Paper 17: Regulation of Lysyl Oxidase in the Vaginal Wall: Role of Estrogen, Progesterone, and Pregnancy

Drewes, Peter G.; Marinis, Spyridon I.; Acevedo, Jesús F.; Keller, Patrick W.; Schaffer, J I.; Word, R. Ann

doi:10.1097/01.spv.0000176091.92426.fa

Cited by 4 publications

(6 citation statements)

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“…Thus, to investigate the roles of estrogen and LOX family genes in the vagina, we examined the expression of LOX family genes in accelerated ovarian aging mice undergoing treatment with E2. The expression of LOX and LOXL1 genes was significantly higher in the E2-treated group than in the control group, which is consistent with previous reports (Drewes et al, 2005;Balgobin et al, 2013). The expression of the other LOX family genes (LOXL2, LOXL3, and LOXL4) was also increased in the vaginas of the mice treated with E2.…”

Section: Discussionsupporting

confidence: 81%

“…LOX and LOXL3 protein levels are significantly decreased in POP patients (Alarab et al, 2010;Zhao and Zhou, 2012). LOXL2 mRNA levels are significantly decreased in postmenopausal POP patients compared with asymptomatic postmenopausal controls (Drewes et al, 2005). There exists a close relationship between POP and the expression of LOX family members.…”

Section: Introductionmentioning

confidence: 85%

“…Estrogen has been demonstrated to affect the expression of LOX and LOXL1 genes in the vagina (Drewes et al, 2005;Balgobin et al, 2013), though little is known about its effects on other members of the LOX family. Thus, to investigate the roles of estrogen and LOX family genes in the vagina, we examined the expression of LOX family genes in accelerated ovarian aging mice undergoing treatment with E2.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have revealed that the mRNA expression of LOX and LOXL1 genes in the vaginal wall of mice can be upregulated by estradiol (E2) treatment and LOX protein expression and activity were increased by E2 treatment in rat cardiac fibroblasts (Drewes et al, 2007;Voloshenyuk et al, 2012). E2 can upregulate mRNA expression of LOX and LOXL1 in the vaginal wall of humans (Drewes et al, 2005). E2 treatment also results in increases in LOX mRNA levels in the vagina of guinea pigs after an injury (Voloshenyuk et al, 2012;Balgobin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells

Zong

Jiang

Zhao

et al. 2015

J. Zhejiang Univ. Sci. B

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Abstract:The lysyl oxidase (LOX) family encodes the copper-dependent amine oxidases that play a key role in determining the tensile strength and structural integrity of connective tissues by catalyzing the crosslinking of elastin or collagen. Estrogen may upregulate the expression of LOX and lysyl oxidase-like 1 (LOXL1) in the vagina. The objective of this study was to determine the effect of estrogen on the expression of all LOX family genes in the urogenital tissues of accelerated ovarian aging mice and human Ishikawa cells. Mice and Ishikawa cells treated with estradiol (E2) showed increased expression of LOX family genes and transforming growth factor β1 (TGF-β1). Ishikawa cells treated with TGF-β1 also showed increased expression of LOX family genes. The Ishikawa cells were then treated with either E2 plus the TGF-β receptor (TGFBR) inhibitor SB431542 or E2 alone. The expression of LOX family genes induced by E2 was reduced in the Ishikawa cells treated with TGFBR inhibitor. Our results showed that E2 increased the expression of the LOX family genes, and suggest that this induction may be mediated by the TGF-β signal pathway. E2 may play a role in regulating the expression of LOX family genes.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells

Zong

Jiang

Zhao

et al. 2015

J. Zhejiang Univ. Sci. B

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“…Estrogen can potentially upregulate the expression of LOX and LOXL1 mRNA in the vaginal wall . The mRNA levels of LOX and LOXL1 can increase in the vaginal wall under estrogen treatment . Meanwhile, estrogen is mainly secreted by the ovary.…”

Section: Discussionmentioning

confidence: 99%

Decreased expression of elastin and lysyl oxidase family genes in urogenital tissues of aging mice

Jiang

Zong

Luan

et al. 2014

J of Obstet and Gynaecol

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Lysyl Oxidases are Necessary for Myometrial Contractility and On-Time Parturition in Mice

Ouellette,

Do,

Cohn-Guthrie

et al. 2024

Preprint

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The extracellular matrix (ECM) plays a pivotal role in the maintenance of tissue mechanical homeostasis. Collagens and elastic fibers are the most predominant fibrous ECM proteins providing tissue mechanical function through covalent cross-linking which is mediated by the lysyl oxidase family of enzymes. In this study, the function of lysyl oxidases in maintaining the integrity of the extracellular matrix in the myometrium and its impact on parturition-timing was investigated. Gene and protein expression analyses demonstrate that a sub-set of the lysyl oxidase family of enzymes are highly induced in pregnant myometrium. Inhibition of the activity of the lysyl oxidase family of enzymes through β-aminopropionitrile (BAPN) delays parturition in mice, in part, due to myometrial dysfunction. In BAPN treated mice, the expression of genes encoding contraction associated proteins such as connexin 43, oxytocin receptor and prostaglandin synthase 2 is significantly reduced in the myometrium compared to the untreated control mice. Proteomic analysis revealed that the composition of the ECM is altered in response to BAPN treatment which demonstrates that the inhibition of the activity of lysyl oxidases disrupted the integrity of the myometrial ECM. Our findings demonstrate that the lysyl oxidases-mediated ECM function is necessary for the myometrium to transition from a quiescent to a contractile phenotype at term for on-time parturition.

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Paper 17: Regulation of Lysyl Oxidase in the Vaginal Wall: Role of Estrogen, Progesterone, and Pregnancy

Cited by 4 publications

References 0 publications

Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells

Estradiol plays a role in regulating the expression of lysyl oxidase family genes in mouse urogenital tissues and human Ishikawa cells

Decreased expression of elastin and lysyl oxidase family genes in urogenital tissues of aging mice

Lysyl Oxidases are Necessary for Myometrial Contractility and On-Time Parturition in Mice

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