms. Although they are vastly studied in snake venoms [ 115 ], they are also found in many arachnid venoms, as detected by both -omics and experimental approaches [ 42 , 116 , 117 ]. There are several hypotheses for the role of serine proteases in spider venoms.…”

Section: Resultsmentioning

confidence: 99%

An overview of Phoneutria nigriventer spider venom using combined transcriptomic and proteomic approaches

et al. 2018

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Phoneutria nigriventer is one of the largest existing true spiders and one of the few considered medically relevant. Its venom contains several neurotoxic peptides that act on different ion channels and chemical receptors of vertebrates and invertebrates. Some of these venom toxins have been shown as promising models for pharmaceutical or biotechnological use. However, the large diversity and the predominance of low molecular weight toxins in this venom have hampered the identification and deep investigation of the less abundant toxins and the proteins with high molecular weight. Here, we combined conventional and next-generation cDNA sequencing with Multidimensional Protein Identification Technology (MudPIT), to obtain an in-depth panorama of the composition of P. nigriventer spider venom. The results from these three approaches showed that cysteine-rich peptide toxins are the most abundant components in this venom and most of them contain the Inhibitor Cysteine Knot (ICK) structural motif. Ninety-eight sequences corresponding to cysteine-rich peptide toxins were identified by the three methodologies and many of them were considered as putative novel toxins, due to the low similarity to previously described toxins. Furthermore, using next-generation sequencing we identified families of several other classes of toxins, including CAPs (Cysteine Rich Secretory Protein—CRiSP, antigen 5 and Pathogenesis-Related 1—PR-1), serine proteinases, TCTPs (translationally controlled tumor proteins), proteinase inhibitors, metalloproteinases and hyaluronidases, which have been poorly described for this venom. This study provides an overview of the molecular diversity of P. nigriventer venom, revealing several novel components and providing a better basis to understand its toxicity and pharmacological activities.

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Section: Resultsmentioning

confidence: 99%

An overview of Phoneutria nigriventer spider venom using combined transcriptomic and proteomic approaches

et al. 2018

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“…Spider venom extracted from Hippasa partita, Hippasa agelenoides, and Hippasa lycosina demonstrated various enzymatic activities such as proteolysis, hydrolysis of hyaluronic acid, and PLA 2 activity. A similar study associated the cytotoxicity of H. partita venom towards Ehrlich ascites tumor cells with the presence of PLA 2 and sphingomyelinase D [31]. Although only the protein components of the venom was determined in the prior / present study, the possible influence of other non-enzymatic components of the spider venom that are capable of inducing cytotoxicity should not be ruled out.…”

Section: Discussionmentioning

confidence: 89%

Phlogiellus bundokalbo spider venom: cytotoxic fractions against human lung adenocarcinoma (A549) cells

Mayor

Guevarra

Santiago-Bautista

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Spider venom is a potential source of pharmacologically important compounds. Previous studies on spider venoms reported the presence of bioactive molecules that possess cell-modulating activities. Despite these claims, sparse scientific evidence is available on the cytotoxic mechanisms in relation to the components of the spider venom. In this study, we aimed to determine the cytotoxic fractions of the spider venom extracted from Phlogiellus bundokalbo and to ascertain the possible mechanism of toxicity towards human lung adenocarcinoma (A549) cells. Methods: Spider venom was extracted by electrostimulation. Components of the extracted venom were separated by reversed-phase high performance liquid chromatography (RP-HPLC) using a linear gradient of 0.1% trifluoroacetic acid (TFA) in water and 0.1% TFA in 95% acetonitrile (ACN). Cytotoxic activity was evaluated by the MTT assay. Apoptotic or necrotic cell death was assessed by microscopic evaluation in the presence of Hoechst 33342 and Annexin V, Alexa Fluor TM 488 conjugate fluorescent stains, and caspase activation assay. Phospholipase A 2 (PLA 2) activity of the cytotoxic fractions were also measured. Results: We observed and isolated six fractions from the venom of P. bundokalbo collected from Aurora, Zamboanga del Sur. Four of these fractions displayed cytotoxic activities. Fractions AT5-1, AT5-3, and AT5-4 were found to be apoptotic while AT5-6, the least polar among the cytotoxic components, was observed to induce necrosis. PLA 2 activity also showed cytotoxicity in all fractions but presented no relationship between specific activity of PLA 2 and cytotoxicity. Conclusion: The venom of P. bundokalbo spider, an endemic tarantula species in the Philippines, contains components that were able to induce either apoptosis or necrosis in A549 cells.

