Overexpression of GmHsp90s, a Heat Shock Protein 90 (Hsp90) Gene Family Cloning from Soybean, Decrease Damage of Abiotic Stresses in Arabidopsis thaliana

Xu, Jing; Xue, Chenchen; Dong, Xue; Zhao, Jinming; Gai, Junyi; Guo, Na

doi:10.1371/journal.pone.0069810

Cited by 96 publications

(63 citation statements)

References 60 publications

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“…HSP90 can bind to Argonaute (AGO1) and facilitate the assembly of plant RNA‐induced silencing complexes (Iki et al , ). Although several groups have suggested the involvement of HSP90 in plant drought stress response in different species (Song et al , ; Xu et al , ), the underlying regulating mechanism and downstream components remain elusive. In this study, we demonstrated that MeHSP90.9 interacts with MeWRKY20 and MeCatalase1 in nuclei and peroxisome in tobacco leaves, respectively (Fig.…”

Section: Discussionmentioning

confidence: 99%

The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava

Wei

et al. 2020

New Phytologist

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Summary The 90 kDa heat shock protein (HSP90) is widely involved in various developmental processes and stress responses in plants. However, the molecular chaperone HSP90‐constructed protein complex and its function in cassava remain elusive. In this study, we report that HSP90 is essential for drought stress resistance in cassava by regulating abscisic acid (ABA) and hydrogen peroxide (H2O2) using two specific protein inhibitors of HSP90 (geldanamycin (GDA) and radicicol (RAD)). Among 10 MeHSP90s, the transcript of MeHSP90.9 is largely induced during drought stress. Further investigation identifies MeWRKY20 and MeCatalase1 as MeHSP90.9‐interacting proteins. MeHSP90.9‐, MeWRKY20‐, or MeCatalase1‐silenced plants through virus‐induced gene silencing display drought sensitivity in cassava, indicating that they are important to drought stress response. MeHSP90.9 can promote the direct transcriptional activation of MeWRKY20 on the W‐box element of MeNCED5 promoter, encoding a key enzyme in ABA biosynthesis. Moreover, MeHSP90.9 positively regulates the activity of MeCatalase1, and MeHSP90.9‐silenced cassava leaves accumulate more H2O2 under drought stress. Taken together, we demonstrate that the MeHSP90.9 chaperone complex is a regulator of drought stress resistance in cassava.

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Section: Discussionmentioning

confidence: 99%

The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava

Wei

et al. 2020

New Phytologist

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“…The other HSP20 families were up regulated in both cultivars, but were not passed cut-off in Daewon. Some of HSP90 genes include GmHsp90A1 (Glyma.09g131500) and GMHsp90C1.2 (Glyma.02g124500) were reported as rapidly accumulate and be maintained for a long period (Xu et al 2013). These genes were found in up-regulation genes of Hwangkeum, but did not found to be DEGs in Daewon.…”

Section: Discussionmentioning

confidence: 99%

“…The cDNA was performed on Rotor-gene Q real-time PCR system with SYBR Green PCR Kit (Qiagen, Germantown, MD, USA) and transcript specific primers. The cDNAs have been checked expression of heat stress marker (GMHsp90C2.1) which can validate for the plants applied heat stress (Xu et al 2013 …”

Section: Validation Of Differentially Expressed Genes From Rna-seq Usmentioning

confidence: 99%

“…In soybean, heat stress significantly decreased plant growth and yield. Recently, mechanism of HSP20 and HSP90 families were studied to understand of heat response system in soybean (Lopes-Caitar et al 2013;Xu et al 2013). But there were not enough attempts to reveal the profiling of DEGs involved in HSP families under heat stress in soybean.…”

Section: Introductionmentioning

confidence: 99%

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Expression of Heat Shock Proteins by Heat Stress in Soybean

Song

Yim

Lee

2017

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Heat stress is one of the factors disturb productivity and growth of plants. Many genes including heat shock protein (HSP), heat shock transcription factors (HSF) and chaperones, were identified and characterized in many plants to play role in increased tolerance to abiotic stress. To reveal responsive gene to heat stress, we performed RNA-seq using two Korean soybean varieties under heat stress and normal conditions. The transcripts were analyzed, and we obtained 2,458 genes including 46 co-up regulation and 55 co-down regulated genes in both soybean varieties. We also revealed HSPs, HSFs and chaperones in the differentially expressed genes using BLAST and Pfam analyzation and verified expression changes under heat stress. Finally, we find 68 genes involved in HSP, HSF, chaperones in heat responsive genes associated increasing heat tolerance. As a result, relatively small HSP families were up regulated and continuously expressed in long period heat stress. On the other hand, large molecule HSPs, HSFs and chaperonin did not response to long heat stress. The expression profiling and characterization provide invaluable information to understand heat tolerance of soybean.

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“…However, the mechanism of chamomile's resilience to natural conditions is not well-understood. Several reports have indicated that Hsp90 is closely related to the stress resistance of plants (Xu et al, 2013). Whether there is functional connection between Hsp90 and the stress resistance in M. recutita remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita

Ling¹,

Su²,

Zhang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Heat shock protein 90 (Hsp90) is one of the most abundant and conserved chaperone proteins and plays important roles in plant growth and responses to environmental stimuli. However, little is known regarding the sequence and function of Hsp90s in Matricaria recutita. In the present study, we cloned the full-length cDNA sequence of the hsp90 gene from this species. Using rapid amplification of cDNA ends technologies with 2 degenerate primers that were designed based on the hsp90 gene sequence from other members of Asteraceae, we isolated and characterized an Hsp90 homolog gene from M. recutita (Mr-Hsp90). The full-length Mr-hsp90 cDNA sequence, containing 2097 base pairs, encodes a protein of 698 amino acids. Based on amino acid sequence identity, Mr-Hsp90 showed high similarity to other cloned Hsp90 proteins. The Mr-Hsp90 protein was closely clustered with the Lactuca sativa in a phylogenetic tree. These results indicate that the cloned sequence of Mr-Hsp90 is a member of the Hsp90 family, which is reported for the first time in M. recutita. Next, we conducted a salt stress experiment to determine the protein's function under salt stress conditions. Survival of chamomile seedlings subjected to heat-shock pretreatment was significantly increased compared with groups that had not undergone heat-shock pretreatment in a salt stress 10995 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 10994-11003 (2014) Cloning and characterization of environment. This indicates that Mr-Hsp90 plays an important role in the salt resistance of chamomile seedlings.

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Overexpression of GmHsp90s, a Heat Shock Protein 90 (Hsp90) Gene Family Cloning from Soybean, Decrease Damage of Abiotic Stresses in Arabidopsis thaliana

Cited by 96 publications

References 60 publications

The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava

The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava

Expression of Heat Shock Proteins by Heat Stress in Soybean

Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita

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