Ovine C-type lectin receptor hFc-fusion protein library – A novel platform to screen for host-pathogen interactions

Vector and Host C-Type Lectin Receptor (CLR)–Fc Fusion Proteins as a Cross-Species Comparative Approach to Screen for CLR–Rift Valley Fever Virus Interactions

“…To evaluate RVFV–CLR interactions in a cross-species approach, mosquito vector CLR–hFc fusion proteins were expressed in addition to available murine and ovine CLR–hFc fusion protein libraries [ 33 , 34 , 35 ]. Initially, a maximum likelihood phylogenetic analysis of the CRDs was performed to compare the CLRs across the different species.…”

“…To identify potential CLR–RVFV interactions in a One Health approach, we employed murine and ovine CLR–human Fc (hFc) fusion protein libraries [ 33 , 34 , 35 ] and extended them with CLR–hFc fusion proteins from the RVFV insect vector Aedes aegypti . Using an ELISA-based binding assay, we screened RVFV binding to CLRs from hosts, as well as insect vectors.…”

C-Type Lectins in Veterinary Species: Recent Advancements and Applications

“…In conclusion, advancements in the understanding of CTL functions in veterinary species will open up new applications in veterinary medicine; yet, the current lack of knowledge clearly highlights the need for further research. To bridge this knowledge gap between model and target species, novel tools, such as recombinant bovine [ 61 ] and ovine [ 60 ] CTL receptor libraries, were recently generated. The role of the identified CTL interactions of veterinary relevant species with pathogens will be unravelled in further studies.…”

“…As such, isolated helminth immunomodulatory compounds mimicking an infestation might be used to suppress autoimmunity in human patients [ 124 ]. Consistently, a desensitizing DC targeting construct composed of the mite allergoid and mannan, a ligand of the murine [ 125 ], ovine [ 60 ], and human [ 126 ] CTL Dectin-2, was described as a potential allergy treatment in dogs [ 127 ]. Another potential therapeutic CTL target is Mincle.…”

“… Recombinant CTL libraries for in vitro screenings allow for the identification of CTL ligands. The murine [ 31 ] and ovine [ 60 ] CTL libraries were expressed as CTL-Fc fusion proteins. For the bovine [ 61 ] library, cow CTL and bacterial biotinylation site coding DNA fragments were fused and expressed in E. coli , yielding biotinylated fusion proteins that can be used for glycan array- and ELISA-based binding studies and high throughput pull-down assays.…”