Parasitological and immunological diagnoses of strongyloidiasis in immunocompromised and non-immunocompromised children at Uberlândia, state of Minas Gerais, Brazil

Paula, Fabiana Martins de; Castro, Elísio de; Gonçalves-Pires, Maria do Rosário de Fátima; Marçal, Maria das Graças; Campos, María; Costa-Cruz, Júlia Maria

doi:10.1590/s0036-46652000000100009

Cited by 39 publications

(29 citation statements)

References 13 publications

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“…Detection of parasite-specific antibodies by indirect immunofluorescence antibody test (IFAT) and enzymelinked immunosorbent assay (ELISA) may be a useful complement to the parasitological diagnosis of strongyloidiasis (Conway et al 1993a, Costa-Cruz et al 1997, De Paula et al 2000, but such tests show cross-reactivity with filaria, schistosomes, hookworm, Ascaris lumbricoides, Trichuris trichiura and Echinococcus (Gam et al 1987, Conway et al 1993a,b, Lindo et al 1994. Western blotting (WB) tests have been applied with more specificity in the immunodiagnosis of strongyloidiasis (Genta et al 1988, Sato et al 1990, Atkins et al 1999, Uparanukraw et al 1999.…”

mentioning

confidence: 99%

Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis

Silva¹,

Barcelos²,

Passos-Lima

et al. 2003

Mem. Inst. Oswaldo Cruz

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confidence: 99%

Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis

Silva¹,

Barcelos²,

Passos-Lima

et al. 2003

Mem. Inst. Oswaldo Cruz

Self Cite

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“…Por ello, se nota un incremento en el desarrollo de estudios que buscan la posibilidad de un estudio serológico, básicamente mediante la técnica de ELISA, que en los últimos años ha alcanzado preferencia de los investigadores debido a su metodología sencilla y que no demanda gran sofisticación de equipos. Por eso decidimos revisar aquellos trabajos que comparaban la técnica de ELISA con los demás métodos parasitológicos ( [13][14][15][16][17][18][19][20][21][22][23][24] ).…”

Section: Discussionunclassified

Utilidad de exámenes parasitológicos y serológicos como métodos de diagnóstico de estrongiloidiosis humana. Ejercicio de metaanálisis

2013

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RESUMEN OBJETIVO: Realizar un metanálisis revisando bibliografía publicada en los últimos 20 años, que comparaba directa o indirectamente los métodos de diagnóstico serológico y parasitológico de infección por Strongyloides stercoralis. MATERIAL Y MÉTODOS: Se trató de construir una tabla tetracórica con la cual calcular los valores de sensibilidad y especificidad de la serología, teniendo a los métodos parasitológicos como prueba de oro. Finalmente se seleccionó aquellas referencias que comparaban la técnica de ELISA con los métodos parasitológicos, buscando información que permitiera calcular los valores predictivos positivo y negativo. RESULTADOS: Aplicando criterios de selección, fueron elegidas 25 referencias. Los valores predictivos positivo y negativo resultaron 85,33% y 97,83%, respectivamente. CONCLUSIÓN: Estos resultados muestran la utilidad potencial del método de ELISA para el diagnóstico de tamizaje de estrongiloidiosis, para facilitar estudios en poblaciones que se encuentren expuestas a esta parasitosis potencialmente letal.Palabras clave: Strongyloides stercoralis; estrongiloidiasis; ELISA; diagnóstico; serología. USEFULNESS OF PARASITOLOGIC AND SEROLOGIC STUDIES IN THE DIAGNO-SIS OF HUMAN STRONGYLOIDIASIS. METAANALYSIS EXERCISE ABSTRACTOBJECTIVE: To conduct metaanalysis by reviewing bibliographic references published during the last 20 years that directly or indirectly compared serologic and parasitologic diagnostic methods for Strongyloides stercoralis infection. MATERIAL AND METHODS: We tried to build a tetracoric table to obtain values of serology sensitivity and specificity, choosing parasitologic methods as gold standard. Finally, those references that compared ELISA with parasitologic methods were selected and information was obtained to calculate positive and negative predictive values. RESULTS: Twenty-five references were chosen by applying selection criteria. Positive and negative predictive values were 85,33% and 97,83%, respectively. CONCLUSION: These results show the potential usefulness of ELISA method as a screening test for strongyloidiasis, in order to facilitate studies in populations exposed to this potentially lethal parasite.

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“…Various factors can influence S. stercoralis prevalence, including the integrity of immunity, socioeconomic status, sanitary and hygiene conditions of individuals, the level of heat and humidity, which affect parasite development in soil [27, 28], and the laboratory methods used for diagnosis [29, 30]. The prevalence of strongyloidiasis in a population can be divided into three categories: sporadic (<1%), endemic (1–5%), and hyperendemic (>5%) [31].…”

Section: Epidemiology Of Strongyloidiasis and Association With Alcmentioning

confidence: 99%

“…ELISAs are the most widely used immunodiagnostic methods and have shown sensitivities and specificities above 70.0%, depending on the screened antigen and antibody isotype [101–104]. However, because of difficulties in the production and the standardization of antigens capable of providing reproducible results, the use of ELISAs remains limited [30]. Uncertainties regarding positive reactions may occur in cases of hidden strongyloidiasis (i.e., low parasite burdens), immunological memory of past infections, or the presence of similar antigens among helminths [104].…”

Section: S Stercoralis Diagnosis and Treatmentmentioning

confidence: 99%

Strongyloides stercoralisInfection in Alcoholic Patients

Teixeira

Pacheco

Souza

et al. 2016

BioMed Research International

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The course of Strongyloides stercoralis infection is usually asymptomatic with a low discharge of rhabditoid larva in feces. However, the deleterious effects of alcohol consumption seem to enhance the susceptibility to infection, as shown by a fivefold higher strongyloidiasis frequency in alcoholics than in nonalcoholics. Moreover, the association between S. stercoralis infection and alcoholism presents a risk for hyperinfection and severe strongyloidiasis. There are several possible mechanisms for the disruption of the host-parasite equilibrium in ethanol-addicted patients with chronic strongyloidiasis. One explanation is that chronic ethanol intake stimulates the hypothalamic-pituitary-adrenal (HPA) axis to produce excessive levels of endogenous cortisol, which in turn can lead to a deficiency in type 2 T helper cells (Th2) protective response, and also to mimic the parasite hormone ecdysone, which promotes the transformation of rhabditiform larvae to filariform larvae, leading to autoinfection. Therefore, when untreated, alcoholic patients are continuously infected by this autoinfection mechanism. Thus, the early diagnosis of strongyloidiasis and treatment can prevent serious forms of hyperinfection in ethanol abusers.

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Parasitological and immunological diagnoses of strongyloidiasis in immunocompromised and non-immunocompromised children at Uberlândia, state of Minas Gerais, Brazil

Abstract: SUMMARYParasitological and immunological diagnoses were part of a study conducted among 151 children, 83 immunocompromised (IC) and 68 non-immunocompromised (non-IC) aged from zero to 12, seen at

Cited by 39 publications

References 13 publications

Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis

Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis

Utilidad de exámenes parasitológicos y serológicos como métodos de diagnóstico de estrongiloidiosis humana. Ejercicio de metaanálisis

Strongyloides stercoralisInfection in Alcoholic Patients

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