Oxidative DNA damage and multi-organ pathologies in male mice subchronically treated with aflatoxin B1

Zhou, Hongyuan; Wang, Jiaman; Ma, Liang; Chen, Lu; Guo, Ting; Zhang, Yuhao; Dai, Hongjie; Yu, Yong

doi:10.1016/j.ecoenv.2019.109697

Cited by 15 publications

(7 citation statements)

References 40 publications

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“…It is well-established that AFB1 causes DNA damage [ 95 ], and the present induction of molecular networks linked to tumor suppressor P53 most likely aims to protect cells against the accumulation of deleterious mutations. Similarly, the activation of P53-regulated signaling pathways has been previously reported either in vivo in mice sub-chronically treated with AFB1 [ 96 ] or in vitro in human colorectal carcinoma cells treated for 24 h with 0.1, 1, 5, and 10 μM AFB1 [ 58 ].…”

Section: Discussionmentioning

confidence: 72%

Insights into Aflatoxin B1 Toxicity in Cattle: An In Vitro Whole-Transcriptomic Approach

Pauletto

Tolosi²,

Giantin³

et al. 2020

Toxins

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Aflatoxins, and particularly aflatoxin B1 (AFB1), are toxic mycotoxins to humans and farm animal species, resulting in acute and chronic toxicities. At present, AFB1 is still considered a global concern with negative impacts on health, the economy, and social life. In farm animals, exposure to AFB1-contaminated feed may cause several untoward effects, liver damage being one of the most devastating ones. In the present study, we assessed in vitro the transcriptional changes caused by AFB1 in a bovine fetal hepatocyte-derived cell line (BFH12). To boost the cellular response to AFB1, cells were pre-treated with the co-planar PCB 3,3′,4,4′,5-pentachlorobiphenyl (PCB126), a known aryl hydrocarbon receptor agonist. Three experimental groups were considered: cells exposed to the vehicle only, to PCB126, and to PCB126 and AFB1. A total of nine RNA-seq libraries (three replicates/group) were constructed and sequenced. The differential expression analysis showed that PCB126 induced only small transcriptional changes. On the contrary, AFB1 deeply affected the cell transcriptome, the majority of significant genes being associated with cancer, cellular damage and apoptosis, inflammation, bioactivation, and detoxification pathways. Investigating mRNA perturbations induced by AFB1 in cattle BFH12 cells will help us to better understand AFB1 toxicodynamics in this susceptible and economically important food-producing species.

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Section: Discussionmentioning

confidence: 72%

Insights into Aflatoxin B1 Toxicity in Cattle: An In Vitro Whole-Transcriptomic Approach

Pauletto

Tolosi²,

Giantin³

et al. 2020

Toxins

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“…A likely explanation of this association is not yet identified, though consumption of AF-contaminated foods and feeds were reported to cause diverse degrees of liver injury comprising development of fatty cysts, fibrosis, and cirrhosis among humans and animals [23][24][25][26][27]. However, several lines of evidence support oxidative stress as a key factor in AF induced initiation and progression of liver cirrhosis [28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Aflatoxins as a risk factor for liver cirrhosis: a systematic review and meta-analysis

