Oxidative Stress Responses and Recovery Patterns in the Liver of Oreochromis niloticus Exposed to Chlorpyrifos-Ethyl

Oruç, Elif Özcan

doi:10.1007/s00128-012-0548-4

Cited by 39 publications

(16 citation statements)

References 46 publications

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“…Significant interaction effects between chlorpyrifos and vitamin E were observed for 12 dipeptides. Collectively, these findings are in agreement with published studies demonstrating chlorpyrifos induces oxidative stress in multiple model systems [36,37,38,39,40]. Alpha tocopherol supplementation at the studied concentration did not counteract these effects, suggesting that vitamin E, except its ability to partially restore dipeptide levels, has a relatively limited ability to rescue chlorpyrifos-induced oxidative stress in salmon hepatocytes.…”

Section: Discussionsupporting

confidence: 91%

“…No effect of vitamin E supplementation was however seen on PDK2 transcription in the cells. CuZn superoxide dismutase (SOD1) and catalase (CAT), suggested markers for longer-term chlorpyrifos exposure (15 days) in the Nile tilapia ( Oreochromis niloticus ) by Oruc [36], were not among the affected transcripts in cultured Atlantic salmon hepatocytes. To see effects on typical oxidative stress response genes, fish studies suggest that longer-term chlorpyrifos exposure is needed [37].…”

Section: Discussionmentioning

confidence: 99%

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Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon

Berntssen¹,

Søfteland²

2015

PLoS ONE

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The aim of this study was to elucidate how vitamin E (alpha tocopherol) may ameliorate the toxicity of the pesticide chlorpyrifos in Atlantic salmon. Freshly isolated hepatocytes were exposed to vitamin E, chlorpyrifos or a combination of vitamin E and chlorpyrifos (all 100 μM). Transcriptomics (RNA-seq) and metabolomics were used to screen for effects of vitamin E and chlorpyrifos. By introducing vitamin E, the number of upregulated transcripts induced by chlorpyrifos exposure was reduced from 941 to 626, while the number of downregulated transcripts was reduced from 901 to 742 compared to the control. Adding only vitamin E had no effect on the transcriptome. Jak-STAT signaling was the most significantly affected pathway by chlorpyrifos treatment according to the transcriptomics data. The metabolomics data showed that accumulation of multiple long chain fatty acids and dipeptides and amino acids in chlorpyrifos treated cells was partially alleviated by vitamin E treatment. Significant interaction effects between chlorpyrifos and vitamin E were seen for 15 metabolites, including 12 dipeptides. The antioxidant had relatively modest effects on chlorpyrifos-induced oxidative stress. By combining the two data sets, the study suggests that vitamin E supplementation prevents uptake and accumulation of fatty acids, and counteracts inhibited carbohydrate metabolism. Overall, this study shows that vitamin E only to a moderate degree modifies chlorpyrifos toxicity in Atlantic salmon liver cells.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon

Berntssen¹,

Søfteland²

2015

PLoS ONE

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“…The fish were randomly stocked in five groups (2 aquaria/group, duplicates) where the SP free diet was fed to the control group while the second group was fed the basal diet supplemented with 1% SP (HerbaForce, Lewes, UK) without CPF (48%, El Nasr chemical Co., Abu-Rawash, Egypt) exposure. Additionally, CPF (15 µg/L) was added as indicated by Oruc [23] to water and fish were fed the basal diet supplemented with SP at 0, 0.5, and 1% (third, fourth, and fifth groups, respectively). The glass aquaria were siphoned daily to remove the feces and remaining diets and refilled with CPF mixed water.…”

Section: Fish Diet Chlorpyrifos (Cpf) and Experimental Designmentioning

confidence: 99%

Spirulina platensis Reduced Oxidative Damage Induced by Chlorpyrifos Toxicity in Nile Tilapia (Oreochromis niloticus)

Abdel-Daim

Dawood

Elbadawy

et al. 2020

Animals

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Due to the numerous pharmacological impacts of Spirulina platensis (SP), the effects of SP on the oxidative status of Nile tilapia farmed under chlorpyrifos (CPF) ambient toxicity were considered in this study. Fish (60 ± 6.1 g) was randomly stocked in five groups where the SP free diet was fed to the control group while the second one was fed 1% SP without CPF exposure. Additionally, CPF (15 μg/L) was added in water and fish were fed with SP at 0, 0.5, and 1% (third, fourth, and fifth groups, respectively). Samples of blood and gills, kidneys, and liver tissues were assayed for biochemical measurements. Fish exposed to CPF exhibited significant (p ≤ 0.05) increments of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), cholesterol, urea, creatinine, and malondialdehyde (MDA), while significantly decreased total protein, albumin, and antioxidative enzyme activities (superoxide dismutase (SOD) and catalase (CAT) were observed in tilapia exposed to CPF (p ≤ 0.05). In contrast, SP feeding resulted in decreased levels of ALT, AST, ALP, cholesterol, urea, and creatinine as well as increased total protein, albumin, SOD, and CAT activities. Based on the obtained results, it can be suggested that SP is efficient in protecting Nile tilapia from CPF toxicity by increasing the antioxidative response.

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“…Bantu et al () reported an increase in SOD activity in liver of Labeo rohita after 15 days of exposure to chlorantraniliprole. Similarly, chlorpyrifos exposure also caused significant increases in SOD activity in liver tissues of Oreochromis niloticus (Oruc, ).…”

Section: Discussionmentioning

confidence: 99%

Sublethal toxicity of quinalphos on oxidative stress and antioxidant responses in a freshwater fish Cyprinus carpio

Hemalatha

Amala

Rangasamy

et al. 2015

Environmental Toxicology

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Extensive use of quinalphos, an organophosphorus pesticide, is likely to reach the aquatic environment and thereby posing a health concern for aquatic organisms. Oxidative stress and antioxidant responses may be good indicators of pesticide contamination in aquatic organisms. The data on quinalphos induced oxidative stress and antioxidant responses in carps are scanty. This study is aimed to assess the two sublethal concentrations of quinalphos (1.09 and 2.18 μL L ) on oxidative stress and antioxidant responses of Cyprinus carpio for a period of 20 days. In liver, the malondialdehyde level was found to be significantly increased in both the concentrations. The results of the antioxidant parameters obtained show a significant increase in superoxide dismutase, catalase, and glutathione-S-transferase activity in liver of fish. These results demonstrate that environmentally relevant levels of the insecticide quinalphos can cause oxidative damage and increase the antioxidant scavenging capacity in C. carpio. This may reflect the potential role of these parameters as useful biomarkers for the assessment of pesticide contamination. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1399-1406, 2016.

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Oxidative Stress Responses and Recovery Patterns in the Liver of Oreochromis niloticus Exposed to Chlorpyrifos-Ethyl

Cited by 39 publications

References 46 publications

Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon

Modifying Effects of Vitamin E on Chlorpyrifos Toxicity in Atlantic Salmon

Spirulina platensis Reduced Oxidative Damage Induced by Chlorpyrifos Toxicity in Nile Tilapia (Oreochromis niloticus)

Sublethal toxicity of quinalphos on oxidative stress and antioxidant responses in a freshwater fish Cyprinus carpio

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