Paper based immunosensing of ovarian cancer tumor protein CA 125 using novel nano-ink: A new platform for efficient diagnosis of cancer and biomedical analysis using microfluidic paper-based analytical devices (μPAD)

Bahavarnia, Farnaz; Saadati, Arezoo; Hassanpour, Soodabeh; Hasanzadeh, Mohammad; Shadjou, Nasrin; Hassanzadeh, Ahmad

doi:10.1016/j.ijbiomac.2019.07.109

Cited by 52 publications

(24 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…But those biosensors need different stages of preparation, accomplished technicians and expensive apparatus [ 15 ]. To solve this problem, paper-based immunoassays have been developed and considered by many researchers [ 16 ]. Paper is one of the best and useful materials because it has great features such as easy manipulation, tools-free, reusable and it is cheap and also it has nice capability to permit liquid substances through its hydrophilic platform without using external power.…”

Section: Introductionmentioning

confidence: 99%

“…So it is used as an important factor in the preparation of paper-based immunosensor [ 17 ]. Paper can use as a large and low-cost matrix for the reagents immobilization and its reaction because the paper has a fibrous porous form can direct aqueous liquid streams by capillary force and supplies a wide surface for immobilization of reagents and a big area for reactions of reagents [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

Farshchi

Saadati

Hasanzadeh

2020

Heliyon

Self Cite

View full text Add to dashboard Cite

Prostate cancer is the most significant reason for deaths in men, outside of lung cancer. The clinical examination of cancer proteins or biomarkers is extremely significant in early examination and monitoring of recurrence of disease after treatment. Biomarkers have expanded great clinical significance owing to their extensive spectra in the identification, elimination, early diagnosis and cure of cancer. In this work, novel, ultrasensitive sandwich-type portable bio device based on citrate-capped silver nanoparticles (Citrate-AgNPs) modified graphene quantum dots (GQDs) nano ink for detection of Prostate specific antigen (PSA) was fabricated. Functionalized cysteamine with gold nanoparticles (Cys-AuNPs) was also utilized to amplify the signal. It provides a good and high external area for the immobilization biotinylated antibody of PSA in the large amount. For the first time, citrate-AgNPs-GQDs nano ink was directly written on the cellulose paper surface (ivory sheet and photographic paper) and modified by Cys-AuNPs. So, final structure of the immunodevices was completed after including of Ab1 and PSA (antigen). The immunosensors were used for the recognition of PSA by using DPVs (differential pulse voltammetry) technique. The obtained low limit of quantification (LLOQ) of the first immunodevice (ivory sheet/Citrate AgNPs-GQDs nano-ink/CysA-Au NPs/Ab1/BSA/PSA/Ab2) was 0.07 μg/L and the linear range for the calibration plot was from 0.07 to 60 μg/L. Also, the achieved LLOQ of the second immunodevice (photographic paper/Citrate AgNPs-GQDs nano-ink/Cys-Au NPs/Ab1/BSA/PSA/Ab2) was 0.05 μg/L with the linear range of 10 to 0.05 μg/L. It is noteworthy that, proposed immunoassay was effectively utilized to the monitoring of PSA glycoprotein in unprocessed human plasma sample. Obtained results show that the constructed immunosensor is able to apply as portable bio device for the clinical analysis of PSA in human plasma samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

Farshchi

Saadati

Hasanzadeh

2020

Heliyon

Self Cite

View full text Add to dashboard Cite

show abstract

“…A relevant giant step on the creation of LCs as sensors useful for cancer prevention comes from an elegant paper of Bahavarnia and co-Workers. Here a paper-based immunosensor has been generated, coupled to a fluidic chamber to propagate the fluid toward the sensor area in which the CA125 protein will be detected by an ELISAbased assay (Bahavarnia et al, 2019). Similar approaches based on the immunosensors have been described in literature, useful to study apoptotic activity (Dang et al, 2019) or soluble immune checkpoint inhibitors (Reza et al, 2019) in biological fluids or clinical samples.…”

Section: First Approaches To Microfluidics Systems: Lcmentioning

confidence: 99%

“…LC biosensors have been recently developed and optimized to be applied in oncoimmunology. A clear example is represented by LC biosensors to detect cancer biomarkers, where the main LC biomaterial is represented by paper and nano-inks (Bahavarnia et al, 2019). Despite the discovery of disparate materials well suited for biological studies, the use of Polydimethylsiloxane (PDMS)-based techniques still remains the elected methodology to fabricate all microfluidic devices, including LC biosensors.…”

Section: First Approaches To Microfluidics Systems: Lcmentioning

confidence: 99%

Oncoimmunology Meets Organs-on-Chip

Mattei

Andreone

Mencattini

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

Oncoimmunology represents a biomedical research discipline coined to study the roles of immune system in cancer progression with the aim of discovering novel strategies to arm it against the malignancy. Infiltration of immune cells within the tumor microenvironment is an early event that results in the establishment of a dynamic cross-talk. Here, immune cells sense antigenic cues to mount a specific anti-tumor response while cancer cells emanate inhibitory signals to dampen it. Animals models have led to giant steps in this research context, and several tools to investigate the effect of immune infiltration in the tumor microenvironment are currently available. However, the use of animals represents a challenge due to ethical issues and long duration of experiments. Organs-on-chip are innovative tools not only to study how cells derived from different organs interact with each other, but also to investigate on the crosstalk between immune cells and different types of cancer cells. In this review, we describe the state-of-the-art of microfluidics and the impact of OOC in the field of oncoimmunology underlining the importance of this system in the advancements on the complexity of tumor microenvironment.

show abstract

“…The results showed to be proportional to the amount of CEA capture with a wide linear range and low detection limit of pg/ mL. Recently, Bahavarnia et al 77 developed a more sensitive paper-based platform for the electrochemical detection CA 125 reaching values as low as 0.78 U/mL in human plasma samples. Wu Y and colleagues also pursued a microfluidic paper-based approach to detect multiple biomarkers (AFP, CEA, CA125 and carbohydrate antigen 153 (CA153)).…”

Section: Portable Point-of-care Devicesmentioning

confidence: 99%

Finding the perfect match between nanoparticles and microfluidics to respond to cancer challenges

Maia

Reis

Oliveira

2020

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

The clinical translation of new cancer theranostic has been delayed by inherent cancer's heterogeneity. Additionally, this delay has been enhanced by the lack of an appropriate in vitro model, capable to produce accurate data. Nanoparticles and microfluidic devices have been used to obtain new and more efficient strategies to tackle cancer challenges. On one hand, nanoparticles-based therapeutics can be modified to target specific cells, and/or molecules, and/or modified with drugs, releasing them over time. On the other hand, microfluidic devices allow the exhibition of physiologically complex systems, incorporation of controlled flow, and control of the chemical environment. Herein, we review the use of nanoparticles and microfluidic devices to address different cancer challenges, such as detection of CTCs and biomarkers, point-of-care devices for early diagnosis and improvement of therapies. The future perspectives of cancer challenges are also addressed herein.

show abstract

Paper based immunosensing of ovarian cancer tumor protein CA 125 using novel nano-ink: A new platform for efficient diagnosis of cancer and biomedical analysis using microfluidic paper-based analytical devices (μPAD)

Cited by 52 publications

References 64 publications

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

Oncoimmunology Meets Organs-on-Chip

Finding the perfect match between nanoparticles and microfluidics to respond to cancer challenges

Contact Info

Product

Resources

About