Mekuria

Routledge

Gong

et al. 2020

BMC Pharmacol Toxicol

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Background: Liver cirrhosis is characterized by fibrosis and nodule formation in the liver, due to a chronic injury, and subsequent alteration of the normal architecture of the liver. Even though there is a huge effort to elucidate the possible etiologic factors of liver cirrhosis, a significant number of cases are cryptogenic, especially in Sub Saharan Africa, where there is a high burden of aflatoxin exposure. Aflatoxins are known to cause hepatocellular carcinoma, which share similar etiologic factors with liver cirrhosis. This study aimed to assess the association between aflatoxin exposure and the risk of liver cirrhosis. Methods: Relevant studies were identified through systematic searches conducted in Ovid MEDLINE, PubMed and Google Scholar. Also, by searching the references of retrieved articles. The abstracts and full text were screened for eligibility and the risk of bias was assessed for each study using Joanna Briggs Institute (JBI) critical appraisal checklist for observational studies. The extracted data from included studies using Microsoft Excel were exported to Stata software version 15.0 for analyses. The overall pooled estimation of outcomes was calculated using a randomeffects model of DerSimonian-Laird method at a 95% confidence level. The heterogeneity of studies was determined using I2 statistics. The presence of publication bias between studies was evaluated using the Begg's and Egger's tests and funnel plot. The protocol of this systematic review and meta-analysis was registered in the Prospero database with reference number ID: CRD42019148481. Results: A total of 5 studies published between the years 2005 and 2018 that met the pre-defined inclusion and exclusion criteria were included. The meta-analysis showed that a significant increase in the risk of liver cirrhosis is associated with aflatoxin exposure (unadjusted pooled odds ratio (OR) = 3.35, 95% CI: 2.74-4.10, p = 0.000; I 2 = 88.3%, p = 0.000; adjusted OR = 2.5, 95% CI: 1.84-3.39, p = 0.000; I 2 = 0%, p = 0.429). Conclusions: The present meta-analysis suggests that aflatoxin exposure is associated with a higher risk of liver cirrhosis.

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“…Some toxic effects of AFs have been observed in the most recent literature. Hepatotoxic effect of AFB 1 has been demonstrated in vivo on mice [110,111], rats [112], rabbits [113], and broiler chickens [114]. The nephrotoxic effect of AFB 1 has been reported in vivo on broiler chickens [114] and rats [112].…”

Section: Recent Data On Aflatoxin Toxicitymentioning

confidence: 98%

“…The immunosuppression of AFB 1 has been demonstrated in vitro on swine alveolar macrophages [116]. Reproductive toxicity of AFB 1 has been demonstrated in vivo on mice [110]. Pulmonary toxicity of AFB 1 has been observed in vivo on male albino rats [117].…”

Section: Recent Data On Aflatoxin Toxicitymentioning

confidence: 99%

“…Gastrointestinal toxicity of AFB 1 has been described in vivo on rats [118], pigs [119], and chickens [120]. The genotoxic effect has been observed in vivo on mice [110]. Cytotoxic and/or genotoxic effects of AFB 1 have been reported in vitro on the leghorn male hepatoma (LMH) cell line [121], the liver hepatocellular carcinoma (HepG2) cell line [122], buffalo rat liver (BRL-3A) cells [123], bovine mammary epithelial (BME) cells [124], and the human keratinocyte (HaCaT) cell line [125].…”

Section: Recent Data On Aflatoxin Toxicitymentioning

confidence: 99%

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Aflatoxins: History, Significant Milestones, Recent Data on Their Toxicity and Ways to Mitigation

Pickova

Ostrý

Toman

et al. 2021

Toxins

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In the early 1960s the discovery of aflatoxins began when a total of 100,000 turkey poults died by hitherto unknown turkey “X” disease in England. The disease was associated with Brazilian groundnut meal affected by Aspergillus flavus. The toxin was named Aspergillus flavus toxin—aflatoxin. From the point of view of agriculture, aflatoxins show the utmost importance. Until now, a total of 20 aflatoxins have been described, with B1, B2, G1, and G2 aflatoxins being the most significant. Contamination by aflatoxins is a global health problem. Aflatoxins pose acutely toxic, teratogenic, immunosuppressive, carcinogenic, and teratogenic effects. Besides food insecurity and human health, aflatoxins affect humanity at different levels, such as social, economical, and political. Great emphasis is placed on aflatoxin mitigation using biocontrol methods. Thus, this review is focused on aflatoxins in terms of historical development, the principal milestones of aflatoxin research, and recent data on their toxicity and different ways of mitigation.

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Oxidative DNA damage and multi-organ pathologies in male mice subchronically treated with aflatoxin B1

Cited by 15 publications

References 40 publications

Insights into Aflatoxin B1 Toxicity in Cattle: An In Vitro Whole-Transcriptomic Approach

Insights into Aflatoxin B1 Toxicity in Cattle: An In Vitro Whole-Transcriptomic Approach

Aflatoxins as a risk factor for liver cirrhosis: a systematic review and meta-analysis

Aflatoxins: History, Significant Milestones, Recent Data on Their Toxicity and Ways to Mitigation

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