Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Isolated in Brazil

Maluta, Renato Pariz; Logue, Catherine M.; Tiba‐Casas, Monique Ribeiro; Meng, Ting; Guastalli, Elisabete Aparecida Lopes; Rojas, Thaís Cabrera Galvão; Montelli, Augusto Cézar; Sadatsune, Teruê; Ramos, Marcelo de Carvalho; Nolan, Lisa K.; Silveira, Wanderley Dias da

doi:10.1371/journal.pone.0105016

Cited by 151 publications

(145 citation statements)

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“…The majority of the sequence types (ST10, ST57 ST 224, ST354 and S624) identified in the current study from FQ and ESC resistant isolates have been previously described and are globally distributed amongst humans as well as animals (Fernandes et al, 2016;Kim et al, 2011;Maluta et al, 2014;Sola-Gines et al, 2015;Zhao et al, 2014). ST2705 and ST6053 are host restricted and have been previously isolated from poultry and wild birds (Dahms et al, 2015;Day et al, 2016;Jones-Dias et al, 2015).…”

Section: Discussionmentioning

confidence: 60%

“…The current study reported a variation in the ST among the clinical FQ resistant isolates (keeping in mind that there was only two clinical FQ resistant isolates) with one assigned as ST224 while the other was ST10. Both STs have previously been identified in horses, dogs, buffalos and human pathogenic and non-pathogenic E. coli Aizawa et al, 2014;Dissanayake et al, 2014;Maluta et al, 2014;Manges and Johnson, 2012;Pires-dos-Santos et al, 2013;Vignaroli et al, 2012;Zhao et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Besides the classical MLST scheme, several other MLST classifications have been established targeting fewer genes (Sahl et al, 2012;Tchesnokova et al, 2013;Weissman et al, 2012). The most common STs detected among APEC and AFEC are globally disseminated and have emerged among different species of animals, regardless of pathogenicity, such as ST354 and ST10 Dissanayake et al, 2014;Kim et al, 2011;Maluta et al, 2014;Manges and Johnson, 2012;Mora et al, 2011;Pires-dos-Santos et al, 2013;Schaufler et al, 2015).…”

Section: Multilocus Sequence Typing (Mlst)mentioning

confidence: 99%

“…Historically, it was assumed that fimbrial adhesins were encoded by the crl gene. However, recently the csgA gene was recognised as encoding for curli, which have been found to be expressed equally among APEC and AFEC (Amabile de Campos et al, 2005;Cunha et al, 2017;Maluta et al, 2014). This may indicate that curli fimbriae initiate colonisation for both APEC and AFEC.…”

Section: Curlimentioning

confidence: 99%

“…Despite all of the improvements and modernisation in poultry production systems and the fact that avian pathogenic Escherichia coli (APEC) has been a recognised cause of avian colibacillosis for over a century (Collingwood et al, 2014;Dho-Moulin and Fairbrother, 1999;Johnson and Russo, 2002;Nolan et al, 2013), it remains as one of the major pathogens posing a considerable challenge to the global poultry industry (Collingwood et al, 2014;Dho-Moulin and Fairbrother, 1999;Johnson and Russo, 2002;Nolan et al, 2013 Avian pathogenic E. coli can act as a primary or secondary pathogen (Antão et al, 2008;Bauchart et al, 2010;Kemmett et al, 2013;Kohler and Dobrindt, 2011;Maluta et al, 2014) and can result in localised and/or systemic infections in birds (Guabiraba and Schouler, 2015;Nolan et al, 2013;Rodriguez-Siek et al, 2005a). The severity of the disease depends on the virulence of APEC strains, chicken age and immune status and the presence of predisposing risk factors (Dho-Moulin and Fairbrother, 1999;Guabiraba and Schouler, 2015;Vandekerchove et al, 2004a).…”

Section: Introductionmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Multilocus Sequence Typing (Mlst)mentioning

confidence: 99%

Section: Curlimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Colibacillosis

Nolan¹,

Vaillancourt²,

Barbieri³

et al. 2019

Diseases of Poultry

116

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Modeling the effect of simultaneous use of allyl isothiocyanate and cinnamaldehyde on high hydrostatic pressure inactivation of Uropathogenic and Shiga toxin‐producingEscherichia coli in ground chicken

et al. 2020

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BACKGROUNDA combination of high‐pressure processing (HPP) and antimicrobials is a well‐known approach for enhancing the microbiological safety of foods. However, few studies have applied multiple antimicrobials simultaneously with HPP, which could be an additional hurdle for microbial inactivation. The present study applied a full factorial design to investigate the impact of HPP (225–325 MPa; 10–20 min), allyl isothiocyanate (AITC) (0.3–0.9 g kg–1) and trans‐cinnamaldehyde (tCinn) (1.0–2.0 g kg–1) on the inactivation of Shiga toxin‐producing Escherichia coli (STEC) O157:H7 and uropathogenic E. coli (UPEC) in ground chicken meat.RESULTSThe regulatory requirement of 5‐log reduction was achieved at 305 MPa, 18 min, 0.8 g kg–1 AITC and 1.7 g kg–1 tCinn for STEC O157:H7 and at 293 MPa, 16 min, 0.6 g kg–1 AITC and 1.6 g kg–1 tCinn for UPEC, as specified by response surface analysis and verified via experiments. The surviving population was eliminated by post‐treatment storage of 9 days at 10 °C. The developed linear regression models showed r2 > 0.9 for the E. coli inactivation. The developed dimensionless non‐linear regression models covered a factorial range slightly wider than the original experimental limit, with probability Pr > F (< 0.0001).CONCLUSIONSimultaneous use of AITC and tCinn reduced not only the necessary concentration of each compound, but also the intensity of high‐pressure treatments, at the same time achieving a similar level of microbial inactivation. STEC O157:H7 was found to be more resistant than UPEC to the HPP‐AITC‐tCinn stress. The developed models may be applied in commercial application to enhance the microbiological safety of ground chicken meat. Published 2020. This article is a U.S. Government work and is in the public domain in the USA.

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Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Isolated in Brazil

Cited by 151 publications

References 38 publications

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Colibacillosis

Modeling the effect of simultaneous use of allyl isothiocyanate and cinnamaldehyde on high hydrostatic pressure inactivation of Uropathogenic and Shiga toxin‐producingEscherichia coli in ground chicken

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