Oxidative stress in alcohol-induced rat parotid sialadenosis

Campos, Sara Cristina Gonçalves; Moreira, Denise Aparecida Corrêa; Nunes, Terezinha D’Ávila e Silva; Colepicolo, Pio; Brigagão, Maı́sa Ribeiro Pereira Lima

doi:10.1016/j.archoralbio.2004.11.013

Cited by 20 publications

(11 citation statements)

References 39 publications

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“…In this study, the ethanol group showed conformity of response with the chronic heavy drinking model in the expression of cytokeratin and α -SMA [13], like the MDA levels [14] reported in previous studies. Binge consumption of ethanol, especially in adolescence and early adulthood, has been associated with alcoholism with the hypothesis that binge drinking may indicate a qualitatively similar imbalance but quantitatively lower when compared with a heavy chronic drinking model [25].…”

Section: Discussionmentioning

confidence: 63%

“…In contrast, neither period of ethanol exposure was able to cause changes in the EtOH group when compared to the control group or modify parotid or submandibular glands in terms of size or parenchyma volume, although it has been shown that heavy chronic consumption of ethanol can increase the size and cause atrophy of the parenchyma area of the parotid gland [13, 14]. This lack of alterations may be associated with the dosage of consumption of ethanol observed in a heavy model, but further studies are necessary to clarify this process.…”

Section: Discussionmentioning

confidence: 99%

“…Biochemical analysis, as revealed by oxidative stress, also shows changes that can occur in pathological situations, as in the case of EtOH chronic consumption, showing involvement of oxidative stress in parotid gland sialadenosis [14]. …”

Section: Introductionmentioning

confidence: 99%

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Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats

Fagundes

Fernandes

Paraense

et al. 2016

Oxidative Medicine and Cellular Longevity

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This study investigates morphological and biochemistry effects of binge ethanol consumption in parotid (PG) and submandibular (SG) salivary glands of rats from adolescence to adulthood. Female Wistar rats (n = 26) received ethanol at 3 g/kg/day (20% w/v) for 3 consecutive days/week from the 35th until the 62nd day of life. Animals were treated in two periods: 1 week (G1) and 4 weeks (G2), with a control (treated with distilled water) and an ethanol group to each period. In morphological analysis, morphometric and immunohistochemistry evaluation for smooth muscle actin (αSMA), cytokeratin-18 (CK-18), and vimentin (VIM) were made. Biochemical changes were analyzed by concentration of nitrites and levels of malondialdehyde (MDA). The difference between groups in each analysis was evaluated by Mann-Whitney U test or Student's t-test (p ≤ 0.05). PG showed, at one week of ethanol exposure, lower CK-18 and α-SMA expression, as well as MDA levels. After four weeks, lower CK-18 and higher MDA levels were observed in PG exposed to ethanol, in comparison to control group. SG showed lower α-SMA expression after 1 and 4 weeks of ethanol exposure as well as higher MDA levels after 1 week. Ethanol binge consumption during adolescence promotes tissue and biochemical changes with only one-week binge in acinar and myoepithelial PG cells.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

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Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats

Fagundes

Fernandes

Paraense

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…Oxidative stress is very harmful to the tissues and occurs in acute [50] as well as in chronic alcohol intoxication [51]. Oxidative stress is accompanied by the generation of reactive oxygen species (ROS) and increased activity of antioxidant enzymes e.g.…”

Section: Discussionmentioning

confidence: 99%

The effect of chronic alcohol intoxication and smoking on the activity of oral peroxidase

Waszkiewicz¹,

Zalewska²,

Szajda³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:Peroxidase is the most important antioxidant enzyme in saliva. Through peroxidation of thiocyanate in the presence of H 2 O 2 , peroxidase catalyses the formation of bacteriocidic compounds such as hypothiocyanate. The purpose of this study was to evaluate the effect of chronic alcohol intoxication and smoking on the activity of oral peroxidase (OPO). A total of 37 volunteers participated in the study. This cohort consisted of 17 male alcohol-dependent smoking patients after chronic alcohol intoxication (AS group, alcohol + smoking) (mean age: 42 years; range: 26-55) (100-700 g/day of alcohol; 10-20 cigarettes/day) and 20 control male social drinkers (CNS group, control non-smokers) with no history of alcohol abuse or smoking (mean age: 42 years; range: 30-53). Salivary peroxidase activity was measured by the colorimetric method. The differences between groups were evaluated using the Mann-Whitney U test. There was significantly higher activity of OPO (p = 0.00001) and significantly lower salivary flow (SF) (p = 0.007) in alcohol-dependent smokers after chronic alcohol intoxication compared to the control group. OPO activity significantly correlated with the number of days of alcohol intoxication, but not with smoking. Gingival index (GI) was significantly higher in smoking alcohol-dependent persons than in the control group, and correlated with OPO activity. The sensitivity of the OPO test was 70% in smoking alcoholics, while specificity was 95%. The increased activity of OPO suggests chronic oxidative stress is more likely due to ethanol action than to smoking. Smoking alcohol-dependent persons have a worse periodontal status than controls. OPO activity as a marker of chronic alcohol abuse may help in the diagnosis of alcoholism.

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“…In future studies, an alternative is the dissolution of vitamin E in another vehicle, such as corn oil (Domitrovic et al, 2008). Another possibility would be to use a lower concentration of Tween in the solution, such as 0.01%, that did not appear to be noxious (Campos et al, 2005). In this study, Tween 80 was chosen because: (i) it allows the preparation of a water-soluble mixture, which was suitable in order to improve gastrointestinal absorption and (ii) it was used in other studies (Krishnamurthy & Bieri, 1963;Ilavazhagan et al, 2001) without any adverse effects.…”

Section: Correlation Of Cell Proliferation and Oxidative Parametersmentioning

confidence: 99%

Exploring the mechanisms of alcohol-related damage in oral mucosa – is oxidative stress associated with the increase in cell proliferation in rat tongue epithelium?

Carrard

Pires

Mendez

et al. 2012

Pharmaceutical Biology

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Oxidative stress in alcohol-induced rat parotid sialadenosis

Cited by 20 publications

References 39 publications

Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats

Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats

The effect of chronic alcohol intoxication and smoking on the activity of oral peroxidase

Exploring the mechanisms of alcohol-related damage in oral mucosa – is oxidative stress associated with the increase in cell proliferation in rat tongue epithelium?

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