Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

Yin, Xiao-Yang; Adams, Erwin; Schepdael, Ann Van

doi:10.1002/jssc.202300213

Cited by 7 publications

(4 citation statements)

References 205 publications

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“…Various aspects of this topic, such as CE analysis of native and derivatized AAs with different types of detection (UV‐absorption, fluorescence, contactless conductivity, MS) and the determination of AAs in different matrices including complete peptide and protein hydrolysates, are reported there. Another review article deals with the determination of branched‐chain AAs by LC and CE methods [265].…”

Section: Discussionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Discussionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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“…The derivatization process can be eliminated by changing the stationary phase type or by adding additional ion-pairing reagents to the mobile phase [3,23]. However, ion-pairing reagents are known to inhibit ionization (during MS detection) and shift retention times, which may result in long equilibrium times and irreversible damage to columns and instrumentation [26]. Hydrophilic interaction liquid chromatography (HILIC) has become one of the easiest methods for the measurement of AAs because it eliminates the need for cumbersome derivatization steps as well as the need for ion-pairing reagents to separate AAs, minimizing the possibility of damage to the columns and instrument.…”

Section: Introductionmentioning

confidence: 99%

Quantification of amino acids secreted by yeast cells by hydrophilic interaction liquid chromatography‐tandem mass spectrometry

Yin,

Sousa,

André

et al. 2024

J of Separation Science

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Monitoring the levels of amino acids (AAs) in biological cell cultures provides key information to understand the regulation of cell growth and metabolism. Saccharomyces cerevisiae can naturally excrete AAs, making accurate detection and determination of amino acid levels within the cultivation medium pivotal for gaining insights into this still poorly known process. Given that most AAs lack ultraviolet (UV) chromophores or fluorophores necessary for UV and fluorescence detection, derivatization is commonly utilized to enhance amino acid detectability via UV absorption. Unfortunately, this can lead to drawbacks such as derivative instability, labor intensiveness, and poor reproducibility. Hence, this study aimed to develop an accurate and stable hydrophilic interaction liquid chromatography‐tandem mass spectrometry analytical method for the separation of all 20 AAs within a short 17‐min run time. The method provides satisfactory linearity and sensitivity for all analytes. The method has been validated for intra‐ and inter‐day precision, accuracy, recovery, matrix effect, and stability. It has been successfully applied to quantify 20 AAs in samples of yeast cultivation medium. This endeavor seeks to enhance our comprehension of amino acid profiles in the context of cell growth and metabolism within yeast cultivation media.

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“…Most of the time, eAAA is performed after pre-column derivatization, followed by their separation on chiral stationary phases either by GC [5][6][7][8] or LC [9][10][11][12][13][14][15][16][17][18][19][20]. Derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) is one of the preferred choices for LC-based eAAA, because it is suggested as one of the pharmacopeial methods for amino acid analysis and is widely accepted.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive reversed‐phase×chiral two‐dimensional liquid chromatography coupled to quadrupole‐time‐of‐flight tandem mass spectrometry with post‐first dimension flow splitting for untargeted enantioselective amino acid analysis

Karongo

Horak

Lämmerhofer

2023

J of Separation Science

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This work describes a comprehensive achiral × chiral two‐dimensional liquid chromatography separation for enantioselective amino acid analysis coupled to electrospray ionization‐tandem mass spectrometry detection using data‐independent acquisition. Flow splitting after the first and second dimension separation was utilized for volumetric flow reduction and for enabling a multi‐detector approach (with ultraviolet, fluorescence, charged aerosol, and MS detection), respectively. Derivatization with 6‐aminoquinolyl‐N‐hydroxysuccinimidyl carbamate provided a chromophore, a fluorophore, and an efficient mass tag for efficient ionization in positive electrospray ionization‐mass spectrometry. Chiral columns often have limitations in terms of their chemoselectivity, which may be a problem when complex sample mixtures with structurally related compounds need to be separated. It can be alleviated by a reversed‐phase×chiral two‐dimensional‐liquid chromatography setup, in which the first dimension provides the chemoselectivity and a chiral tandem column constituted of quinine‐carbamate derived weak anion‐exchanger and zwitterionic ion‐exchanger in the second dimension separation of D‐ and L‐amino acid enantiomers. The method was used to control the stereointegrity of the therapeutic peptide octreotide. After hydrolysis, all amino acid constituents were detected with the correct configuration and composition. Some options for flow splitting and integration of destructive detectors in the first dimension separation are outlined.

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Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

Cited by 7 publications

References 205 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Quantification of amino acids secreted by yeast cells by hydrophilic interaction liquid chromatography‐tandem mass spectrometry

Comprehensive reversed‐phase×chiral two‐dimensional liquid chromatography coupled to quadrupole‐time‐of‐flight tandem mass spectrometry with post‐first dimension flow splitting for untargeted enantioselective amino acid analysis

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