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“…The spiders were collected from the caves of Bagacay, Surigao in Mindanao, Philippines through hand-grabbing and the use of long forceps. Spiders were maintained in twelve-hour daylight and darkness, fed with Pycnoscelus striatus once per week and provided with water ad libitum (Estrada-Gomez et al, 2015). Spiders were submitted at the Museum of Natural History, University of the Philippines Los Baños for the morphological characterization and identification of the collected spiders.…”

Section: Spider Collection Rearing Maintenance and Identificationmentioning

confidence: 99%

The Venom of Philippine Tarantula (Theraphosidae) Contains Peptides with Pro-Oxidative and Nitrosative-Dependent Cytotoxic Activities against Breast Cancer Cells (MCF-7) In Vitro

Lopez¹,

Aguilar²,

Fernandez³

et al. 2020

Asian Pac J Cancer Prev

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Background: Breast cancer is a multifactorial disease that affects women worldwide. Its progression is likely to be executed by oxidative stress wherein elevated levels of reactive oxygen and nitrogen species drive several breast cancer pathologies. Spider venom contains various pharmacological peptides which exhibit selective activity to abnormal expression of ion channels on cancer cell surface which can confer potent anti-cancer activities against this disease. Methods: Venom was extracted from a Philippine tarantula by electrostimulation and fractionated by reverse phase-high performance liquid chromatography (RP-HPLC). Venom fractions were collected and used for in vitro analyses such as cellular toxicity, morphological assessment, and oxidative stress levels. Results: The fractionation of crude spider venom generated several peaks which were predominantly detected spectrophotometrically and colorimetrically as peptides. Treatment of MCF-7 cell line of selected spider venom peptides induced production of several endogenous radicals such as hydroxyl radicals (•OH), nitric oxide radicals (•NO), superoxide anion radicals (•O 2−) and lipid peroxides via malondialdehyde (MDA) reaction, which is comparable with the scavenging effects afforded by 400 µg/mL vitamin E and L-cysteine (p<0.05). Concomitantly, the free radicals produced decrease the mitochondrial membrane potential and metabolic activity as detected by rhodamine 123 and tetrazolium dye respectively (p>0.05). This is manifested by cytotoxicity in MCF-7 cells as seen by increase in membrane blebbing, cellular detachment, caspase activity and nuclear fragmentation. Conclusion: These data suggest that the Philippine tarantula venom contains peptide constituents exhibiting pro-oxidative and nitrosative-dependent cytotoxic activities against MCF-7 cells and can indicate mechanistic insights to further explore its potential application as prooxidants in cancer therapy.

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Partial Characterization of Venom from the Colombian Spider Phoneutria Boliviensis (Aranae:Ctenidae)

Cited by 23 publications

References 44 publications

An overview of Phoneutria nigriventer spider venom using combined transcriptomic and proteomic approaches

An overview of Phoneutria nigriventer spider venom using combined transcriptomic and proteomic approaches

Phlogiellus bundokalbo spider venom: cytotoxic fractions against human lung adenocarcinoma (A549) cells

The Venom of Philippine Tarantula (Theraphosidae) Contains Peptides with Pro-Oxidative and Nitrosative-Dependent Cytotoxic Activities against Breast Cancer Cells (MCF-7) In Vitro

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