Parenteral immunization of PLA/PLGA nanoparticle encapsulating outer membrane protein (Omp) from Aeromonas hydrophila: Evaluation of immunostimulatory action in Labeo rohita (rohu)

Rauta, Pradipta Ranjan; Nayak, Bismita

doi:10.1016/j.fsi.2015.02.007

Cited by 42 publications

(34 citation statements)

References 30 publications

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“…41 In the present study, lymphocytes were incubated isolated from mouse spleen with concentrations of PHYP nanospheres previously determined to be in the safe range ( Figure 5A). The obtained concentrations were selectively used to assess lymphocyte proliferation, while the T-and B-cells were separately measured synergistically with PHA and LPS ( Figure 5B and C).…”

Section: Discussionmentioning

confidence: 81%

Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

Zheng

Luo

et al. 2016

IJN

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This study aimed to optimize the preparation conditions of pachyman (PHY)-loaded poly( d,l -lactic acid) (PLA) (PHYP) nanospheres by response surface methodology, explore their characteristics, and assess their effects on splenic lymphocytes. Double emulsion solvent evaporation was used to synthesize PHYP nanospheres, and the optimal preparation conditions were identified as a concentration of poloxamer 188 (F68) (w/v) of 0.33%, a concentration of PLA of 30 mg/mL, and a ratio of PLA to drug (w/w) of 10.25:1 required to reach the highest encapsulation efficiency, which was calculated to be 59.10%. PHYP had a spherical shape with a smooth surface and uniform size and an evident effect of sustained release and relative stability. Splenic lymphocytes are crucial and multifunctional cells in the immune system, and their immunological properties could be enhanced significantly by PHYP treatment. This study confirmed that PHY encapsulated in PLA nanospheres had comparatively steady properties and exerted obvious immune enhancement.

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Section: Discussionmentioning

confidence: 81%

Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

Zheng

Luo

et al. 2016

IJN

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“…38 Among these particulate compounds, PLA-based nanospheres have been proved to be easily taken up by APCs (such as DCs) and to modulate immunity to elicit immune responses in vivo. They are able to induce both humoral and cellular responses, in terms of presenting antigens to both MHC class I and class II pathways.…”

Section: Discussionmentioning

confidence: 99%

Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres

Zheng

Qin

et al. 2018

IJN

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Background Poly lactide (PLA) was proved in the last years to be good for use in sustained drug delivery and as carriers for vaccine antigens. In our previous research, pachyman (PHY)-encapsulated PLA (PHYP) nanospheres were synthesized and their function of controlling drug release was demonstrated. Purpose In order to modify the fast drug-release rate of PHY when inoculated alone, the maturation of bone marrow dendritic cells (BMDCs) in vitro and their immunological enhancement in vivo were explored using PHYP nanospheres. Methods The maturation and antigen uptake of BMDCs were evaluated, both alone and with formulated antigen PHYP nanospheres, ie, ovalbumin (OVA)-loaded PHYP nanospheres, as an antigen delivery system, to investigate antigen-specific humoral and cellular immune responses. Results The results indicated that, when stimulated by PHYP, the BMDCs matured as a result of upregulated expression of co-stimulatory molecules; the mechanism was elucidated by tracing fluorescently labeled antigens in confocal laser scanning microscopy images and observing the uptake of nanospheres by transmission electron microscopy. It was further revealed that mice inoculated with OVA-PHYP had augmented antigen-specific IgG antibodies, increased cytokine secretion by splenocytes, increased splenocyte proliferation, and activation of cluster of differentiation (CD)4 + and CD8 + T cells in vivo. Elevated immune responses were produced by OVA-PHYP, possibly owing to the activation and maturation of dendritic cells (in draining lymph nodes). Conclusion It was corroborated that PHY- and/or OVA-encapsulated PLA nanospheres elicited prominent antigen-presenting effects on BMDCs and heightened humoral and cellular immune responses compared with other formulations.

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“…Although different NP vaccines have been used in aquaculture [18,19,52,53,54], few studies have evaluated their ability to release the antigens in vitro [55,56]. It is important that the ability of the NPs to release antigens in vitro be evaluated as this would give insight into their ability to induce a protective immune response in vaccinated fish.…”

Section: Discussionmentioning

confidence: 99%

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

et al. 2016

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The use of oral vaccination in finfish has lagged behind injectable vaccines for a long time as oral vaccines fall short of injection vaccines in conferring protective immunity. Biodegradable polymeric nanoparticles (NPs) have shown potential to serve as antigen delivery systems for oral vaccines. In this study the recombinant outer membrane protein A (rOmpA) of Edwardsiella tarda was encapsulated in chitosan NPs (NP-rOmpA) and used for oral vaccination of Labeo fimbriatus. The rOmpA purity was 85%, nanodiameter <500 nm, encapsulation efficiency 60.6%, zeta potential +19.05 mV, and there was an in vitro release of 49% of encapsulated antigen within 48 h post incubation in phosphate-buffered saline. Empty NPs and a non-formulated, inactivated whole cell E. tarda (IWC-ET) vaccine were used as controls. Post-vaccination antibody levels were significantly (p = 0.0458) higher in the NP-rOmpA vaccinated fish (Mean OD450 = 2.430) than in fish vaccinated with inactivated whole cell E. tarda (IWC-ET) vaccine (Mean OD450 = 1.735), which corresponded with post-challenge survival proportions (PCSP) of 73.3% and 48.28% for the NP-rOmpA and IWC-ET groups, respectively. Serum samples from NP-rOmpA-vaccinated fish had a higher inhibition rate for E. tarda growth on tryptic soy agar (TSA) than the IWC-ET group. There was no significant difference (p = 0.989) in PCSPs between fish vaccinated with empty NPs and the unvaccinated control fish, while serum from both groups showed no detectable antibodies against E. tarda. Overall, these data show that the NP-rOmpA vaccine produced higher antibody levels and had superior protection over the IWC-ET vaccine, showing that encapsulating OmpA in chitosan NPs confer improved protection against E. tarda mortality in L. fimbriatus. There is a need to elucidate the possible adjuvant effects of chitosan NPs and the immunological mechanisms of protective immunity induced by OMPs administered orally to fish.

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Parenteral immunization of PLA/PLGA nanoparticle encapsulating outer membrane protein (Omp) from Aeromonas hydrophila: Evaluation of immunostimulatory action in Labeo rohita (rohu)

Cited by 42 publications

References 30 publications

Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D,L-lactic acid) nanospheres

Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)

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