Palynological study of 45 ditch samples from sedimentary successions penetrated by OSE 1 well located offshore western Niger Delta Basin was undertaken to determine the age of the sediment and reconstruct the paleoclimate and depositional paleoenvironment. All the analyzed samples were composited at 30 ft from stratigraphic depth interval of 6330 to 9010 ft. The lithologic composition comprised mainly of brownish grey or light grey, finegrained shales with a gypsiferous horizon near the bottom. Palynological slides were prepared following standard palynological preparation procedures of sample maceration with hydrochloric (HCl) and hydrofluoric (HF) acids. The samples yielded moderately well-preserved, low to high diversity palynomorph assemblages of 58 taxa comprising 29 pollen grains, 9 pteridophic spores, 17 dinoflagellates, one acritarch, one freshwater algae and a fungi spore. The stratigraphic distribution of microflora assemblages was highly variable. The assemblage abundance was dominated by pollen and spores. The retrieved pollen grains included Monoporites annulatus, Sapotaceoidaepollenites sp. Psilatricolporites crassus, Psilatricolporites sp., Zonocostites ramonae, Retitricolporites irregularis, Monocolpites marginatus, Monocolpites sp., Pachydermites diederixi, Proxapertites cursus, Verrutricolporites rotundiporus, Striatopollis catatumbus, Retistephanocolpites gracilis, Echiperiporites estalae, Echiperiporites sp. and Inaperturopollenites sp. The retrieved spores included Magnastriatites howardi, Acrostichum aureum, Laevigatosporites sp., Polypodiaceoisporites sp., Verrucatosporites sp., Crassoretitriletes vanraadshooveni and Stereisporites sp. The recorded dinoflagellates included Achomosphaera sp., Andalusiella sp., Polysphaeridium zoharyi, Selenopemphix nephroides and Spiniferites sp. Leiosphaeridia sp. was the only acritarch How to cite this paper: Ogbahon, O.A.