Parameters of production and partial characterization of feline interleukin 2

Bauer, Richard; Olsen, Richard G.

doi:10.1016/0165-2427(88)90105-5

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…Methods to evaluate cellular immunity in cats include the lymphocyte transformation test, 21 delayed hypersensitivity reaction (DTH), 19,20 measurement of IL-2 production using IL-2-dependent cell lines, 22 and measurement of interferon (IFN)-g mRNA using the reverse transcriptase polymerase chain reaction (RT-PCR). 17,18 Measurement of the secreted IFN-g is a method that can be easily employed to evaluate the cellular immune response.…”

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confidence: 99%

Development of monoclonal antibodies (MAbs) to feline interferon (fIFN)-γ as tools to evaluate cellular immune responses to feline infectious peritonitis virus (FIPV)

Satoh

Kaku

Satomura

et al. 2011

Journal of Feline Medicine and Surgery

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Feline infectious peritonitis virus (FIPV) can cause a lethal disease in cats, feline infectious peritonitis (FIP). The antibody-dependent enhancement (ADE) of FIPV infection has been recognised in experimentally infected cats, and cellular immunity is considered to play an important role in preventing the onset of FIP. To evaluate the importance of cellular immunity for FIPV infection, monoclonal antibodies (MAbs) against feline interferon (fIFN)-γ were first created to establish fIFN-γ detection systems using the MAbs. Six anti-fIFN-γ MAbs were created. Then, the difference in epitope which those MAbs recognise was demonstrated by competitive enzyme-linked immunosorbent assay (ELISA) and IFN-γ neutralisation tests. Detection systems for fIFN-γ (sandwich ELISA, ELISpot assay, and two-colour flow cytometry) were established using anti-fIFN-γ MAbs that recognise different epitopes. In all tests, fIFN-γ production from peripheral blood mononuclear cells (PBMCs) obtained from cats experimentally infected with an FIPV isolate that did not develop the disease was significantly increased by heat-inactivated FIPV stimulation in comparison with medium alone. Especially, CD8(+)fIFN-γ(+) cells, but not CD4(+)fIFN-γ(+) cells, were increased. In contrast, fIFN-γ production from PBMCs isolated from cats that had developed FIP and specific pathogen-free (SPF) cats was not increased by heat-inactivated FIPV stimulation. These results suggest that cellular immunity plays an important role in preventing the development of FIP. Measurement of fIFN-γ production with the anti-fIFN-γ MAbs created in this study appeared to be useful in evaluating cellular immunity in cats.

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confidence: 99%

Development of monoclonal antibodies (MAbs) to feline interferon (fIFN)-γ as tools to evaluate cellular immune responses to feline infectious peritonitis virus (FIPV)

Satoh

Kaku

Satomura

et al. 2011

Journal of Feline Medicine and Surgery

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“…The presence of interleukin 2 (IL-2)-like activity in feline lymphocyte cultures stimulated with concanavalin A or calcium ionphore A23187 plus phorbol myristate acetate has been reported [11,29,[68][69][70][71]. Feline IL-2 activity was first characterized by Goitsuka et al [72] and was further described by Bauer and Olsen [73]. Feline IL-2 closely resembles rat and human IL-2 in having a molecular weight of approx.…”

Section: (3) Interleukinmentioning

confidence: 99%