Oxidants-Antioxidants Profile in the Breast Cancer Cell Line MCF-7

Abstract

Reactive oxygen species (ROS) have various biological effects and they are non-linear in characteristic. In high oxidative stress, they may cause cytotoxicity, inhibit cell proliferation, and induce cell death in the form of apoptosis/necrosis; while in low or medium oxidative stress, ROS may cause DNA damage, cell mutation, inflammation, cell proliferation, and eventually they may induce carcinogenesis. Antioxidants are compounds with the ability to reduce ROS. Cell line MCF-7 is one of the breast cancer cell lines that is known to have small amount of antioxidant MnSOD compared to the other cell lines. Low antioxidant MnSOD level in breast cancer cell line MCF-7 leads to low concentration of hydrogen peroxide, because antioxidant MnSOD will convert radical superoxide to hydrogen peroxide. The aim of this research was to analyze oxidants and antioxidants profile in breast cancer cell line MCF-7 and their relationship with cell number. Observations were conducted for 5 days. The cell number was counted with tryphan blue method and haematometer. The concentration of radical superoxide was measured with DHE staining using LSCM tipe Olympus Fluoview FV 1000-Ver 1.7. MnSOD activity, hydrogen peroxide concentration, and catalase activity were measured with ELISA. The results showed that the longer of observation, the greater concentration of oxidants and MnSOD activity, but there was no change in catalase activity. Conclusion the increase in cancer cells number is influenced by radical superoxide.

Osteoblast-Derived Paracrine and Juxtacrine Signals Protect Disseminated Breast Cancer Cells from Stress

“…Interestingly, MCF7 cells (ER pos ) showed an enhanced level of cell death induced by oxidative stress. This finding is in agreement with reports that MCF7 cells (ER pos ) are sensitive to oxidative stress which arises from their low endogenous levels of some antioxidants [ 48 ], which is further compounded by estrogen signaling [ 49 ]. In addition, like the majority of breast cancers, MCF7 cells express both ER and wildtype, functional p53, which renders MCF7 cells sensitive to cell death induced by oxidative stress [ 49 , 50 , 51 ].…”

Comparative Effects Between the Commercial and Homemade Curcuma on HCT116 Adenocarcinoma Cell Line

“…Our results show that cur T display the strongest DDPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging activity comparing it to cur C. The lower IC 50 corresponds to the highest radical scavenging effectiveness observed with cur T. A lot of studies showed an important antioxidant effect of curcuma [14] and others concluded that this effect is due to Curcumin [13] but Curcuma contains other molecules that have the same effect [15].…”

The Antioxidant and Antiproliferative Activities of 1,2,3-Triazolyl-L-Ascorbic Acid Derivatives

“…The observed differences in compound 4e activity on MCF-7 tumor cells in comparison with normal fibroblasts HFF-1 may be due to the specific metabolic status of tumor cells, in which case an antioxidant compound with ROS scavenging properties, such as L-ASA, may have a cytotoxic effect; i.e., through a pro-oxidant effect on cancer cells associated with increase of ROS in tumor cells [55]. Particularly in MCF-7 cells, a small amount of an important cellular antioxidant enzyme manganese-dependent superoxide dismutase (MnSOD) was found in comparison with other cell lines [56]. It is therefore, possible that the compound 4e exerts a pro-oxidant activity in MCF-7 cells, which elevates the levels of ROS and induces cell death.…”