Palm vitamin E reduces locomotor dysfunction and morphological changes induced by spinal cord injury and protects against oxidative damage

Zadeh-Ardabili, Parastoo Mojtahed; Rad, Sima Kianpour; Rad, Soheila Kianpour; Khaza’ai, Huzwah; Sanusi, Junedah; Zadeh, Musa-al-Reza Haji

doi:10.1038/s41598-017-14765-3

Cited by 15 publications

(11 citation statements)

References 59 publications

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“…These findings agreed with that of previous studies that evaluated the locomotor and histomorphological parameters of spinal cord trauma in a rodent model [49].…”

Section: Resultssupporting

confidence: 93%

Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

et al. 2021

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Spinal Cord Injury (SCI) promotes a cascade of inflammatory events that are responsible for neuronal death and glial scar formation at the site of the injury, hindering tissue neuroregeneration. Among the main approaches for the treatment of SCI, the use of biomaterials, especially gelatin methacryloyl (GelMA), has been proposed because it is biocompatible, has excellent mechanical properties, favoring cell adhesion and proliferation. In addition, it can act as a carrier of anti-inflammatory drugs, preventing the formation of glial scars. The present work presents the development and in situ application of a light-curing formulation based on GelMA containing a natural extract rich in anti-inflammatory, antioxidant and neuroprotective substances (hydroalcoholic extract of red propolis—HERP) in an experimental model of SCI in rats. The formulations were prepared and characterized by time of UV exposition, FTIR, swelling and degradation. The hydrogels containing 1 mg/mL of HERP were obtained by the exposure to UV radiation of 2 μL of the formulation for 60 s. The locomotor evaluation of the animals was performed by the scale (BBB) and demonstrated that after 3 and 7 days of the injury, the GelMA-HERP group (BBB = 5 and 7) presented greater recovery compared to the GelMA group (BBB = 4 and 5). Regarding the inflammatory process, using histomorphological techniques, there was an inflammation reduction in the groups treated with GelMA and GelMA-HERP, with decreases of cavitation in the injury site. Therefore, it is possible to conclude that the use of GelMA and GelMA-HERP hydrogel formulations is a promising strategy for the treatment of SCI when applied in situ, as soon as possible after the injury, improving the clinical and inflammatory conditions of the treated animals.

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“…These findings agreed with that of previous studies that evaluated the locomotor and histomorphological parameters of spinal cord trauma in a rodent model [49].…”

Section: Resultssupporting

confidence: 93%

Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

et al. 2021

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“…Acute inflammation was produced via injection of 20 µl of 2% carrageenan into the subplantar region of the right hind paw of the mice. [ 32 ] The measurement of thickness was immediately done before subplantar injection, and 1, 3, 4 h thereafter, using a micrometer that was particularly modified for the measurement of thickness. The increase in paw thickness was calculated according to the formula [ 33 ] :

Percentage of inhibition = {[(V_{t} - V_{0})}_{control} - {(V_{t} - V_{0})}_{treated}] / {(V_{t} - V_{0})}_{control}] \times 100,

where ( V t – V 0 ) control is the difference in the size of paw in control mice and ( V t – V 0 ) treated is the difference in the size of paw in mice treated with the treatments.…”

Section: Methodsmentioning

confidence: 99%

Design, synthesis, and molecular docking of novel 3,5‐disubstituted‐1,3,4‐oxadiazole derivatives as iNOS inhibitors

Koksal

Dedeoglu-Erdogan

Bader

et al. 2021

Archiv der Pharmazie

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To obtain new anti‐inflammatory agents, recent studies have aimed to replace the carboxylate functionality of nonsteroidal anti‐inflammatory drugs with less acidic heterocyclic bioisosteres like 1,3,4‐oxadiazole to protect the gastric mucosa from free carboxylate moieties. In view of these observations, we designed and synthesized a series of 3,5‐disubstituted‐1,3,4‐oxadiazole derivatives as inhibitors of prostaglandin E2 (PGE2) and NO production with an improved activity profile. As initial screening, and to examine the anti‐inflammatory activities of the compounds, the inhibitions of the productions of lipopolysaccharide‐induced NO and PGE2 in RAW 264.7 macrophages were evaluated. The biological assays showed that, compared with indomethacin, compounds 5a, 5g, and 5h significantly inhibited NO production with 12.61 ± 1.16, 12.61 ± 1.16, and 18.95 ± 3.57 µM, respectively. Consequently, the three compounds were evaluated for their in vivo anti‐inflammatory activities. Compounds 5a, 5g, and 5h showed a potent anti‐inflammatory activity profile almost equivalent to indomethacin at the same dose in the carrageenan‐induced paw edema test. Moreover, the treatment with 40 mg/kg of 5h produced significant anti‐inflammatory activity data. Furthermore, docking studies were performed to reveal possible interactions with the inducible nitric oxide synthase enzyme. Docking results were able to rationalize the biological activity data of the studied inhibitors. In summary, our data suggest that compound 5h is identified as a promising candidate for further anti‐inflammatory drug development with an extended safety profile.

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“…In fact, in order to improve sensory and autonomic dysfunctions associated with SCI, the potential use of vitamin E was suggested [ 58 ]. Moreover, vitamin E treatment markedly enhanced the hind limb locomotor function, reduced the histopathological alterations and the morphological damage in the spinal cord, and the lowered MDA level and GPX activity in SCI [ 59 ]. On the contrary, combined treatment of vitamins C and E significantly contrasted the effects of spinal cord contusion on oxidative stress, increasing SOD and GPX [ 60 ].…”

Section: Dietary Antioxidants In Spinal Cord Injurymentioning

confidence: 99%

Hormetic Effects of Bioactive Compounds from Foods, Beverages, and Food Dressing: The Potential Role in Spinal Cord Injury

Fedullo

Ciccotti²,

Giannotta³

et al. 2021

Oxidative Medicine and Cellular Longevity

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Spinal cord injury (SCI) is a damage or trauma to the spinal cord resulting in a total or partial loss of motor and sensory function. SCI is characterized by a disequilibrium between the production of reactive oxygen species and the levels of antioxidant defences, causing oxidative stress and neuroinflammation. This review is aimed at highlighting the hormetic effects of some compounds from foods, beverages, and food dressing that are able to reduce oxidative stress in patients with SCI. Although curcumin, ginseng, and green tea have been proposed for SCI management, low levels of antioxidant vitamins have been reported in individuals with SCI. Mediterranean diet includes food rich in vitamins and antioxidants. Moreover, food dressing, including spices, herbs, and extra virgin olive oil (EVOO), contains multiple components with hormetic effects. The latter involves the activation of the nuclear factor erythroid-derived 2, consequently increasing the antioxidant enzymes and decreasing inflammation. Furthermore, EVOO improves the bioavailability of carotenoids and could be a delivery system for bioactive compounds. In conclusion, Mediterranean dressing in addition to plant foods can have an important effect on redox balance in individuals with SCI.

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Palm vitamin E reduces locomotor dysfunction and morphological changes induced by spinal cord injury and protects against oxidative damage

Cited by 15 publications

References 59 publications

Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

Design, synthesis, and molecular docking of novel 3,5‐disubstituted‐1,3,4‐oxadiazole derivatives as iNOS inhibitors

Hormetic Effects of Bioactive Compounds from Foods, Beverages, and Food Dressing: The Potential Role in Spinal Cord Injury

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