Overexpression of two ATNAC3-related genes improves drought and salt tolerance in tomato (Solanum lycopersicum L.)

Al‐Abdallat, Ayed; Ali-Sheikh-Omar, M. A.; Alnemer, Loai M.

doi:10.1007/s11240-014-0652-8

Cited by 24 publications

(11 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, Arabidopsis plants ectopically expressing GmNAC109 displayed remarkably improved tolerance to drought, with higher survival rates after a 12-day-drought imposition in comparison with the WT (Figure 2a–c). Enhancement of drought tolerance in transgenic plants by overexpressing/ectopically expressing NAC genes has been well documented in many previous studies, such as those that used tomato SlNAC3 [58], rice SNAC1 [59], SNAC3 [8] and ONAC022 [60], pumpkin ( Cucurbita moschata ) CmNAC1 [61], and soybean GmNAC085 [36,37] and GmNACS49 [38]. Such transgenic studies have also revealed diverse mechanisms of the NAC TFs in contributing to the enhanced drought tolerance of the transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

Ectopic Expression of Glycine max GmNAC109 Enhances Drought Tolerance and ABA Sensitivity in Arabidopsis

et al. 2019

View full text Add to dashboard Cite

The NAC (NAM, ATAF1/2, CUC2) transcription factors are widely known for their various functions in plant development and stress tolerance. Previous studies have demonstrated that genetic engineering can be applied to enhance drought tolerance via overexpression/ectopic expression of NAC genes. In the present study, the dehydration- and drought-inducible GmNAC109 from Glycine max was ectopically expressed in Arabidopsis (GmNAC109-EX) plants to study its biological functions in mediating plant adaptation to water deficit conditions. Results revealed an improved drought tolerance in the transgenic plants, which displayed greater recovery rates by 20% to 54% than did the wild-type plants. In support of this finding, GmNAC109-EX plants exhibited lower water loss rates and decreased endogenous hydrogen peroxide production in leaf tissues under drought, as well as higher sensitivity to exogenous abscisic acid (ABA) treatment at germination and early seedling development stages. In addition, analyses of antioxidant enzymes indicated that GmNAC109-EX plants possessed stronger activities of superoxide dismutase and catalase under drought stress. These results together demonstrated that GmNAC109 acts as a positive transcriptional regulator in the ABA-signaling pathway, enabling plants to cope with adverse water deficit conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Ectopic Expression of Glycine max GmNAC109 Enhances Drought Tolerance and ABA Sensitivity in Arabidopsis

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Ooka [ 8 ] classified NAC proteins into Groups I and II, with Group I divided into 14 subgroups. The NAC transcription factors in subgroups ATAF [ 23 ], AtNAC3 [ 24 ], and OsNAC3 [ 25 ] are involved in plant stress responses. The conserved sequences and phylogenetic relationships have been analyzed.…”

Section: Discussionmentioning

confidence: 99%

The NAC-type transcription factor CaNAC46 regulates the salt and drought tolerance of transgenic Arabidopsis thaliana

