2020
DOI: 10.1186/s13068-020-01726-8
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Abstract: Background Oleaginous microalgae represent a valuable resource for the production of high-value molecules. Considering the importance of omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs) for human health and nutrition the yields of high-value eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) require significant improvement to meet demand; however, the current cost of production remains high. A promising approach is to metabolically engineer strains with enhanced levels of triacylglycerols (TAG… Show more

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Cited by 49 publications
(29 citation statements)
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“…Overall, the overexpression of PtDGATs in P. tricornutum exhibited slight effect on cell growth, which entered the stationary growth phase after 7 days of cultivation (Figure 5b). This is consistent with the phenomenon previously observed for algae overexpressing DGATs (Cui et al, 2018;Haslam et al, 2020;Klaitong et al, 2017;Niu et al, 2013;Wei et al, 2017). Nevertheless, some reports indicated that DGAT overexpression caused a significant decrease in algae growth (Dinamarca et al, 2017; Zienkiewicz et al, 2017;…”
Section: Ptdgat1 Overexpression Shows Little Effect On Growth Of Psupporting
confidence: 89%
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“…Overall, the overexpression of PtDGATs in P. tricornutum exhibited slight effect on cell growth, which entered the stationary growth phase after 7 days of cultivation (Figure 5b). This is consistent with the phenomenon previously observed for algae overexpressing DGATs (Cui et al, 2018;Haslam et al, 2020;Klaitong et al, 2017;Niu et al, 2013;Wei et al, 2017). Nevertheless, some reports indicated that DGAT overexpression caused a significant decrease in algae growth (Dinamarca et al, 2017; Zienkiewicz et al, 2017;…”
Section: Ptdgat1 Overexpression Shows Little Effect On Growth Of Psupporting
confidence: 89%
“…Our results demonstrated that PtDGAT1, likely localized at the chloroplast endoplasmic reticulum (ER), surpassed the other DGATs in synthesizing TAG. Moreover, overexpressing PtDGAT1 allowed P. tricornutum to produce more than doubled TAG, which reached 57.0% of dry weight, much higher than that achieved in the previously engineered P. tricornutum (Cui et al, 2018;Dinamarca et al, 2017;Haslam et al, 2020;Niu et al, 2013;Zulu et al, 2017). We also evaluated the performance of manipulating DGAT gene for TAG enhancement by P. tricornutum and discussed their biotechnological implications.…”
Section: Introductionmentioning
confidence: 95%
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“…Photosynthetic microalgae harbor the biosynthetic machinery to synthesize high-value biochemicals using carbon dioxide as the substrate, thereby emerging as the sustainable microbial cell factories and potentially offering promising solutions for contemporary commercial and environmental issues [1]. Particularly, triacylglycerides (TAGs) from oleaginous microalgae have been regarded as the most preferable feedstock for the production of biodiesel and a range of valuable oleochemicals [2].…”
Section: Introductionmentioning
confidence: 99%