Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells

Zhang, Yingzi; Tian, Jiao; Qu, Chi; Peng, Yang; Lei, Jinwei; Li, Kang; Zong, Beige; Sun, Lu; Liu, Shengchun

doi:10.1007/s12282-021-01221-4

Cited by 28 publications

(25 citation statements)

References 28 publications

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“…Meanwhile, MCC method of the CytoHubba plug-in was used to calculate the top 5 proteins as hubproteins. In the BF-TK/GCV / BF-TK group, the top 5 hubproteins were AMBP, HRG, APCS, HPX and GIG25 (Figure 4D), some proteins (HRG, HPX and GIG25) are positively associated with tumor metastasis (Mondaca et al, 2021; Niu et al, 2021; Zhang et al, 2021a). Besides, PPI network showed that CEBPB, NF-κB1, MTOR, HIF-1A, VCAM1 and CXCL12 were interacted directly or indirectly (Figure 4E).…”

Section: Resultsmentioning

confidence: 99%

“…Hemopexin (HPX) promotes invasion and metastasis of the pancreatic cancer and colorectal carcinoma cells(Niu et al, 2021). GIG25 is a serpin family a member 3 (or SERPINA3) and overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells(Zhang et al, 2021b). PELO was upregulated by BF-TK/GCV treatment and enriched in mRNA surveillance pathway, However, it was listed out of top 30 pathways (data not showed in Figure 1C).…”

Section: Discussionmentioning

confidence: 99%

“…The Cancer Genome Atlas-Stomach Adenocarcinoma (TCGA-STAD) RNA-seq gene expression data and clinical data were obtained from the TCGA Data Portal (https://portal.gdc.cancer.gov/) (Xu et al, 2020). The survival probability of high-and low-expression gene groups and gene expression correlation analysis were analyzed by GEPIA (http://gepia.cancerpku.cn/) (Li et al, 2021b;Xue et al, 2022).…”

Section: Functional Network and Clinical Significance Analysismentioning

confidence: 99%

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Bifidobacterium infantis-mediated herpes simplex virus-TK/ganciclovir treatment inhibits cancer metastasis

Wang

Shen

et al. 2022

Preprint

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Previous studies have found that Bifidobacterium infantis-mediated herpes simplex virus-TK/ganciclovir (BF-TK/GCV) reduces the expression of VEGF and CD146 which implies tumor metastasis inhibition. However, the mechanism of BF-TK/GCV inhibits tumor metastasis is still not fully studied. Here, we comprehensively identified and quantified protein expression profiling for the first time in gastric cancer (GC) cells MKN-45 upon BF-TK/GCV treatment using quantitative proteomics. A total of 159 and 72 differential expression proteins (DEPs) were significantly changed in BF-TK/GCV / BF-TK and BF-TK/GCV / BF/GCV groups. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis showed some enriched metastasis-related pathways such as gap junction and cell adhesion molecules pathways. Moreover, transwell assay proved that BF-TK/GCV inhibited the invasion and migration of tumor cells. Furthermore, immunohistochemistry (IHC) demonstrated that BF-TK/GCV reduced the expression of HIF-1A, MTOR, NF-?B1-p105, VCAM1, CEBPB and CXCL12, which were associated with tumor metastasis. In summary, besides apoptosis, BF-TK/GCV also inhibited tumor metastasis, which deepened and expanded the understanding of BF-TK/GCV anti-tumor mechanisms.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Functional Network and Clinical Significance Analysismentioning

confidence: 99%

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Bifidobacterium infantis-mediated herpes simplex virus-TK/ganciclovir treatment inhibits cancer metastasis

Wang

Shen

et al. 2022

Preprint

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“…Although its exact biological function is not known, it has been described in association with diverse pathologies, such as acute kidney injury, cardiovascular disease, and (lung) cancer [ 37 , 38 , 39 , 40 ]. It was described to promote metastasis and the epithelial–mesenchymal transition in breast cancer [ 41 ] and was found to be upregulated in lung cancer in comparison to control patients [ 42 ]. In our study, the highest SERPINA3 abundance was found in COPD patients ( Supplementary Figure S3 ), again demonstrating the need for the direct comparison of clinically relevant patient groups.…”

Section: Discussionmentioning

confidence: 99%

Plasma Proteomics Enable Differentiation of Lung Adenocarcinoma from Chronic Obstructive Pulmonary Disease (COPD)

