Oxidative damage lipid peroxidation in the kidney of choline-deficient rats

Abstract

Phosphatidylcholine is the most abundant phospholipid constituent of cell membranes and choline is a quaternary amine required for phosphatidylcholine synthesis. The impairment of membrane functions is considered as an indication of oxidative damage. In order to kinetically analyze the time course of the pathogenesis of renal necrosis following to choline deficiency in weanling rats, we determined markers of membrane lipid peroxidation (thiobarbituric acid reactive substances; TBARS and hydroperoxide-induced chemiluminescence (BOOH-CL) ) and studied the histopathological damage. Plasma TBARS (t(1/2) = 2.5 days) was an early indicator of systemic oxidative stress, likely involving liver and kidney. The levels of TBARS an BOOH-CL increased by 80% and by 183%, respectively, in kidney homogenates with t(1/2) = 1.5 days and 4 days, respectively. The levels of BOOH-CL were statistically higher in rats fed a choline-deficient diet at day 6, in a mixture of membranes (from plasmatic, smooth and rough endoplasmic reticulum and Golgi), in mitochondrial membranes and in lysosomal membranes. The results indicate that choline deficiency produces oxidative damage in kidney subcellular membranes. Necrosis involved mainly convoluted tubules and appeared with a t(1/2) = 5.5 days. An increase in the production of reactive oxygen species, triggered by NADH overproduction in the mitochondrial dysfunction associated with choline deficiency appears as one of the pathogenic mechanism of mitochondrial and cellular oxidative damage in choline-deficiency.

Evaluation of the protective role of quercetin and lecithin on Ifosfamide neurotoxicity in rats

“…Furthermore, phosphotidylcholine decreases severe sepsis in rat model, in liver, heart and lung MDA levels (Demirbilek et al, 2004). Although PC act as a choline donor, and could inhibit an increase in oxidative damage caused by choline-deficiency (Ossani et al, 2007) it failed to restore serum cholinesterase in present study. The molecular and cell signal mechanisms may be responsible for this effect and it was unclear.…”

Choline attenuates immune inflammation and suppresses oxidative stress in patients with asthma

“…In the present study, choline treatment significantly reduced isoprostanes levels compared to baseline and pharmacotherapy post-treatment. Choline deficiency activates production of reactive oxygen species (ROS) as one of the pathogenic mechanism of mitochondrial and oxidative damage (Ossani et al 2007). Mice fed with cholinedeficient diet showed increase in oxidative stress and altered antioxidants defenses (Grattagliano et al 2000;Yoshida et al 2006).…”

Choline supplementation reduces oxidative stress in mouse model of allergic airway disease

“…They were challenged with 2AE5% aerosolized OVA (w ⁄ v in saline) in a plexiglass chamber using nebulizer (Omron, Tokyo, Japan) for 30 min on days 25-27. Mice were treated once daily just after immunisation till last challenge (on days [14][15][16][17][18][19][20][21][22][23][24][25][26][27] with 100 ll saline (group 2), 1 mg kg -1 of choline in 100 ll saline by oral gavage (group 3), 1 mg kg -1 of choline in 50 ll saline intranasal (i.n.) (group 4) and 100 mg kg -1 of a-lipoic acid by oral gavage (group 5).…”