PABPC4 Broadly Inhibits Coronavirus Replication by Degrading Nucleocapsid Protein through Selective Autophagy

Jiao, Yajuan; Kong, Ning; Wang, Hua; Sun, Dage; Dong, Sujie; Chen, Xiaoyong; Zheng, Hao; Wu, Tong; Yu, Hai; Yu, Lingxue; Huang, Yao‐Wei; Wang, Huan; Sui, Baokun; Zhao, Ling; Liao, Ying; Zhang, Wen; Tong, Guangzhi; Shan, Tongling

doi:10.1128/spectrum.00908-21

Cited by 29 publications

(29 citation statements)

References 67 publications

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“…Autophagy is a self‐degradative process playing a key role in preventing various diseases ( Glick et al, 2010 ; Jiao et al, 2021 ; Lee et al, 2021 ). Beclin1, LC3II, and VPS34 are important genes that participate in autophagosome formation ( Yang and Klionsky, 2009 ), and p62 expression represents autophagosome degeneration after the fusion of autophagosomes with lysosomes, whereas CTSD is a characteristic of lysosomal function ( Salazar et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Diallyl Trisulfide Enhances the Survival of Multiterritory Perforator Skin Flaps

et al. 2022

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The multiterritory perforator flap is one of the widest flap patterns used to repair tissue defects. However, flap necrosis of the distal part is still a challenging issue for plastic surgeons. Diallyl trisulfide (DATS) is an efficient ingredient extracted from garlic, exerting many important effects on different diseases. Our experiment aims to reveal whether DATS has a beneficial effect on the survival of perforator flaps and to explore its mechanism of action. The results showed that DATS enhanced angiogenesis and autophagy and reduced cell apoptosis and oxidative stress, thereby improving the survival rate of skin flaps. After co-administration with autophagy inhibitor 3-methyladenine (3MA), perforator flap survival was further improved. Mechanistically, we showed that PI3K/Akt and AMPK-HIF-1α signaling pathways in flap were activated under DATS treatment. All in all, DATS promoted the survival of multiterritory perforator flaps via the synergistic regulation of PI3K/Akt and AMPK-HIF-1α signaling pathways, and inhibition of DATS-induced autophagy further improves flap survival.

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Section: Discussionmentioning

confidence: 99%

Diallyl Trisulfide Enhances the Survival of Multiterritory Perforator Skin Flaps

et al. 2022

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“…However, metazoans often encode multiple cytoplasmic PABPs; which PABPs play a key role in the replication of the coronavirus genome has not yet been studied clearly. Jiao et al have proved that PABPC4 broadly inhibits coronavirus replication by degrading N protein through selective autophagy [28]. Until now, PABP's effect on the infection of PEDV remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in the absence of a poly(A) tail, PABP binds to the 3 -UTR of Dengue virus to promote translation [27]. However, PABPC4 inhibits PEDV replication by degrading the N protein [28].…”

Section: Introductionmentioning

confidence: 99%

Poly(A)-Binding Protein Cytoplasmic 1 Inhibits Porcine Epidemic Diarrhea Virus Replication by Interacting with Nucleocapsid Protein

Wang

Zheng

et al. 2022

Viruses

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Porcine epidemic diarrhea virus (PEDV) is the etiological agent of porcine epidemic diarrhea (PED) characterized by vomit, watery diarrhea, dehydration and high mortality. Outbreaks of highly pathogenic variant strains of PEDV have resulted in extreme economic losses to the swine industry all over the world. The study of host–virus interaction can help to better understand the viral pathogenicity. Many studies have shown that poly(A)-binding proteins are involved in the replication process of various viruses. Here, we found that the infection of PEDV downregulated the expression of poly(A)-binding protein cytoplasmic 1 (PABPC1) at the later infection stage in Vero cells. The overexpression of PABPC1 inhibited the proliferation of PEDV at transcription and translation level, and siRNA-mediated depletion of PABPC1 promoted the replication of PEDV. Furthermore, mass spectrometry analysis and immunoprecipitation assay confirmed that PABPC1 interacted with the nucleocapsid (N) protein of PEDV. Confocal microscopy revealed the co-localizations of PABPC1 with N protein in the cytoplasm. Taken together, these results demonstrate the antiviral effect of PABPC1 against PEDV replication by interacting with N protein, which increases understanding of the interaction between PEDV and host.

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“…This calls for a better understanding of the interactions between the type I IFN regulatory system and coronaviral components, including the N protein. On the other hand, the N protein has been increasingly explored as a drug target to control viral replication and as a vaccine antigen to induce an effective immune response [ 36 , 48 , 49 , 64 – 67 ]. Zoonotic transmission of coronaviruses is another dimension that needs attention when it comes to developing countermeasures against coronavirus infections.…”

Section: Summary and Future Directionsmentioning

confidence: 99%

Mechanisms of host type I interferon response modulation by the nucleocapsid proteins of alpha- and betacoronaviruses

2022

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Coronaviruses can have a devastating impact on the health of humans and animals. Porcine epidemic diarrhea virus (PEDV) causes extremely high fatality rates in neonatal piglets, whereas severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the current COVID-19 pandemic in humans. As a critical component of the host antiviral innate immune response, type I interferon production and signaling play a very important role, especially in the initial phase of the antiviral responses. Coronaviruses have evolved multiple ways to counteract type I interferon responses. Although the primary functions of the nucleocapsid protein are to facilitate viral RNA replication and package viral genomic RNA into virions, recent studies have shown that the nucleocapsid protein is also involved in virus-host interactions. The aim of this review is to summarize our current understanding of how the nucleocapsid proteins of PEDV and SARS-CoV-2 modulate type I interferon responses. This knowledge will be useful for developing strategies to combat coronavirus infections.

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PABPC4 Broadly Inhibits Coronavirus Replication by Degrading Nucleocapsid Protein through Selective Autophagy

Cited by 29 publications

References 67 publications

Diallyl Trisulfide Enhances the Survival of Multiterritory Perforator Skin Flaps

Diallyl Trisulfide Enhances the Survival of Multiterritory Perforator Skin Flaps

Poly(A)-Binding Protein Cytoplasmic 1 Inhibits Porcine Epidemic Diarrhea Virus Replication by Interacting with Nucleocapsid Protein

Mechanisms of host type I interferon response modulation by the nucleocapsid proteins of alpha- and betacoronaviruses

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