Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights into ageing, apoptosis and diseases

Costa, Vítor Santos; Moradas‐Ferreira, Pedro

doi:10.1016/s0098-2997(01)00012-7

Cited by 232 publications

(191 citation statements)

References 127 publications

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“…These changes contribute to the elimination of an important percentage of odontoblasts by apoptosis at the beginning of secondary dentin formation. This is in accordance with numerous studies showing that aging is accompanied by a general decline of physiological function and significant increase of apoptosis [3,23,27].…”

Section: Apoptosis In Intact Human Teeth At the Beginning Of Secondarsupporting

confidence: 93%

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Apoptosis in developmental and repair-related human tooth remodeling: A view from the inside

Mitsiadis

Bari

About

2008

Experimental Cell Research

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Section: Apoptosis In Intact Human Teeth At the Beginning Of Secondarsupporting

confidence: 93%

“…Genetic evidence has identified both positive and negative regulators of apoptosis [3,4]. Initiators of apoptosis include, between others, ultraviolet irradiation and deprivation of survival factors such as cytokines.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis in developmental and repair-related human tooth remodeling: A view from the inside

Mitsiadis

Bari

About

2008

Experimental Cell Research

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“…Antioxidant defenses and stress proteins play critical roles in cellular protection against oxidative damages (Costa and Moradas-Ferreira, 2001). Aiming to identify genes differentially expressed in Isc1p-deficient cells, we initially analyzed both constitutive and H 2 O 2 -induced mRNA levels of some antioxidant defense genes (SOD1, Cu,Zn-superoxide dismutase; SOD2, Mn-superoxide dismutase; CTT1, cytosolic catalase) and heat-shock proteins (HSP26 and SSA4, a HSP70 family member).…”

Section: Transcriptome Analysis In Isc1⌬ Mutant Cellsmentioning

confidence: 99%

Isc1p Plays a Key Role in Hydrogen Peroxide Resistance and Chronological Lifespan through Modulation of Iron Levels and Apoptosis

Almeida

Marques

Mojžita

et al. 2008

MBoC

107

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The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p resulted in a higher sensitivity to hydrogen peroxide that was associated with an increase in oxidative stress markers, namely intracellular oxidation, protein carbonylation, and lipid peroxidation. Microarray analysis showed that Isc1p deficiency up-regulated the iron regulon leading to increased levels of iron, which is known to catalyze the production of the highly reactive hydroxyl radicals via the Fenton reaction. In agreement, iron chelation suppressed hydrogen peroxide sensitivity of isc1⌬ mutants. Cells lacking Isc1p also displayed a shortened chronological lifespan associated with oxidative stress markers and aging of parental cells was correlated with a decrease in Isc1p activity. The analysis of DNA fragmentation and caspase-like activity showed that Isc1p deficiency increased apoptotic cell death associated with oxidative stress and aging. Furthermore, deletion of Yca1p metacaspase suppressed the oxidative stress sensitivity and premature aging phenotypes of isc1⌬ mutants. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis.

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“…Aerobic organisms have evolved antioxidant enzymatic systems to protect against reactive oxygen species (e.g., superoxide, hydroxyl radical, and hydrogen peroxide) generated as byproducts of metabolism or resulting from environmental insults [1,2]. Significant sources of endogenous reactive oxygen species are mitochondrial respiration, in which incomplete reduction of oxygen results in the generation of superoxide anion, and peroxisomal β-oxidation, in which hydrogen peroxide is a stoichiometric product of the first enzymatic reaction.…”

Section: Introductionmentioning

confidence: 99%

Changes in disulfide bond content of proteins in a yeast strain lacking major sources of NADPH

Minard

Carroll

Weintraub

et al. 2007

Free Radical Biology and Medicine

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A yeast mutant lacking the two major cytosolic sources of NADPH, glucose-6-phosphate dehydrogenase (Zwf1p) and NADP + -specific isocitrate dehydrogenase (Idp2p), has been demonstrated to lose viability when shifted to medium with acetate or oleate as the carbon source. This loss in viability was found to correlate with an accumulation of endogenous oxidative byproducts of respiration and peroxisomal β-oxidation. To assess effects on cellular protein of endogenous versus exogenous oxidative stress, a proteomics approach was used to compare disulfide bondcontaining proteins in the idp2Δzwf1Δ strain following shifts to acetate and oleate media with those in the parental strain following similar shifts to media containing hydrogen peroxide. Among prominent disulfide bond-containing proteins were several with known antioxidant functions. These and several other proteins were detected as multiple electrophoretic isoforms, with some isoforms containing disulfide bonds under all conditions and other isoforms exhibiting a redox-sensitive content of disulfide bonds, i.e., in the idp2Δzwf1Δ strain and in the hydrogen peroxide-challenged parental strain. The disulfide bond content of some isoforms of these proteins was also elevated in the parental strain grown on glucose, possibly suggesting a redirection of NADPH reducing equivalents to support rapid growth. Further examination of protein carbonylation in the idp2Δzwf1Δ strain shifted to oleate medium also led to identification of common and unique protein targets of endogenous oxidative stress.

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Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights into ageing, apoptosis and diseases

Cited by 232 publications

References 127 publications

Apoptosis in developmental and repair-related human tooth remodeling: A view from the inside

Apoptosis in developmental and repair-related human tooth remodeling: A view from the inside

Isc1p Plays a Key Role in Hydrogen Peroxide Resistance and Chronological Lifespan through Modulation of Iron Levels and Apoptosis

Changes in disulfide bond content of proteins in a yeast strain lacking major sources of NADPH

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