Oxidative deoxyribonucleic acid damage in sperm has a negative impact on clinical pregnancy rate in intrauterine insemination but not intracytoplasmic sperm injection cycles

Thomson, Laura Kelly; Zieschang, Julie-Anne; Clark, Anne M.

doi:10.1016/j.fertnstert.2011.07.356

Cited by 54 publications

(40 citation statements)

References 37 publications

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“…Such stress is not only involved in the origins of defective sperm function (Aitken & Clarkson 1987, Aitken & Curry 2011), but also a major mediator of DNA damage in the male germ line (De Iuliis et al 2009, Thomson et al 2011, resulting in poor fertilisation rates, an increased risk of spontaneous abortion and morbidity in the offspring (Aitken & Curry 2011). Because the molecular structure of sperm chromatin approaches the physical limits of compaction, spermatozoa have a very limited capacity for DNA repair (Ward & Coffey 1991).…”

Section: Introductionmentioning

confidence: 99%

The senescence-accelerated mouse prone 8 as a model for oxidative stress and impaired DNA repair in the male germ line

Smith¹,

Iuliis²,

Lord³

et al. 2013

Reproduction

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The discovery of a truncated base excision repair pathway in human spermatozoa mediated by OGG1 has raised questions regarding the effect of mutations in critical DNA repair genes on the integrity of the paternal genome. The senescence-accelerated mouse prone 8 (SAMP8) is a mouse model containing a suite of naturally occurring mutations resulting in an accelerated senescence phenotype largely mediated by oxidative stress, which is further enhanced by a mutation in the Ogg1 gene, greatly reducing the ability of the enzyme to excise 8-hydroxy,2 0 -deoxyguanosine (8OHdG) adducts. An analysis of the reproductive phenotype of the SAMP8 males revealed a high level of DNA damage in caudal epididymal spermatozoa as measured by the alkaline Comet assay. Furthermore, these lesions were confirmed to be oxidative in nature, as demonstrated by significant increases in 8OHdG adduct formation in the SAMP8 testicular tissue (P!0.05) as well as in mature spermatozoa (P!0.001) relative to a control strain (SAMR1). Despite this high level of oxidative DNA damage in spermatozoa, reactive oxygen species generation was not elevated and motility of spermatozoa was found to be similar to that for the control strain with the exception of progressive motility, which exhibited a slight but significant decline with advancing age (P!0.05). When challenged with Fenton reagents (H 2 O 2 and Fe 2C ), the SAMP8 spermatozoa demonstrated a highly increased susceptibility to formation of 8OHdG adducts compared with the controls (P!0.001). These data highlight the role of oxidative stress and OGG1-dependent base excision repair mechanisms in defining the genetic integrity of mammalian spermatozoa.

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Section: Introductionmentioning

confidence: 99%

The senescence-accelerated mouse prone 8 as a model for oxidative stress and impaired DNA repair in the male germ line

Smith¹,

Iuliis²,

Lord³

et al. 2013

Reproduction

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“…Nevertheless, other authors describe the sperm DNA's damage as a useful factor to be identified in the assessment of IUI (Intrauterine Insemination) success rates, stating that it is not a factor with a negative impact on ICSI treatments. So, they assert that the human sperm DNA has a significant value in estimating the chances of achieving a clinic pregnancy after an IUI, but they confirm that DNA fragmentation does not seem to influence in the chances of achieving a successful result after an ICSI [3].…”

Section: Annexin V-bindingmentioning

confidence: 99%

“…In other words, the spermatozoa passing through the magnetic columns without being retained will prove to have an unharmed DNA and, therefore, they will be eligible to be used. Consequently, specific criteria should be adopted: the initial fraction should not be so severe at the time of indicating a future IUI, that is to say, it must show some sperm parameters which allow us to apply this technique because, if the sample reveals borderline parameters, we run the risk of collecting a very small negative fraction [3]. Anyway, the previous treatment with orally administered antioxidants will not be in vain because performing an ICSI treatment with an improved sperm sample will also improve the chances of getting pregnant [17].…”

Section: Annexin V-bindingmentioning

confidence: 99%

Annexin V-binding and assisted reproduction technologies

Denaday¹

2013

OJMIP

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The objective is to describe the impact annexin Vbinding technique has on couples diagnosed with male infertility and how to optimize sperm parameters so that this technique contributes to simplify the assisted reproductive treatment that will be prescribed [1-4]. Empirical studies and expert consensus related to the physiological and medical aspects of the use of annexin V-binding which were presented in this study were identified in articles through bibliographical research.

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“…Thomson ve ark. 53 IUI siklusunda TUNEL yöntemi kullanarak ve biyokimyasal belirteçler kullanarak yap›lan DNA fragmantasyonu de¤erlendirilmesinin baflar›y› öngörücü etkisi saptanm›flt›r (15) .…”

Section: Anilin Mavisi (Aniline Blue)unclassified