Ox Vitreous Humour. 1. The Residual Protein

Diffusion Regulation in the Vitreous Humor

“…5). Digestion of collagen II from bovine and chick vitreous results in a loss of structural integrity of the vitreous gel (39,40), and we observe the same behavior for ovine vitreous (see Fig. S6).…”

Diffusion Regulation in the Vitreous Humor

“…5). Digestion of collagen II from bovine and chick vitreous results in a loss of structural integrity of the vitreous gel (39,40), and we observe the same behavior for ovine vitreous (see Fig. S6).…”

Vascular Remnants in the Rabbit Vitreous Body. II. Enzyme Digestion and Immunohistochemical Studies

“…and its absence from the surrounding matrix in our plastic-embedded specimens, invites a comment. It was observed in the literature (Pirie, Schmidt and Waters, 1948;Berman and Voaden, 1970), that binding of hyaluronan to other vitreous matrix components is weak. Therefore, hyaluronan may easily be washed out in the course of extensive washing and dehydration procedures.…”

Preservation of the Structure of Enzymatically-Degraded Bovine Vitreous Using Synthetic Proteoglycan Mimics

“…Bishop et al 44 found that removal of hyaluronan (by digestion with Streptomyces hyaluronan lyase) only caused reduction in gel wet weight, and a small percentage of the total hyaluronan (6%) could maintain the vitreous in a fully distended state. Pirie et al 45 found that total removal of collagen fibrils (e.g., by collagenase digestion) converted the vitreous from a gel into a viscous liquid. These results suggested that collagen plays a major role as the vitreous scaffold and hyaluronan works together with it in maintaining vitreous collagen fibril spacing and normal gel structure.…”

Diffusion Regulation in the Vitreous Humor

“…5). Digestion of collagen II from bovine and chick vitreous results in a loss of structural integrity of the vitreous gel (39,40), and we observe the same behavior for ovine vitreous (see Fig. S6).…”

Vascular Remnants in the Rabbit Vitreous Body. II. Enzyme Digestion and Immunohistochemical Studies

“…and its absence from the surrounding matrix in our plastic-embedded specimens, invites a comment. It was observed in the literature (Pirie, Schmidt and Waters, 1948;Berman and Voaden, 1970), that binding of hyaluronan to other vitreous matrix components is weak. Therefore, hyaluronan may easily be washed out in the course of extensive washing and dehydration procedures.…”

Preservation of the Structure of Enzymatically-Degraded Bovine Vitreous Using Synthetic Proteoglycan Mimics

“…Bishop et al 44 found that removal of hyaluronan (by digestion with Streptomyces hyaluronan lyase) only caused reduction in gel wet weight, and a small percentage of the total hyaluronan (6%) could maintain the vitreous in a fully distended state. Pirie et al 45 found that total removal of collagen fibrils (e.g., by collagenase digestion) converted the vitreous from a gel into a viscous liquid. These results suggested that collagen plays a major role as the vitreous scaffold and hyaluronan works together with it in maintaining vitreous collagen fibril spacing and normal gel structure.…”

Diffusion Regulation in the Vitreous Humor

“…5). Digestion of collagen II from bovine and chick vitreous results in a loss of structural integrity of the vitreous gel (39,40), and we observe the same behavior for ovine vitreous (see Fig. S6).…”

Vascular Remnants in the Rabbit Vitreous Body. II. Enzyme Digestion and Immunohistochemical Studies

“…and its absence from the surrounding matrix in our plastic-embedded specimens, invites a comment. It was observed in the literature (Pirie, Schmidt and Waters, 1948;Berman and Voaden, 1970), that binding of hyaluronan to other vitreous matrix components is weak. Therefore, hyaluronan may easily be washed out in the course of extensive washing and dehydration procedures.…”

Preservation of the Structure of Enzymatically-Degraded Bovine Vitreous Using Synthetic Proteoglycan Mimics

“…Bishop et al 44 found that removal of hyaluronan (by digestion with Streptomyces hyaluronan lyase) only caused reduction in gel wet weight, and a small percentage of the total hyaluronan (6%) could maintain the vitreous in a fully distended state. Pirie et al 45 found that total removal of collagen fibrils (e.g., by collagenase digestion) converted the vitreous from a gel into a viscous liquid. These results suggested that collagen plays a major role as the vitreous scaffold and hyaluronan works together with it in maintaining vitreous collagen fibril spacing and normal gel structure.…”