Overexpression of polygalacturonase-inhibiting protein (PGIP) gene from Hypericum perforatum alters expression of multiple defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens in tobacco

Hou, Weina; Singh, Rupesh Kumar; Zhao, Pan; Martins, Viviana; Aguilar, Emmanuel; Canto, Tomás; Tenllado, Francisco; Franklin, Gregory; Dias, Alberto Carlos Pires

doi:10.1016/j.jplph.2020.153268

Cited by 10 publications

(5 citation statements)

References 58 publications

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“…3 D-F). PGIPs (polygalacturonase inhibiting proteins) are associated with the plant cell wall and play a crucial role in plant defence [ 35 ], and they are upregulated in BS. LSH10 was a probable transcription regulator that acts as a developmental regulator by promoting cell growth in response to light, and it is upregulated in BS.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear

Wang

Liu

et al. 2021

BMC Plant Biol

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Background Browning spot (BS) disorders seriously affect the appearance quality of ‘Huangguan’ pear and cause economic losses. Many studies on BS have mainly focused on physiological and biochemical aspects, and the molecular mechanism remains unclear. Results In the present study, the structural characteristics of ‘Huangguan’ pear with BS were observed via scanning electron microscopy (SEM), the water loss and brown spots were evaluated, and transcriptomic and metabolomics analyses were conducted to reveal the molecular mechanism underlying ‘Huangguan’ pear skin browning disorder. The results showed that the occurrence of BS was accompanied by a decrease in the wax layer and an increase in lignified cells. Genes related to wax biosynthesis were downregulated in BS, resulting in a decrease in the wax layer in BS. Genes related to lignin were upregulated at the transcriptional level, resulting in upregulation of metabolites related to phenylpropanoid biosynthesis. Expression of calcium-related genes were upregulated in BS. Cold-induced genes may represent the key genes that induce the formation of BS. In addition, the results demonstrated that exogenous NaH2PO4·2H2O and ABA treatment could inhibit the incidence of BS during harvest and storage time by increasing wax-related genes and calcium-related genes expression and increasing plant resistance, whereas the transcriptomics results indicated that GA3 may accelerate the incidence and index of BS. Conclusions The results of this study indicate a molecular mechanism that could explain BS formation and elucidate the effects of different treatments on the incidence and molecular regulation of BS.

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Section: Resultsmentioning

confidence: 99%

Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear

Wang

Liu

et al. 2021

BMC Plant Biol

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“…Consistently, the overexpression of GhPGIP1 enhanced the resistance of cotton (Gossypium hirsutum L.) to Verticillium and Fusarium wilts, and resistance was severely reduced when the gene was silenced [60]. The overexpression of HpPGIP led to the upregulation of key genes involved in hormone signaling, microRNA-based gene silencing, homeostasis of reactive oxygen species, and the phenylpropanoid pathway [20]. There were increased expression levels of disease resistance-related genes, including VvPGIP in fruit inoculated with B. cinerea infection [47].…”

Section: Application Of Pgip In Plant Disease Resistance Genetic Engineeringmentioning

confidence: 83%

“…Similarly, Chen et al [19] showed via qRT-PCR that good resistant wheat variety YSBR1 has higher PGIP expression than a variety with poor resistance. Tobacco genetically integrated with the PGIP gene from Hypericum perforatum exhibited increased anti-disease ability [20]. A study of fungal resistance on two varieties of wheat found PGIP expression level proportional to the anti-disease ability [21].…”

Section: Discovery Of Pgip and Its Variety And Diversitymentioning

confidence: 99%

Recent Advances in Understanding the Function of the PGIP Gene and the Research of Its Proteins for the Disease Resistance of Plants

Cheng

Lin

et al. 2021

Applied Sciences

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Polygalacturonase-inhibiting protein (PGIP) is an important plant biochemical anti-disease factor. PGIP has a leucine-rich repeat structure that can selectively bind and inhibit the activity of endo-polygalacturonase (endo-PG) in fungi, playing a key role in plant disease resistance. The regulation of PGIP in plant disease resistance has been well studied, and the effect of PGIP to increase disease resistance is clear. This review summarizes recent advances in understanding the PGIP protein structure, the PGIP mechanism of plant disease resistance, and anti-disease activity by PGIP gene transfer. This overview should contribute to a better understanding of PGIP function and can help guide resistance breeding of PGIP for anti-disease effects.

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“…It has been reported that both biotic and abiotic stress can induce the expression of PGIP . Therefore, PGIP plays an essential role in plant defense response [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Alfalfa (Medicago sativa L.) Roots in Response to Rhizobium Nodulation and Salt Stress

Wang

Zhang

et al. 2022

Genes

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(1) Background: Alfalfa is an important legume forage throughout the world. Although alfalfa is considered moderately tolerant to salinity, its production and nitrogen-fixing activity are greatly limited by salt stress. (2) Methods: We examined the physiological changes and proteomic profiles of alfalfa with active nodules (NA) and without nodules (NN) under NaCl treatment. (3) Results: Our data suggested that NA roots showed upregulation of the pathways of abiotic and biotic stress responses (e.g., heat shock proteins and pathogenesis-related proteins), antioxidant enzyme synthesis, protein synthesis and degradation, cell wall degradation and modification, acid phosphatases, and porin transport when compared with NN plants under salt stress conditions. NA roots also upregulated the processes or proteins of lipid metabolism, heat shock proteins, protein degradation and folding, and cell cytoskeleton, downregulated the DNA and protein synthesis process, and vacuolar H+-ATPase proteins under salt stress. Besides, NA roots displayed a net H+ influx and low level of K+ efflux under salt stress, which may enhance the salt tolerance of NA plants. (4) Conclusions: The rhizobium symbiosis conferred the host plant salt tolerance by regulating a series of physiological processes to enhance stress response, improve antioxidant ability and energy use efficiency, and maintain ion homeostasis.

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Overexpression of polygalacturonase-inhibiting protein (PGIP) gene from Hypericum perforatum alters expression of multiple defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens in tobacco

Cited by 10 publications

References 58 publications

Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear

Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear

Recent Advances in Understanding the Function of the PGIP Gene and the Research of Its Proteins for the Disease Resistance of Plants

Proteomic Analysis of Alfalfa (Medicago sativa L.) Roots in Response to Rhizobium Nodulation and Salt Stress

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