Oxidative stress modulates the organization of erythrocyte membrane cytoskeleton

Olszewska, Maria; Wiatrow, Jerzy; Bober, Joanna; Stachowska, E.; Gołembiewska, Edyta; Jakubowska, Katarzyna; Stańczyk-Dunaj, Małgorzata; Pietrzak-Nowacka, Maria

doi:10.5604/17322693.1005677

Cited by 18 publications

(14 citation statements)

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“…The higher content of unsaturated fatty acids leads to their greater propensity to be oxidized. Oxidative stress leads also to alteration in membrane cytoskeleton [ 15 ]. In our previous work, we showed changes in dynamics and conformation state of proteins in plasma membrane of erythrocytes from CRF patients.…”

Section: Introductionmentioning

confidence: 99%

Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure

Pieniążek

Gwoździński

2015

Oxidative Medicine and Cellular Longevity

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The aim of this study was to evaluate the properties of internal components of erythrocytes in chronic renal failure (CRF) patients undergoing hemodialysis (HD) in comparison to control subjects. For investigation of conformational state of hemoglobin and nonheme proteins (NHP) the maleimide spin label (MSL) in electron paramagnetic resonance (EPR) was applied. The studies were performed using MSL in whole cells and hemolysate as well as proteins separated by ion exchange chromatography and checked by electrophoresis. Additionally the level of –SH groups in hemolysate and isolated internal proteins of CRF erythrocytes was determined using 4,4′-dithiodipyridine. All measurements were performed before and after hemodialysis. Oxidative stress accompanying CRF/hemodialysed patients caused a significant decrease in the mobility of internal components inside erythrocytes indicated by MSL (P < 0.02). The significant decrease in mobility of spin labeled HbA1c and HbA both before and after HD (P < 0.0002) as well as in nonheme proteins before hemodialysis (P < 0.05) versus control was indicated. Decrease in mobility of internal components of erythrocytes was accompanied by loss of thiols before and after hemodialysis versus control in NHP (P < 0.05), HbA1c (P < 0.0002), and HbA (P < 0.0005). These findings showed oxidative influence of hemodialysis on hemoglobins and internal nonheme proteins in erythrocytes of CRF patients.

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Section: Introductionmentioning

confidence: 99%

Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure

Pieniążek

Gwoździński

2015

Oxidative Medicine and Cellular Longevity

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“…However, the production of ROS as well as the percentage of apoptotic cells suggests deleterious effects on cellular health that ultimately may reduce their overall viability . The contrasted results from Calcein AM ® , CellROX ® , and Annexin V ® could suggest that increased ROS production activates signaling cascades that are dependent on growth factors promoting cell cycle progression that will lead to increased cell metabolism and high calcein activity . While cellular injury continues via ROS induction, the apoptotic pathways induce phosphatidyl serine to be expressed in the outer leaflet of the intact cell membrane, whereby calcein cleavage is kept internalized.…”

Section: Discussionmentioning

confidence: 99%

“…17 The contrasted results from Calcein AM V R , CellROX V R , and Annexin V V R could suggest that increased ROS production activates signaling cascades that are dependent on growth factors promoting cell cycle progression that will lead to increased cell metabolism and high calcein activity. 44,45 While cellular injury continues via ROS induction, the apoptotic pathways induce phosphatidyl serine to be expressed in the outer leaflet of the intact cell membrane, whereby calcein cleavage is kept internalized. It should be pointed out that the 25% subpopulation of apoptotic cells exposed to MBC is suggestive that the cells are able to keep their healthy metabolism, while at the same time, the MBC was still able to induce damage.…”

Section: Discussionmentioning

confidence: 99%

Synthesis andin vitrosafety assessment of magnetic bacterial cellulose with porcine aortic smooth muscle cells

Pastrana

Cooper

Alucozai

et al. 2016

J Biomedical Materials Res

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Bacterial cellulose (BC) has been used as a scaffold for tissue regeneration (TR). Improving functional TR requires highly selective strategies for specific cell attraction. Embedding iron oxide nanoparticles into a BC matrix can drive magnetically labeled cells to specific tissues where they may begin to heal injured tissue. This article focuses on characterization and in vitro toxicity assessment of magnetic BC (MBC). We proposed to detect the production of radical oxygen species (ROS), esterase activity, and apoptosis to study cytotoxic interactions of MBC within its bioenvironment. Morphological characterization was performed using scanning electron microscopy where evidence shows that the diameter of MBC fibers compared to BC fibers was 33% smaller, and the pore areas were 25% bigger. Cytotoxicity assays in porcine aortic smooth muscle cells exposed for 24 hours to BC, MBC, and poly(ethylene glycol)-coated MBC (MBC-PEG) reveals 96% viability and 9% ROS production for MBC-PEG. In contrast, 25% of cells exposed to MBC were apoptotic, suggesting that even when the cells were metabolically active, MBC can induce damage. These outcomes support the need for more integral assessment in the hopes of assessing the potential biosafety and uses of nanocomposites for TR. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2801-2809, 2016.

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“…These infringements were caused by an indirect effect of acetaldehyde and ethanol (via generation of free radical species) and a direct effect of acetaldehyde on erythrocyte protein modification. Under oxidative stress, cytoskeletal proteins can be aggregated with hemoglobin (Olszewska et al, 2012), inducing an alteration of gas exchange in RBCs.…”

Section: Discussionmentioning

confidence: 99%

The Oxidized proteins and activity of the Cl–/HCО3– exchanger in erythrocytes of patients with acute alcohol intoxication

et al. 2019

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Background: The purpose of this research was to study the morphological properties and the products of oxidative protein modification in erythrocytes of patients with acute alcohol intoxication. Two groups of subjects were analyzed. The first one included 39 patients with acute alcohol intoxication. The second group consisted of 14 healthy subjects. Methods: In erythrocytes the activity of Cl–/HCО3– exchanger, the reactive protein carbonyl derivatives and membrane-bound hemoglobin concentration were measured. Results: Our results demonstrated strong alteration of the Cl–/HCО3– exchanger activity in erythrocytes of patients with acute alcohol intoxication. A delay in the beginning of hemolysis during incubation of erythrocytes in the ammonium medium was observed. The concentration of protein carbonyls in erythrocytes of patients significantly increased in comparison to the control ones. A decrease in the membrane-bounded hemoglobin was observed as well. Conclusions: These findings indicate that ethanol toxicity is manifested by alteration of oxidized protein concentration and Cl–/HCО3– exchanger activity in erythrocytes. It is hypothesized that oxidized proteins are implicated in modulation of the erythrocyte cell volume regulation.

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Oxidative stress modulates the organization of erythrocyte membrane cytoskeleton

Cited by 18 publications

References 0 publications

Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure

Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure

Synthesis andin vitrosafety assessment of magnetic bacterial cellulose with porcine aortic smooth muscle cells

The Oxidized proteins and activity of the Cl–/HCО3– exchanger in erythrocytes of patients with acute alcohol intoxication

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