Oxidation of Triacyiglycerol by Laccase-Natural Mediator and Laccase-Mediator Derived from Natural Compound Systems.

Saba, Hayato; Nakano, Masuo

doi:10.5650/jos.52.159

Cited by 4 publications

(3 citation statements)

References 17 publications

(32 reference statements)

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“…However, as laccase is an oxidoreductase, previous experimental reports have shown that laccase reduces the oxidative stability of lipids by oxidation of phenols and PUFA, resulting in the oxidative loss of lipids and PUFA. [ 25,26 ]…”

Section: Resultsmentioning

confidence: 99%

Response Surface Optimization of Enzyme Pretreatment Improves Yield of Ethanol‐Extracted Lipids from Nannochloropsis oceanica

Zhao

Zhang

et al. 2022

Euro J Lipid Sci & Tech

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Nannochloropsis oceanica are marine microalgae that are rich in eicosapentaenoic acid (EPA); in the future, they may become one of the most effective sources of EPA. The cell walls of N. oceanica contain a three‐layered dense structure called algaenan, which hinders the extraction of intracellular lipids. Here, the main variables for the enzyme pretreatment of N. oceanica are optimized using response surface methodology. Under the optimal variables for enzyme pretreatment, the lipid yield and EPA content are increased to 26.9% and 20.7 g/100 g, respectively. Furthermore, lipidomics analysis is carried out utilizing ultra‐high performance liquid‐quadrupole‐time‐of‐flight mass spectrometry to identify the obtained lipids. After enzyme treatment, the betaine lipids (BL) content significantly increases, probably due to laccase and cellulase promoting the release of BL from membrane structure. In comparison, the hydrolysis of neutral lipids, glycolipids, and phospholipids during the enzyme treatment reduces their respective contents. The results of this study suggest that using compound enzymes to pretreat N. oceanica can effectively increase lipids extraction yields by ethanol and improve the EPA contents in lipids. Practical applications: This study indicates that enzyme pretreatment can promote the extraction of lipids, especially lipids with EPA, from N. oceanica or other marine microalgae.

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Section: Resultsmentioning

confidence: 99%

Response Surface Optimization of Enzyme Pretreatment Improves Yield of Ethanol‐Extracted Lipids from Nannochloropsis oceanica

Zhao

Zhang

et al. 2022

Euro J Lipid Sci & Tech

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“…Johannes and Majcherczyk succeeded in the degradation of polycyclic aromatic hydrocarbons by the laccase/mediator system using natural mediators (22). Saba and Nakano also reported that HBA and HBA derivatives were effective in oxidizing triacylglycerol as natural mediators (23). We investigated the degradation of dymron by the laccase/mediator system using natural mediators ( Figure 10).…”

Section: Screening Of Enzymes Capable Of Dymron Degradationmentioning

confidence: 99%

Laccase-Mediated Oxidative Degradation of the Herbicide Dymron

et al. 2006

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The present study reports the successful and effective degradation of the persistent herbicide dymron catalyzed by the oxidative enzyme laccase in the presence of a reaction mediator (a laccase/mediator system). Using 2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the mediator, over 90% of dymron was degraded within 24 h, while the half-life of dymron is 50 days in soil. The results suggested that oxidation of dymron resulted in the production of decomposed compounds with a single aromatic ring. We also found that edible surfactants and a dishwashing detergent were useful to solubilize dymron in an aqueous solution and did not inhibit the oxidative degradation. Degradation proceeded at acidic pH and in a broad range of temperatures (303-353 K). The use of natural mediators also allowed the oxidative degradation of dymron to some extent. In conclusion, we propose the possible use of a laccase/mediator system for the treatment of soils and drainwater contaminated with herbicides.

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“…The oxidation of unsaturated fatty acids and triglycerols by the laccase-mediator systems has already been investigated by different working groups. [24][25][26][27][28] The best results were achieved with 1-hydroxybenzotriazole (HBT) and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as mediators.…”

Section: Introductionmentioning

confidence: 99%

Banning toxic heavy-metal catalysts from paints: enzymatic cross-linking of alkyd resins

et al. 2013

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Alkyd resins are polyesters containing unsaturated fatty acids that are used as binding agents in paints and coatings. Chemical drying of these polyesters is based on heavy metal catalyzed cross-linking of the unsaturated fatty acid moieties. Among the heavy-metal catalysts, cobalt complexes are the most effective, yet they have been proven to be carcinogenic. Therefore, strategies to replace the cobalt-based catalyst by environmentally friendlier and less toxic alternatives are under development. Here, we demonstrate for the first time that a laccase-mediator system can effectively replace the heavy-metal catalyst and cross-link alkyd resins. Interestingly, the biocatalytic reaction does not only work in aqueous media, but also in a solid film, where enzyme diffusion is limited. Within the catalytic cycle, the mediator oxidizes the alkyd resin and is regenerated by the laccase, which is uniformly distributed within the drying film as evidenced by confocal laser scanning microscopy. During gradual build-up of molecular weight, there is a concomitant decrease of the oxygen content in the film. A new optical sensor to follow oxygen consumption during the cross-linking reaction was developed and validated with state of the art techniques. A remarkable feature is the low sample amount required, which allows faster screening of new catalysts.

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Oxidation of Triacyiglycerol by Laccase-Natural Mediator and Laccase-Mediator Derived from Natural Compound Systems.

Cited by 4 publications

References 17 publications

Response Surface Optimization of Enzyme Pretreatment Improves Yield of Ethanol‐Extracted Lipids from Nannochloropsis oceanica

Response Surface Optimization of Enzyme Pretreatment Improves Yield of Ethanol‐Extracted Lipids from Nannochloropsis oceanica

Laccase-Mediated Oxidative Degradation of the Herbicide Dymron

Banning toxic heavy-metal catalysts from paints: enzymatic cross-linking of alkyd resins

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