Oxidative stress, apoptosis and abnormal expression of apoptotic protein and gene and cell cycle arrest in the cecal tonsil of broilers induces by dietary methionine deficiency

Wu, Biao; Zhu, Maoqun; Ruan, Tao; Li, L.J.; Lyu, Yingnan; Wang, H.S.

doi:10.1016/j.rvsc.2018.10.009

Cited by 11 publications

(5 citation statements)

References 45 publications

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“…It is unclear if these BP terms are functioning as a result of a stressor in the CT. How Campylobacter activate oxidant stress in the CT of colonized poults is unknown. Methionine is a key limiting amino acid in poultry, and its deficiency produced oxidant stress in CT of chickens (57). Campylobacter are fastidious and some strains (e.g., C. jejuni NCTC 11168) require methionine for growth in a minimal medium (58), and rapidly consume it from medium (59).…”

Section: Host-response To Cck Colonizationmentioning

confidence: 99%

The Acute Host-Response of Turkeys Colonized With Campylobacter coli

et al. 2021

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Consumption of contaminated poultry products is one of the main sources of human campylobacteriosis, of which Campylobacter jejuni subsp. jejuni (C. jejuni) and C. coli are responsible for ~98% of the cases. In turkeys, the ceca are an important anatomical site where Campylobacter asymptomatically colonizes. We previously demonstrated that commercial turkey poults colonized by C. jejuni showed acute changes in cytokine gene expression profiles, and histological intestinal lesions at 2 days post-inoculation (dpi). Cecal tonsils (CT) are an important part of the gastrointestinal-associated lymphoid tissue that surveil material passing in and out of the ceca, and generate immune responses against intestinal pathogens. The CT immune response toward Campylobacter remains unknown. In this study, we generated a kanamycin-resistant C. coli construct (CcK) to facilitate its enumeration from cecal contents after experimental challenge. In vitro analysis of CcK demonstrated no changes in motility when compared to the parent isolate. Poults were inoculated by oral gavage with CcK (5 × 107 colony forming units) or sterile-media (mock-colonized), and euthanized at 1 and 3 dpi. At both time points, CcK was recovered from cecal contents, but not from the mock-colonized group. As a marker of acute inflammation, serum alpha-1 acid glycoprotein was significantly elevated at 3 dpi in CcK inoculated poults compared to mock-infected samples. Significant histological lesions were detected in cecal and CT tissues of CcK colonized poults at 1 and 3 dpi, respectively. RNAseq analysis identified 250 differentially expressed genes (DEG) in CT from CcK colonized poults at 3 dpi, of which 194 were upregulated and 56 were downregulated. From the DEG, 9 significantly enriched biological pathways were identified, including platelet aggregation, response to oxidative stress and negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway. These data suggest that C. coli induced an acute inflammatory response in the intestinal tract of poults, and that platelet aggregation and oxidative stress in the CT may affect the turkey's ability to resist Campylobacter colonization. These findings will help to develop and test Campylobacter mitigation strategies to promote food safety in commercial turkeys.

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Section: Host-response To Cck Colonizationmentioning

confidence: 99%

The Acute Host-Response of Turkeys Colonized With Campylobacter coli

et al. 2021

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“…The increased rate of apoptosis within avian lymphoid organs is a key feature of several metabolic and infectious diseases (Rauf, Khatri, Murgia, & Saif, 2012;B. Y. Wu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The increased rate of apoptosis within avian lymphoid organs is a key feature of several metabolic and infectious diseases (Rauf, Khatri, Murgia, & Saif, 2012; B. Y. Wu et al, 2018). Caspase‐3 is an enzyme with a well‐established role in initiating apoptotic cell death.…”

Section: Discussionmentioning

confidence: 99%

Structure of the Eurasian moorhen spleen: A comprehensive study using gross anatomy, light, and transmission electron microscopy

