Ozonated Sunflower Oil (OSO) Alleviates Inflammatory Responses in Oxazolone-Induced Atopic Dermatitis (AD)-Like Mice and LPS-Treated RAW 264.7 Cells

Abstract

Ozone, a highly reactive oxidant molecule, is widely used as a complementary therapy for various skin diseases, including wound healing, pressure ulcers, diabetic foot, and infections. However, there is limited research on the effectiveness of ozone for atopic dermatitis (AD). Ozonated sunflower oil (OSO) is an active ingredient obtained from partially ozonated sunflower oil (SO). OSO markedly reduced the LPS-induced increase in IL-1β and nitric oxide (NO) levels in RAW 264.7 mouse macrophage cells. Oxazolone (OXZ) was applied to hairless mice to induce AD-like skin symptoms and immune response. OSO significantly alleviated the OXZ-induced increases in the number of infiltrating mast cells, epidermal thickness, AD symptoms, thymic stromal lymphopoietin (TSLP), and filaggrin, as well as the serum levels of NO, IgE, IL-1β, and TNF-α. Furthermore, OSO inhibited the IL-4/STAT3/MAPK pathway and the expression of NF-κB. Our results suggest that OSO treatment could relieve AD-mediated skin damage through its anti-inflammatory and antioxidant activities. Therefore, it can be used as a therapeutic agent against AD-related skin diseases.

Multiplicative Effects of Essential Oils and Other Active Components on Skin Tissue and Skin Cancers

“…Ozonated HA-PO possesses an anti-inflammatory effect on the skin diseases of mice and humans [78]. The symptoms of AD, such as scaling, excoriation, erythema, edema, epidermal thickness, infiltration of mast cell, serum levels of IgE, and spleen weight and lymph node length are decreased by treatment with ozonated HA-PO in an oxazolone (Oxz)-induced AD mouse model [79]. In addition, skin hydration function and FLG are recovered by treatment with ozonated HA-PO through the downregulation of IL-4/signal transducers and activators of the transcription (STAT) 3/extracellular signalregulated kinase (ERK) pathway in an Oxz-induced AD mouse model [79].…”

“…The symptoms of AD, such as scaling, excoriation, erythema, edema, epidermal thickness, infiltration of mast cell, serum levels of IgE, and spleen weight and lymph node length are decreased by treatment with ozonated HA-PO in an oxazolone (Oxz)-induced AD mouse model [79]. In addition, skin hydration function and FLG are recovered by treatment with ozonated HA-PO through the downregulation of IL-4/signal transducers and activators of the transcription (STAT) 3/extracellular signalregulated kinase (ERK) pathway in an Oxz-induced AD mouse model [79]. Moreover, the levels of nitrite oxide (NO) and iNOS are inhibited by treatment with ozonated HA-PO through downregulation of IL-1β and TNF-α via MAPK and the NF-κB pathway in serum and skin tissues in an Oxz-induced AD mouse model [79].…”

“…In addition, skin hydration function and FLG are recovered by treatment with ozonated HA-PO through the downregulation of IL-4/signal transducers and activators of the transcription (STAT) 3/extracellular signalregulated kinase (ERK) pathway in an Oxz-induced AD mouse model [79]. Moreover, the levels of nitrite oxide (NO) and iNOS are inhibited by treatment with ozonated HA-PO through downregulation of IL-1β and TNF-α via MAPK and the NF-κB pathway in serum and skin tissues in an Oxz-induced AD mouse model [79].…”

Oral Supplementation of Ozonated Sunflower Oil Augments Plasma Antioxidant and Anti-Inflammatory Abilities with Enhancement of High-Density Lipoproteins Functionality in Rats

“…In addition, a remarkable effect of OSO in treating endometriosis in mares [12,16] and demodicosis in dogs [13] logged the broad-spectrum antimicrobial role of OSO. Besides the antimicrobial effect, we have recently disclosed the curative effect of OSO against atopic dermatitis (AD) in the mouse model [17], where we observed that the topical application of OSO significantly affected the infiltration of mast cells, filaggrin, and thymic stromal lymphopoietin (TSLP). In addition, OSO was logged for diminished serum Ig E, TNF-α, and IL-1β levels and displayed a significant antioxidant and anti-inflammatory effect by inhibiting the IL-4/STAT3/MAPK pathway and expression of NFκB [17].…”