Oxymatrine ameliorates rheumatoid arthritis by regulation of Tfr/Tfh cell balance viatheTLR9‐MyD88‐STAT3 signaling pathway

Abstract

Background Oxymatrine (OMT) is one of the authentic Chinese herbal medicines which has rich and complex active ingredients. however,the relevant potential targets of oxymatrine on rheumatoid arthritis and the mechanism remains unreported. The aim of this study was to determine the regulation of oxymatrine on rheumatoid arthritis using a collagen‐induced arthritis (CIA) mice models and blood samples from RA patients. Results Our results indicated that Tfr cells in RA patients express low levels of Blimp‐1 and CTLA‐4. Oxymatrine treatment of CIA mice alleviated joint swelling, reduced the arthritis score, and improved joint damage. While flow cytometry results showed that oxymatrine treatment reduced Tfh cells and B cells, and increased Tfr cells in the spleen of CIA mice. In addition, oxymatrine treatment significantly down‐regulated the expression of TLR9（Toll‐like Receptors 9), IL‐21, MyD88, STAT3, p‐STAT3, and CXCR5 in the synovial tissues of CIA mice, and up‐regulated the expression of Foxp3, Blimp‐1, and CTLA‐4. Conclusion Oxymatrine can alleviate rheumatoid arthritis by regulating the TLR9‐MyD88‐STAT3 signaling pathway to maintain immune balance between Tfr‐Tfh cells. © 2023 Society of Chemical Industry.

Oxymatrine Modulation of TLR3 Signaling Pathway: A Dual-Action Mechanism against H9N2 Avian Influenza Virus and Immune Regulation

“…Oxymatrine (OMT), an active monomer extracted from various TCMs including Sophora avescens Ait and Radix Sophorae Tonkinensis (Bi et al, 2012, Liang et al, 2023, has demonstrated e cacy in managing chronic hepatitis B (Lu et al, 2004). Besides its hepatoprotective role, OMT exhibits potential against tumorigenesis, in ammation, and bacterial infections (Li et al, 2022, Cao et al, 2023, Zhang et al, 2023a. Notably, in Hepatitis B virus (HBV) transgenic mice liver (Chen et al, 2001), OMT reduces levels of HBsAg and HBcAg, and as a potent immunosuppressant, it curtails replication and in ammation of type A in uenza virus via the TLR4, p38 MAPK, and NF-κB pathways (Dai et al, 2018).…”

Oxymatrine Modulation of TLR3 Signaling Pathway: A Dual-Action Mechanism Against H9N2 Avian Influenza Virus and Immune Regulation

“…Oxymatrine (OMT), an active monomer extracted from various TCMs including Sophora avescens Ait and Radix Sophorae Tonkinensis (Bi et al, 2012, Liang et al, 2023, has demonstrated e cacy in managing chronic hepatitis B (Lu et al, 2004). Besides its hepatoprotective role, OMT exhibits potential against tumorigenesis, in ammation, and bacterial infections (Li et al, 2022, Cao et al, 2023, Zhang et al, 2023a. Notably, in Hepatitis B virus (HBV) transgenic mice liver (Chen et al, 2001), OMT reduces levels of HBsAg and HBcAg, and as a potent immunosuppressant, it curtails replication and in ammation of type A in uenza virus via the TLR4, p38 MAPK, and NF-κB pathways (Dai et al, 2018).…”

Oxymatrine Modulation of TLR3 Signaling: A Dual-Action Mechanism for H9N2 Avian Influenza Virus Defense and Immune Regulation

“…Traditional Chinese medicine (TCM) offers a distinct perspective on antiviral therapy, emphasizing treatments that are less likely to induce drug resistance. Oxymatrine (OMT), a quinolizidine alkaloid extracted from the roots of Sophora flavescens Aiton and Sophora tonkinensis Gagnep (Fabaceae) [16,17], exhibits a broad spectrum of biological activities [18], including hepatoprotection, anti-tumorigenesis, anti-inflammation, and antibacterial effects [19][20][21]. Its capacity to modulate the immune response and inhibit viral replication of hepatitis B virus (HBV) in transgenic mice underscores its potential as an immunomodulatory agent [22].…”