Paracoccidioides brasiliensis exoantigens: recognition by IgG from patients with different clinical forms of paracoccidioidomycosis

Panunto-Castelo, Ademílson; Freitas-da-Silva, G; Bragheto, Imaculada Conceição; Martínez, Roberto; Roque-Barreira, Maria Cristina

doi:10.1016/j.micinf.2003.07.008

Cited by 21 publications

(21 citation statements)

References 20 publications

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“…Isotype-specific IgG1 western blot analysis of the reactivity towards fractionated Paracoccidioides brasiliensis antigens indicated that acute patients consistently recognize a set of antigens that are of approximately 19, 27 and 31kDa, in contrast with recognition of 43 and 75kDa antigens, which was common to both the acute and the chronic form. Similar findings were described by another author 16 , showing that a 38kDa antigen was recognized more frequently and intensely by the serum of acute PCM than by the serum of chronic patients. Another study showed that components of the Paracoccidioides brasiliensis with 27, 34 and 43kDa were reactive towards IgG anti-Paracoccidioides brasiliensis antibodies 13 .…”

Section: Discussionsupporting

confidence: 88%

Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate

Ferreira

Côrrea

Cunha

et al. 2008

Rev. Soc. Bras. Med. Trop.

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In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.

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Section: Discussionsupporting

confidence: 88%

Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate

Ferreira

Côrrea

Cunha

et al. 2008

Rev. Soc. Bras. Med. Trop.

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“…We believe that regardless of differences in isolates or strains, these components are better preserved and produced more homogeneously, as observed with other antigens reported in the literature 8 . The same isolates showed less homogeneity in relation to gp43 determined by capture ELISA (data not shown) and the heterogeneity observed in the percentage of the hMM fraction in the electrophoresis was due to variations in the other components present in CFA (data not shown).…”

Section: Conflict Of Interest Financial Support Referencessupporting

confidence: 74%

“…Panunto-Castelo et al 8 identified hMM antigens with 172 or 160kDa in exoAg from three P. brasiliensis isolates (DGO, C-9 and BAT). In this study, the considered hMM band identified in CFA from the P. brasiliensis isolates presented ~366kDa and is, therefore, a different hMM antigen.…”

Section: Discussionmentioning

confidence: 99%

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High molecular mass fraction in clinical isolates of Paracoccidioides brasiliensis

Fredrich

Nagashima

Pavanelli

et al. 2010

Rev. Soc. Bras. Med. Trop.

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Introduction: Different serum levels of the IgG/IgE for Paracoccidioides brasiliensis high mass molecular (hMM) fraction (~366kDa) in the acute and chronic forms of the disease have been reported. Considering the nonexistence of hMM fraction investigation involving clinical isolates of P. brasiliensis, the present study aimed to investigate the presence of the hMM fraction (~366kDa) in cell free antigens (CFA) from P. brasiliensis clinical isolates. Methods: CFA from 10 clinical isolates and a reference strain (Pb18) were submitted to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by gel image capturing and densitometer analysis. Additionally, CFA from 20 isolates and Pb18 were analyzed by capture ELISA (cELISA) using polyclonal (polAb) or monoclonal (mAb) antibodies to the hMM fraction. Results: The presence of the hMM component was observed in CFA of all samples analyzed by SDS-PAGE/densitometry and by cELISA. In addition, Pearson's correlation test demonstrated stronger coefficients between hMM fraction levels using pAb and mAb (R = 0.853) in cELISA. Conclusions: The soluble hMM fraction was present in all the P. brasiliensis clinical isolates analyzed and the reference strain Pb18, which could be used as a source of this antigen. The work also introduces for first time, the cELISA method for P. brasiliensis hMM fraction detection. Analysis also suggests that detection is viable using polAb or mAb and this methodology may be useful for future investigation of the soluble hMM fraction (~366kDa) in sera from PCM patients.

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“…Falsenegative results for specific antibodies may occur in patients not infected with HIV, especially those with the chronic form of the disease and an unifocal, small lesion (Del Negro et al 2000). Patients with this form of paracoccidioidomycosis have a less intense humoral immunological response than patients with acute/subacute form of the disease (Panunto-Castelo et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients

Bellissimo-Rodrigues¹,

Vitali²,

Martínez

2010

Mem. Inst. Oswaldo Cruz

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Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV. The results were compared to those obtained for 75 non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P. brasiliensis antibodies were detected in 65% (DID), 79% (CIEP) and 95% (ELISA) of the patients with HIV/AIDS, significantly lower rates than those detected in cases of endemic paracoccidioidomycosis, which were 89%, 99% and 100%, respectively. The reactive sera of HIV-infected patients also showed lower anti-P. brasiliensis antibody titres than those of non-HIV-infected patients. Despite the lower intensity of the specific humoral response, serological tests are useful for the diagnosis of opportunistic paracoccidioidomycosis in the HIV/AIDS population. We suggest optimization of the laboratory diagnosis by combining the ELISA test with CIEP or DID

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Paracoccidioides brasiliensis exoantigens: recognition by IgG from patients with different clinical forms of paracoccidioidomycosis

Cited by 21 publications

References 20 publications

Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate

Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate

High molecular mass fraction in clinical isolates of Paracoccidioides brasiliensis

Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients

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