p53 Regulates Apoptosis in Human Retinoblastoma

Nuclear expression of p53 in mature tumor endothelium of retinoblastoma

“…In the present study, human retinoblastoma cells demonstrated a variable immunoreactivity against the anti-p53 antibody (FL-393), showing a similar trend with the results of a previous study using the DO-1 monoclonal antibody (9). When comparing the distribution of p53 immunopositivity with DAPI nuclear counterstaining, the p53 protein in retinoblastoma cells was mainly nuclear, with some cytoplasmic localization.…”

Nuclear expression of p53 in mature tumor endothelium of retinoblastoma

“…In the present study, human retinoblastoma cells demonstrated a variable immunoreactivity against the anti-p53 antibody (FL-393), showing a similar trend with the results of a previous study using the DO-1 monoclonal antibody (9). When comparing the distribution of p53 immunopositivity with DAPI nuclear counterstaining, the p53 protein in retinoblastoma cells was mainly nuclear, with some cytoplasmic localization.…”

Quantitative analysis of apoptosis in retinoblastoma

“…Nork et al. found abundant TUNEL‐positive cells especially at the interphase of the necrotic and viable tumour regions 13 . The same feature was observed in our study.…”

“…Apoptosis was reported to be more prevalent in undifferentiated Rb 7,13 . Similarly, we found a slightly higher rate of apoptosis in our undifferentiated cases than differentiated ones (3.1 and 2.7%, respectively), the difference being statistically not significant.…”

Expression patterns of cyclin D1 and related proteins regulating G1-S phase transition in uveal melanoma and retinoblastoma

“…Retinoblastoma cells were negative for BCL-2, the antidote to PCD; perivascular glial cells and scattered astrocytes within the tumour islands only were positive for BCL-2. This absence of BCL-2, together with the extensive apoptosis observed in the majority of the retinoblastomas, would tend to support the suggestion of Nork et al , that apoptosis in retinoblastoma is most likely p53 induced 49. Our findings, however, contrast with those of Yuge et al , who described weak positivity for BCL-2 in all retinoblastoma cells 57.…”

Nuclear expression of p53 in mature tumor endothelium of retinoblastoma

“…In the present study, human retinoblastoma cells demonstrated a variable immunoreactivity against the anti-p53 antibody (FL-393), showing a similar trend with the results of a previous study using the DO-1 monoclonal antibody (9). When comparing the distribution of p53 immunopositivity with DAPI nuclear counterstaining, the p53 protein in retinoblastoma cells was mainly nuclear, with some cytoplasmic localization.…”

Quantitative analysis of apoptosis in retinoblastoma

“…Nork et al. found abundant TUNEL‐positive cells especially at the interphase of the necrotic and viable tumour regions 13 . The same feature was observed in our study.…”

“…Apoptosis was reported to be more prevalent in undifferentiated Rb 7,13 . Similarly, we found a slightly higher rate of apoptosis in our undifferentiated cases than differentiated ones (3.1 and 2.7%, respectively), the difference being statistically not significant.…”

Expression patterns of cyclin D1 and related proteins regulating G1-S phase transition in uveal melanoma and retinoblastoma

“…Retinoblastoma cells were negative for BCL-2, the antidote to PCD; perivascular glial cells and scattered astrocytes within the tumour islands only were positive for BCL-2. This absence of BCL-2, together with the extensive apoptosis observed in the majority of the retinoblastomas, would tend to support the suggestion of Nork et al , that apoptosis in retinoblastoma is most likely p53 induced 49. Our findings, however, contrast with those of Yuge et al , who described weak positivity for BCL-2 in all retinoblastoma cells 57.…”

Nuclear expression of p53 in mature tumor endothelium of retinoblastoma

“…In the present study, human retinoblastoma cells demonstrated a variable immunoreactivity against the anti-p53 antibody (FL-393), showing a similar trend with the results of a previous study using the DO-1 monoclonal antibody (9). When comparing the distribution of p53 immunopositivity with DAPI nuclear counterstaining, the p53 protein in retinoblastoma cells was mainly nuclear, with some cytoplasmic localization.…”

Quantitative analysis of apoptosis in retinoblastoma

“…Nork et al. found abundant TUNEL‐positive cells especially at the interphase of the necrotic and viable tumour regions 13 . The same feature was observed in our study.…”

“…Apoptosis was reported to be more prevalent in undifferentiated Rb 7,13 . Similarly, we found a slightly higher rate of apoptosis in our undifferentiated cases than differentiated ones (3.1 and 2.7%, respectively), the difference being statistically not significant.…”

Expression patterns of cyclin D1 and related proteins regulating G1-S phase transition in uveal melanoma and retinoblastoma

“…Retinoblastoma cells were negative for BCL-2, the antidote to PCD; perivascular glial cells and scattered astrocytes within the tumour islands only were positive for BCL-2. This absence of BCL-2, together with the extensive apoptosis observed in the majority of the retinoblastomas, would tend to support the suggestion of Nork et al , that apoptosis in retinoblastoma is most likely p53 induced 49. Our findings, however, contrast with those of Yuge et al , who described weak positivity for BCL-2 in all retinoblastoma cells 57.…”