Bracht

Kleefisch

Schork

et al. 2022

IJMS

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Chronic obstructive pulmonary disease (COPD) is a major risk factor for the development of lung adenocarcinoma (AC). AC often develops on underlying COPD; thus, the differentiation of both entities by biomarker is challenging. Although survival of AC patients strongly depends on early diagnosis, a biomarker panel for AC detection and differentiation from COPD is still missing. Plasma samples from 176 patients with AC with or without underlying COPD, COPD patients, and hospital controls were analyzed using mass-spectrometry-based proteomics. We performed univariate statistics and additionally evaluated machine learning algorithms regarding the differentiation of AC vs. COPD and AC with COPD vs. COPD. Univariate statistics revealed significantly regulated proteins that were significantly regulated between the patient groups. Furthermore, random forest classification yielded the best performance for differentiation of AC vs. COPD (area under the curve (AUC) 0.935) and AC with COPD vs. COPD (AUC 0.916). The most influential proteins were identified by permutation feature importance and compared to those identified by univariate testing. We demonstrate the great potential of machine learning for differentiation of highly similar disease entities and present a panel of biomarker candidates that should be considered for the development of a future biomarker panel.

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“…In prostate cancer, SERPINA3 promotes aerobic glycolysis and autophagy while inhibiting apoptosis of cancer cells ( Xing et al, 2021 ). In glioblastoma and breast cancer, high expression of SERPINA3 is closely related to cancer cell proliferation, invasion, migration, and epithelial-mesenchymal transition, and thus leads to poor prognosis and tumor recurrence ( Nimbalkar et al, 2021 ; Zhang et al, 2021 ). Similar phenomena can also be observed in endometrial cancer, liver cancer, colon cancer and so on ( Cao et al, 2018 ; Ko et al, 2019 ; Zhou et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

A panel of seven immune-related genes can serve as a good predictive biomarker for cervical squamous cell carcinoma

Dai

Pan²,

Chen³

et al. 2022

Front. Genet.

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Objective: Cervical cancer is one of the most common gynecological malignancies. The interaction between tumor microenvironment and immune infiltration is closely related to the progression of cervical squamous cell carcinoma (CSCC) and patients’ prognosis. Herein, a panel of immune-related genes was established for more accurate prognostic prediction.Methods: The transcriptome information of tumor and normal samples were obtained from TCGA-CSCC and GTEx. Differentially expressed genes (DEGs) were defined from it. Immune-related genes (IRGs) were retrieved from the ImmPort database. After removing the transcriptome data which not mentioned in GSE44001, IR-DEGs were preliminarily identified. Then, TCGA-CSCC samples were divided into training and testing set (3:1) randomly. Univariate Cox analysis, LASSO regression analysis and multivariate Cox analysis were used in turn to construct the signature to predict the overall survival (OS) and disease-free survival (DFS). External validation was performed in GSE44001, and initial clinical validation was performed by qRT-PCR. Function enrichment analysis, immune infiltration analysis and establishment of nomogram were conducted as well.Results: A prognostic prediction signature consisting of seven IR-DEGs was established. High expression of NRP1, IGF2R, SERPINA3, TNF and low expression of ICOS, DES, HCK suggested that CSCC patients had shorter OS (POS<0.001) and DFS (PDFS<0.001). AUC values of 1-, 3-, five- year OS were 0.800, 0.831 and 0.809. Analyses in other validation sets showed good consistency with the results in training set. The signature can serve as an independent prognostic factor for OS (HR = 1.166, p < 0.001). AUC values of 1-, 3-, five- year OS based on the nomogram were 0.769, 0.820 and 0.807. Functional enrichment analysis suggested that these IR-DEGs were associated with receptor interaction and immune cell activity. Immune infiltration analysis indicated that patients in high-risk group had lower immune infiltration, weaker immune function, and were more likely to benefit from immune checkpoint inhibitor therapy. Through qRT-PCR on clinical samples, expression of NRP1, IGF2R, SERPINA3 and TNF were significantly upregulated in tumor tissue, while ICOS and DES were significantly downregulated.Conclusion: To conclude, the immune-related signature can provide strong support for exploration of immune infiltration, prediction of prognosis and response to immunotherapy through stratify CSCC patients into subgroups.

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Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells

Cited by 28 publications

References 28 publications

Bifidobacterium infantis-mediated herpes simplex virus-TK/ganciclovir treatment inhibits cancer metastasis

Bifidobacterium infantis-mediated herpes simplex virus-TK/ganciclovir treatment inhibits cancer metastasis

Plasma Proteomics Enable Differentiation of Lung Adenocarcinoma from Chronic Obstructive Pulmonary Disease (COPD)

A panel of seven immune-related genes can serve as a good predictive biomarker for cervical squamous cell carcinoma

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