Oxidative stress and hepatotoxicity in rats induced by poisonous pufferfish (Lagocephalus lagocephalus) meat

Abstract

ABSTRACT:This study was undertaken to evaluate the effect of pufferfish (Lagocephalus lagocephalus) meat poisoning on hepatic functions of Wistar rats. For this purpose, groups of rats (Lcr, Lcu+b and Lcu-b) received diet supplemented with 10% of raw or cooked meat, respectively, with or without cooking water of L. lagocephalus while groups Mcr and Mcu+b received diet supplemented with 10% of raw or cooked meat of Liza aurata, which were used as a negative control. In Lcu+b group, ALT, AST and ALP rates (hepatic enzyme markers) decreased after two months of treatment, indicating liver damage. We also observed an increase of 54 and 65% of thiobarbituric acid reactive substances (TBARS) in their livers respectively 48 hours and two months after treatment compared to controls. The catalase (CAT) activity in group Lcu+b decreased (p < 0.05) after two periods of treatment, whereas metallothionein (MT) level significantly increased and decreased, respectively after 48 hours and two months. In fact, in the histological analysis of the livers from Lcu+b treated group, we observed an increase in vacuolisation, necrosis, hepatocytes ballooning and sinusoids dilation. These results indicate that L. lagocephalus meat cooked with water produces hepatotoxicity and oxidative damage.

Risks for public health related to the presence of tetrodotoxin (TTX) and TTX analogues in marine bivalves and gastropods

“…injection of extract, homogenate (Zaki et al, 2001;Saoudi et al, 2011) or oral administration (Saoudi et al, 2009) of meat obtained from different (Zaki et al, 2001), decrease of serum enzyme activity (i.e. aldolase (ALS), alanine aminotransferase (ALT), alkaline phosphatase (ALP), y-glutamyl transpeptidase (GTP) and lactate dehydrogenase (LDH)), hepatic damage (increased lipid peroxidation, focal necrosis, hepatic chord, infiltration of lymphocytes, congestion in central veins and sinusoids; Saoudi et al, 2009Saoudi et al, , 2011 were observed. No information on the amount of TTX or the presence of analogues was provided.…”

“…Acute toxicity studies with TTX from extracts and pufferfish meat Some studies describe the toxicity induced by i.p. injection of extract, homogenate (Zaki et al, 2001;Saoudi et al, 2011) or oral administration (Saoudi et al, 2009) of meat obtained from different types of pufferfish (Lagocephalus sceleratus and Lagocephalus lagocephalus) as a source of TTX.…”

“…Some studies describe the toxicity induced by i.p. injection of extract, homogenate (Zaki et al., ; Saoudi et al., ) or oral administration (Saoudi et al., ) of meat obtained from different types of pufferfish ( Lagocephalus sceleratus and Lagocephalus lagocephalus ) as a source of TTX. Reversible increase in different neurotransmitters (serotonin, acetylcholine, histamine and noradrenaline) (Zaki et al., ), decrease of serum enzyme activity (i.e.…”

Assessing the Quality of Household and Drinking Water in Tongi Industrial Zone of Bangladesh and Its Toxicological Impact on Healthy Sprague Dawley Rats

“…The co-exposure of metal/ metalloid mixtures of heavy metals in household and drinking water produce severe effects at both relatively high and low dose levels [31,[41][42][43][44][45][46]. This study is a brief reflection of the toxicological effects of industrialization on water resources, as well as the pharmacological effects of contaminated household and drinking water on animal model of an industrialized area in a developing country.…”

Extracted tetrodotoxin from puffer fish Lagocephalus lagocephalus induced hepatotoxicity and nephrotoxicity to Wistar rats

“…In our previous study (Saoudi et al, 2007), the toxicity risk of L. lagocephalus showed a disturbance in physiological and biochemical parameters in Wistar rats nourished with L. lagocephalus when compared with rats fed the diet contained a non toxic fish, the mule Liza aurata. The diet containing meat cooked with L. lagocephalus cooking water induced hepatotoxicity and oxidative stress in rats after 48 h and 2 months of treatment (Saoudi et al, 2009).…”