Overexpression VaPYL9 improves cold tolerance in tomato by regulating key genes in hormone signaling and antioxidant enzyme

Abstract

Background Abscisic acid (ABA) has been reported in controlling plant growth and development, and particularly dominates a role in resistance to abiotic stress. The Pyrabactin Resistance1/PYR1-Like /Regulatory Components of ABA receptors (PYR1/PYL/RCAR) gene family, of which the PYL9 is a positive regulator related to stress response in ABA signaling transduction. Although the family has been identified in grape, detailed VaPYL9 function in cold stress remains unknown. Results In order to explore the cold tolerance mechanism in grape, VaPYL9 was cloned from Vitis amurensis. The subcellular localization showed that VaPYL9 was mainly expressed in the plasma membrane. Yeast two-hybrid (Y2H) showed VaPCMT might be a potential interaction protein of VaPYL9. Through the overexpression of VaPYL9 in tomatoes, results indicated transgenic plants had higher antioxidant enzyme activities and proline content, lower malondialdehyde (MDA) and H2O2 content, and improving the ability to scavenge reactive oxygen species than wild-type (WT). Additionally, ABA content and the ratio of ABA/IAA kept a higher level than WT. Quantitative real-time PCR (qRT-PCR) showed that VaPYL9, SlNCED3, SlABI5, and antioxidant enzyme genes (POD, SOD, CAT) were up-regulated in transgenic tomatoes. Transcriptome sequencing (RNA-seq) found that VaPYL9 overexpression caused the upregulation of key genes PYR/PYL, PYL4, MAPK17/18, and WRKY in transgenic tomatoes under cold stress. Conclusion Overexpression VaPYL9 enhances cold resistance of transgenic tomatoes mediated by improving antioxidant enzymes activity, reducing membrane damages, and regulating key genes in plant hormones signaling and antioxidant enzymes.

Integrative physiological, transcriptome, and metabolome analyses reveal the associated genes and metabolites involved in cold stress response in common vetch (Vicia sativa L.)

“…Heatmap of transcript levels for genes and contents for metabolites that are involved in the biosynthesis of phenylpropanoids. signaling pathway, and previous studies reported that PYL genes are positive regulators related to stress response in ABA signaling transduction, such as PYL4 and PYL9 (Nai et al, 2022;Ren et al, 2022). In our study, we found that three PYR/PYL genes (c25218.graph_c0, c34730.graph_c0, and c49758.graph_c0) were significantly induced by cold treatment (Figure S18a), which suggested that the ABA signaling pathway plays an important role in the response of V. sativa to cold stress.…”

“…A total of 16 DEGs related to ABA signal transduction were obtained in the present study, and the expression level of most genes was induced during cold treatment except for three genes (Figure S17a). The pyrabactin resistance (PYR)/PYR‐like (PYL)/regulatory component of ABA receptor (RCAR) proteins that have been characterized as ABA receptors function as the core components in the ABA signaling pathway, and previous studies reported that PYL genes are positive regulators related to stress response in ABA signaling transduction, such as PYL4 and PYL9 (Nai et al, 2022; Ren et al, 2022). In our study, we found that three PYR / PYL genes (c25218.graph_c0, c34730.graph_c0, and c49758.graph_c0) were significantly induced by cold treatment (Figure S18a), which suggested that the ABA signaling pathway plays an important role in the response of V. sativa to cold stress.…”

Decoding VaCOLD1 Function in Grapevines: A Membrane Protein Enhancing Cold Stress Tolerance

“…Furthermore, ABA reduced the inhibitory effect of cold stress on the rate of CO 2 fixation in grape plants by stimulating the antioxidant enzyme system and alleviating cold-induced stomatal closure . Many genes are activated in response to cold stress that are likewise activated in response to ABA and osmotic stress . Overexpression of the AtABF3 gene enhances tolerance to drought and cold stress in transgenic lettuce ( Lactuca sativa ) .…”

“…45 Many genes are activated in response to cold stress that are likewise activated in response to ABA and osmotic stress. 56 Overexpression of the AtABF3 gene enhances tolerance to drought and cold stress in transgenic lettuce (Lactuca sativa). 57 Another gene ABF4/AREB2, an ABA-responsive element-binding factor and a member of the bZIP transcription factors, was likewise increased by cold.…”

Evolution of the 14-3-3 gene family in monocotyledonous and dicotyledonous and validation of MdGRF13 function in transgenic Arabidopsis thaliana

“…The constructed recombinant plasmids were co-transformed into yeast cells of strain Y2H and cultured on SD/-Leu/-Trp medium at 30°C for 3 days. Positive colonies were picked and transferred to SD/-Leu/-Trp/-Ade/-His + X-α-Gal medium to examine potential physical binding and interaction (Nai et al 2022).…”