Paclobutrazol Improves the Quality of Tomato Seedlings to Be Resistant to Alternaria solani Blight Disease: Biochemical and Histological Perspectives

Shalaby, Tarek; Taha, Naglaa; Taher, Dalia; Metwaly, Metwaly M.; El-Beltagi, Hossam S.; Rezk, Adel A.; El-Ganainy, Sherif Mohamed; Shehata, Wael F.; El-Ramady, Hassan; Bayoumi, Yousry

doi:10.3390/plants11030425

Cited by 21 publications

(11 citation statements)

References 43 publications

(51 reference statements)

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“…This finding may answer the main question in this study: is using nanofertilizers effective in acclimatization and rooting of banana transplants? A strong relationship between plant photosynthetic pigments and nanofertilizers has been reported in the literature [39][40][41][42][43][44][45][46]. Surprisingly, nano-Cu recorded the highest values of photosynthetic pigments and its fluorescence compared to control or nano-Se.…”

Section: Photosynthetic Pigments and Fluorescencementioning

confidence: 89%

Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

et al. 2022

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The production of in vitro banana transplants has become an important practice in the global banana production. Proper and enough nutrients are needed for banana production particularly during the acclimatization period. To avoid the environmental problem resulting from the chemical fertilizers, nanofertilizers of Se and Cu were separately applied during the acclimatization of banana. The biological form of nano-Cu (50 and 100 mg L−1) and nano-Se (25, 50, 75, and 100 mg L−1) were studied on acclimatized banana transplants under greenhouse conditions. Both applied nanofertilizers enhanced the growth of transplant by 10.9 and 12.6% for dry weight after nano-Se and nano-Cu application up to 100 mg L−1, respectively. The survival rate was also increased by increasing applied doses of both nanofertilizers up to 100 mg L−1, whereas the highest survival rate (95.3%) was recorded for nano-Cu. All studied photosynthetic pigments and its fluorescence were improved by applying nanofertilizers. Studied antioxidant enzymatic activities (CAT, PPO, and POX) were also increased. A pH decrease in the growing medium was noticed after applying nano-Cu, which may explain the high bioavailability of studied nutrients (N, P, K, Cu, Fe, Se, and Zn) by banana transplants.

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Section: Photosynthetic Pigments and Fluorescencementioning

confidence: 89%

Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

et al. 2022

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“…Changes of the morphophysiological and biochemical parameters with the use of gibberellins biosynthesis inhibitors is consistent being observed in several agricultural cultures. One of the main purposes is to regulate the excessive growth, increase productivity (TETO et al, 2016) and modulate the plant responses against biotic and abiotic stresses (FAN et al, 2020;MOHAMMADI et al, 2017;SHALABY et al, 2022). Additionally, it has been widely employed in the management of the floral induction in fruit plants (AJMI et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Paclobutrazol as a strategy to induce reproductive precocity in sour passion fruit

et al. 2023

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The adoption of growth regulators can be an alternative method to modulate the quality of the propagation material and obtain reproductive precocity. The objective was to evaluate if a gibberellin biosynthesis inhibitor affects the morphophysiological, biochemical and anatomical parameters of sour passion fruit seedlings and if such alterations affect the reproductive period. Two trials were conducted. In trial I, 40 days after emergence, the seedlings were immersed up to the substrate level in paclobutrazol (PBZ) solutions at concentrations of: 0, 40, 80, 120 and 160 mg L-1. At 45 days after the application of PBZ - DAA, the morphophysiology, biochemistry and anatomy were evaluated. Trail II was conducted in pots, using seedlings from trail I with 45 DAA of PBZ. At 200 days after transplanting, accumulation of shoots and roots fresh and dry mass (g), flowering speed index, total number of reproductive organs and SPAD index were evaluated. In seedlings, restriction of shoot growth induced by PBZ was associated with root increment, seedling quality, photosynthetic pigments and optimization of gas exchange. A shorter period of transition from the juvenile – vegetative phase to reproductive is observed when the seedlings were submitted to PBZ concentrations.

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“…However, how these chemicals work in promoting transformation efficiency remains unclear. Paclobutrazol has been implicated multi-stress ameliorant [ 39 , 40 ], which may weaken plant defense against Agrobacterium . On the other hand, paclobutrazol was reported as a plant growth regulator [ 41 ] and was shown to increase callus formation and embryogenesis [ 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

An Improved Procedure for Agrobacterium-Mediated Transformation of ‘Carrizo’ Citrange

Tang

Liu

et al. 2022

Plants

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Although several protocols for genetic transformation of citrus have been published, it is highly desirable to further improve its efficiency. Here we report treatments of Agrobacterium cells and citrus explants prior to and during co-cultivation process to enhance transformation efficiency using a commercially used rootstock ‘Carrizo’ citrange [Citrus sinensis (L.) Osb. × Poncirius trifoliata (L.) Raf.] as a model plant. We found explants from light-grown seedlings exhibited higher transformation efficiency than those from etiolated seedlings. We pre-cultured Agrobacterium cells in a 1/10 MS, 0.5 g/L 2-(N-morpholino) ethanesulfonic acid (MES) and 100 µM acetosyringone liquid medium for 6 h at 25 °C before used to infect citrus explants. We incubated epicotyl segments in an MS liquid medium containing 13.2 µM 6-BA, 4.5 µM 2,4-D, 0.5 µM NAA for 3 h at 25 °C prior to Agrobacterium infection. In the co-cultivation medium, we added 30 µM paclobutrazol and 10 µM lipoic acid. Each of these treatments significantly increased the efficiencies of transformation up to 30.4% (treating Agrobacterium with acetosyringone), 31.8% (treating explants with cytokinin and auxin), 34.9% (paclobutrazol) and 38.6% (lipoic acid), respectively. When the three treatments were combined, we observed that the transformation efficiency was enhanced from 11.5% to 52.3%. The improvement of genetic transformation efficiency mediated by these three simple treatments may facilitate more efficient applications of transgenic and gene editing technologies for functional characterization of citrus genes and for genetic improvement of citrus cultivars.

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Paclobutrazol Improves the Quality of Tomato Seedlings to Be Resistant to Alternaria solani Blight Disease: Biochemical and Histological Perspectives

Cited by 21 publications

References 43 publications

Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

Paclobutrazol as a strategy to induce reproductive precocity in sour passion fruit

An Improved Procedure for Agrobacterium-Mediated Transformation of ‘Carrizo’ Citrange

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