“…SIRT3 is considered a crucial protein against oxidative stress, since it deacetylates and activates several proteins related to mitochondrial function and antioxidant enzymes (Alhazzazi, Kamarajan, Verdin, & Kapila, ; Oliver, Roca, & Sastre‐Serra, ). In this regard, we observed an increase in ROS levels and ROS production after SIRT3 knockdown, as has been widely described before (Finley & Haigis, ; Finley et al, ; Kong et al, ; Park, Choi, Gurunathan, & Kim, ; Pons, Sastre‐Serra, Oliver, & Roca, ). This increase has been attributed to the hyperacetylation, and thus inactivation, of SIRT3 targets, mainly MnSOD, which is one of the most important antioxidant enzymes of the cell (Ozden et al, ; Vassilopoulos, Parisiadou, Yan, & Gius, ; Zou et al, ).…”