2016
DOI: 10.1007/s10616-016-9944-7 View full text |Buy / Rent full text
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Abstract: Hepatocytes differentiated from induced pluripotent stem cells and adult stem cells could be utilized as a tool for the study of liver diseases, screening for drug metabolism and hepatotoxicity. Thus further investigation of the method to efficiently generate hepatocytes is in great need. Bone Mesenchymal Stem Cells (BMSCs) were collected from rat femurs and tibias. FOXA2 and HNF1α genes were constructed into a lentiviral vector and introduced into BMSCs by a lentivirus-mediated overexpression system. Three we… Show more

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“…RNA interference has been developed as an important means to study gene function. Lentiviral vectors have little immune response, and are able to infect non-dividing cells with a high transfection efficiency, therefore, they are also often used to study the function of genes [ 14 , 15 , 23 26 ]. In this study, we used lentiviral vector for over-expression of C9orf116 and siRNA to knockdown the expression of C9orf116, and further analyzed its possible role in BRL-3A cells.…”
Section: Discussionmentioning
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rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…RNA interference has been developed as an important means to study gene function. Lentiviral vectors have little immune response, and are able to infect non-dividing cells with a high transfection efficiency, therefore, they are also often used to study the function of genes [ 14 , 15 , 23 26 ]. In this study, we used lentiviral vector for over-expression of C9orf116 and siRNA to knockdown the expression of C9orf116, and further analyzed its possible role in BRL-3A cells.…”
Section: Discussionmentioning
“…Vector particles were produced in HEK293T cells by transient cotransfection involving a three-plasmid expression system. Viral packaging was processed according to Dai et al [ 14 ] and Ding et al [ 15 ]. The concentrated virus particles were suspended in PBS and stored at -80°C.…”
Section: Methodsmentioning
“…The method to package the lentivirus was described previously . Recombinant plasmid pCDH‐CMV‐MCS‐EF1‐copGFP‐SPINK1 was synthesized by Generay (Shanghai, China).…”
Section: Methodsmentioning
“…The method of Ding et al (Ding et al, 2016) was used to package the lentivirus. Recombinant plasmid pCDH-CMV-MCS-EF1-copGFP-GADD45α was synthesized by Generay (Shanghai, China).…”
Section: Methodsmentioning