Abdellatif

2021

Microscopy Res & Technique

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The spleen is the largest secondary lymphoid organ with major roles in the removal of blood-borne antigens. The Eurasian moorhen is a wild aquatic bird that revealed the adaptation to harsh environmental conditions. Information regarding the structural features of moorhen's spleen is lacking. The present study aimed to describe the composition of moorhen's spleen using anatomical dissection, histology, histochemistry, immunohistochemistry, and transmission electron microscopy. The spleen appeared as a brownish red sickle-shaped organ close to the proventriculus and gizzard. The splenic capsule was very thin, and the trabeculae were poorly developed. The white pulp formed of the periarteriolar lymphoid sheath, lymphoid follicles, and periellipsoidal lymphatic sheath. The red pulp composed of sinusoids and cords and contained various types of blood cells. Blood vessels were observed within the splenic capsule and inside the parenchyma. Notably, penicillar capillaries (PCs) appeared branched and surrounded by welldeveloped ellipsoids. Direct connections were observed between PCs and splenic sinusoids suggesting a closed type of circulation. Ultrastructurally, intercellular spaces and vascular channels were evident in the wall of PCs. Ellipsoid-associated cells, lymphocytes, monocytes, and heterophils were observed within splenic ellipsoids. Ellipsoids were thus suggested as a crucial component of moorhen's spleen. Numerous MafB-immunoreactive (IR) macrophages were seen within the red pulp. Splenic cords contained the highest number of PHH3-IR cells, while CCASP3-IR cells were exclusive to follicles of the white pulp. In conclusion, the structure of moorhen's spleen revealed species-specific features.The findings of the present study could help to improve the immunity of domestic birds. Highlights• The Eurasian moorhen is a wild aquatic bird adapted to harsh environmental conditions with no known information about the cellular composition of its spleen.• The Eurasian moorhen's spleen revealed numerous penicillar capillaries surrounded by well-developed ellipsoids and appeared directly connected to the splenic sinusoids forming a closed type of circulation.• Immunohistochemical staining revealed site-specific staining for macrophages, proliferating, and apoptotic cells within moorhen's spleen.

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“…Both the control group and methionine deficiency diets were prepared from corn, soybeans, and wheat purchased from Jining, Shandong, and the feed formula was based on (Wu et al, 2018). The rest of the protein content, energy, vitamin, and trace element additions are added based on the nutritional standards of NRC (1994) for chickens.…”

Section: Methodsmentioning

confidence: 99%

Effects of Methionine Deficiency on Nutrient Composition, No, Nos Activity and Mrna Expression in Nf-κb Signal Pathway of the Liver and Kidney in Broiler

Mou,

Li,

et al. 2024

Braz. J. Poult. Sci.

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The study aimed to investigate the effect of methionine deficiency on conventional nutrient composition, NO and NOS activity in the liver and kidney, and expression of genes related to the NF-κB signal pathway. One hundred 1-day-old broilers were divided into control and methionine deficiency group, and provided with a standard diet. The methionine-deficient group was fed with a methionine-deficient diet for 42 days. Moisture content, protein content, and crude lipid content were determined by freeze drying, the Kjeldahl method, and the ether extraction method. The concentration of NO was measured using the nitrate reductase method, and the activity of NOS was measured using colorimetry. mRNA expression of related genes in the NF-κB signal pathway was detected by reverse transcription-polymerase chain reaction (RT-PCR). The results showed the contents of crude protein and crude lipid in the liver and kidney of the methionine-deficient group were significantly higher than those in the control group (p<0.05 or p<0.01). There was no significant change in dry matter (p>0.05). Methionine deficiency resulted in significantly increased NO concentration and total nitric oxide synthase (TNOS) activities in the liver and kidney (p<0.01). In contrast, cNOS and inducible iNOS activities significantly decreased (p<0.01). The expression of NF-κB, TNF-α, IFN-γ, IL-1, and IL-6 mRNA, which are related to the NF-κB signal pathway of the methioninedeficient group, were significantly higher than those in the control group (p<0.05 or p<0.01). It is concluded that long-term feeding diets lacking methionine will lead to changes in nutrient composition and inflammatory injury of the liver and kidney.

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Oxidative stress, apoptosis and abnormal expression of apoptotic protein and gene and cell cycle arrest in the cecal tonsil of broilers induces by dietary methionine deficiency

Cited by 11 publications

References 45 publications

The Acute Host-Response of Turkeys Colonized With Campylobacter coli

The Acute Host-Response of Turkeys Colonized With Campylobacter coli

Structure of the Eurasian moorhen spleen: A comprehensive study using gross anatomy, light, and transmission electron microscopy

Effects of Methionine Deficiency on Nutrient Composition, No, Nos Activity and Mrna Expression in Nf-κb Signal Pathway of the Liver and Kidney in Broiler

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