Parallel microfluidic networks for studying cellular response to chemical modulation

Liu, Dayu; Wang, Lihui; Zhong, Runtao; Li, Bowei; Ye, Nannan; Liu, Xin; Lin, Bingcheng

doi:10.1016/j.jbiotec.2007.06.014

Cited by 38 publications

(34 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1͑a͔͒. The design of the microfluidic networks was relied mainly on the previous work described by Jeon et al 30 and Liu et al 31 The microscale network on the top plate comprised an upstream gradient generator and a series of downstream inlets for embryonic introduction and waste discharge, which is functionally similar to the central outlet in the multi-pyramidal structure presented by Lin's group. 32 After loading the medium with and without pharmaceutics into the device by syringe pump ͑LSP10-1B, LongerPump, Baoding, China͒, the streams were driven to split, combine, mix, and that repeated several times, which resulted in seven gradient concentrations perpendicular to the flowing direction.…”

Section: A Design and Fabrication Of Microfluidic Devicementioning

confidence: 99%

An integrated microfluidic array system for evaluating toxicity and teratogenicity of drugs on embryonic zebrafish developmental dynamics

et al. 2011

View full text Add to dashboard Cite

Seeking potential toxic and side effects for clinically available drugs is considerably beneficial in pharmaceutical safety evaluation. In this article, the authors developed an integrated microfluidic array system for phenotype-based evaluation of toxic and teratogenic potentials of clinical drugs by using zebrafish ͑Danio rerio͒ embryos as organism models. The microfluidic chip consists of a concentration gradient generator from upstream and an array of open embryonic culture structures by offering continuous stimulation in gradients and providing guiding, cultivation and exposure to the embryos, respectively. The open culture reservoirs are amenable to long-term embryonic culturing. Gradient test substances were delivered in a continuous or a developmental stage-specific manner, to induce embryos to generate dynamic developmental toxicity and teratogenicity. Developmental toxicity of doxorubicin on zebrafish eggs were quantitatively assessed via heart rate, and teratological effects were characterized by pericardial impairment, tail fin, notochord, and SV-BA distance /body length. By scoring the teratogenic severity, we precisely evaluated the time-and dose-dependent damage on the chemical-exposed embryos. The simple and easily operated method presented herein demonstrates that zebrafish embryo-based pharmaceutic assessment could be performed using microfluidic systems and holds a great potential in high-throughput screening for new compounds at single animal resolution.

show abstract

Section: A Design and Fabrication Of Microfluidic Devicementioning

confidence: 99%

An integrated microfluidic array system for evaluating toxicity and teratogenicity of drugs on embryonic zebrafish developmental dynamics

et al. 2011

View full text Add to dashboard Cite

show abstract

“…143,144 By using Kwang's novel gradient method, Lee developed a programmable and fully automatic gold array-embedded gradient optofluidic chip for Raman ELISA analysis. 35 The microfluidic channel matches the size of bacteria very well which is conducive to the cultivation of the bacteria or cell 141,[145][146][147] that offers the possibility of system integration issues for bio/chemical molecular analysis at the trace and even single molecule level. 3,148,149 Walter et al 150 presented a fast, reliable and specific test of bacteria by means of SERS in a microfluidic device (Fig.…”

Section: Biological and Biomedicalmentioning

confidence: 99%

Surface-enhanced Raman scattering microfluidic sensor

Wang

2013

RSC Adv.

View full text Add to dashboard Cite

Surface-enhanced Raman scattering (SERS) is a highly sensitive detection technique used to provide information about chemical and biological trace analytes. The confocal Raman microscope makes it possible to monitor small samples in a narrow microfluidic channel, despite the fact that the scattering intensity of the Raman signal is very weak. Recently, microfluidic chip devices coupled with on-chip SERS detection method and their applications to sensitive chemical and biological analyses have attracted significant attention. In this mini-review, highlights of advances in SERS techniques, microfluidic devices and their applications on the biological/environmental analysis of minute analytes within the recent years are provided to illustrate the impact of this rapidly growing area of research.

show abstract

“…Laminar flow systems are ideal for cell analysis as cells can be exposed to controlled chemical gradients and their biochemical and morphological responses studied in vitro [12]. Laminar flow based linear gradient generation in microbioreactors have been demonstrated in several publications as a way of studying cellular response to chemical stimulation [13][14][15][16][17][18]. Laminar flows are described in Section 3.2 of this review.…”

Section: Introductionmentioning

confidence: 99%