Wang

Dai

et al. 2021

BMC Plant Biol

View full text Add to dashboard Cite

Background The NAC (NAM, ATAF1/ATAF2, and CUC2) transcription factors belong to a large family of plant-specific transcription factors in monocot and dicot species. These transcription factors regulate the expression of stress tolerance-related genes that protect plants from various abiotic stresses, including drought, salinity, and low temperatures. Results In this study, we identified the CaNAC46 transcription factor gene in Capsicum annuum. Its open reading frame was revealed to comprise 921 bp, encoding a protein consisting of 306 amino acids, with an isoelectric point of 6.96. A phylogenetic analysis indicated that CaNAC46 belongs to the ATAF subfamily. The expression of CaNAC46 was induced by heat, cold, high salt, drought, abscisic acid, salicylic acid, and methyl jasmonate treatments. Thus, CaNAC46 may be important for the resistance of dry pepper to abiotic stresses. A subcellular localization analysis confirmed that CaNAC46 is localized in the nucleus. The overexpression of CaNAC46 improved the tolerance of transgenic Arabidopsis thaliana plants to drought and salt stresses. The CaNAC46-overexpressing lines had longer roots and more lateral roots than wild-type lines under prolonged drought and high salt stress conditions. Additionally, CaNAC46 affected the accumulation of reactive oxygen species (ROS). Moreover, CaNAC46 promoted the expression of SOD, POD, RD29B, RD20, LDB18, ABI, IAA4, and P5CS. The malondialdehyde contents were higher in TRV2-CaNAC46 lines than in wild-type plants in response to drought and salt stresses. Furthermore, the expression levels of stress-responsive genes, such as ABA2, P5CS, DREB, RD22, CAT, and POD, were down-regulated in TRV2-CaNAC46 plants. Conclusions Under saline and drought conditions, CaNAC46 is a positive regulator that activates ROS-scavenging enzymes and enhances root formation. The results of our study indicate CaNAC46 is a transcriptional regulator responsible for salinity and drought tolerance and suggest the abiotic stress-related gene regulatory mechanisms controlling this NAC transcription factor are conserved between A. thaliana and pepper.

show abstract

“…This finding was later confirmed through qRT-PCR analyses using several selected differentially expressed miRNAs. miRNAs may regulate host defenses against pathogens, including viruses, by suppressing pathogen multiplication at the post-transcriptional level [13,52]. Several stress-responsive miRNAs (e.g., miR168, miR169, and miR482) have been reported to target transcription factors controlling host resistance to virus infection [53][54][55].…”

Section: Discussionmentioning

confidence: 99%

Identification of microRNAs regulated by tobacco curly shoot virus co-infection with its betasatellite in Nicotiana benthamiana

Zhou

et al. 2019

Virol J

View full text Add to dashboard Cite

Background: MicroRNAs (miRNAs) are a class of 21-24 nucleotide endogenous non-coding small RNAs that play important roles in plant development and defense responses to biotic and abiotic stresses. Tobacco curly shoot virus (TbCSV) is a monopartite begomovirus, cause leaf curling and plant stunting symptoms in many Solanaceae plants. The betasatellite of TbCSV (TbCSB) induces more severe symptoms and enhances virus accumulation when co-infect the plants with TbCSV. Methods: In this study, miRNAs regulated by TbCSV and TbCSB co-infection in Nicotiana benthamiana were characterized using high-throughput sequencing technology.Results: Small RNA sequencing analysis revealed that a total of 13 known miRNAs and 42 novel miRNAs were differentially expressed in TbCSV and TbCSB co-infected N. benthamiana plants. Several potential miRNA-targeted genes were identified through data mining and were involved in both catalytic and metabolic processes, in addition to plant defense mechanisms against virus infections according to Gene Ontology (GO) analyses. In addition, the expressions of several differentially expressed miRNAs and their miRNA-targeted gene were validated through quantitative real time polymerase chain reaction (qRT-PCR) approach.Conclusions: A large number of miRNAs are identified, and their target genes, functional annotations also have been explored. Our results provide the information on N. benthamiana miRNAs and would be useful to further understand miRNA regulatory mechanisms after TbCSV and TbCSB co-infection.

show abstract

Overexpression of two ATNAC3-related genes improves drought and salt tolerance in tomato (Solanum lycopersicum L.)

Cited by 24 publications

References 52 publications

Ectopic Expression of Glycine max GmNAC109 Enhances Drought Tolerance and ABA Sensitivity in Arabidopsis

Ectopic Expression of Glycine max GmNAC109 Enhances Drought Tolerance and ABA Sensitivity in Arabidopsis

The NAC-type transcription factor CaNAC46 regulates the salt and drought tolerance of transgenic Arabidopsis thaliana

Identification of microRNAs regulated by tobacco curly shoot virus co-infection with its betasatellite in Nicotiana benthamiana

Contact Info

Product

Resources

About