Overexpression of a Xylanase Inhibitor Gene, OsHI-XIP, Enhances Resistance in Rice to Herbivores

Xin, Zhaojun; Wang, Qi; Yu, Zhiming; Hu, Liucheng; Li, Jiancai; CaiYu, Xiang; Wang, Baohui; Lou, Yonggen

doi:10.1007/s11105-013-0661-5

Cited by 23 publications

(20 citation statements)

References 46 publications

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“…Our results revealed that herbivore infestation, mechanical wounding and MeJA treatment enhanced the expression of OsXIP at the transcriptional and protein levels (Fig. 1), which is consistent with OsHI-XIP [5]. To date, there is one report that the xylanase inhibitor XIP-I could inhibit a xylanase from the digestive tract of the coffee berry borer [30].…”

Section: Discussionsupporting

confidence: 80%

Identification of two transcription factors activating the expression of OsXIP in rice defence response

Zhan

Sun

Rong³

et al. 2017

BMC Biotechnol

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BackgroundXylanase inhibitors have been confirmed to be involved in plant defence. OsXIP is a XIP-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown.ResultsHerbivore infestation, wounding and methyl jasmonate (MeJA) treatment enhanced mRNA levels and protein levels of OsXIP. By analyzing different 5’ deletion mutants of OsXIP promoter exposed to rice brown planthopper Nilaparvata lugens stress, a 562 bp region (–1451 – −889) was finally identified as the key sequence for the herbivores stress response. Using yeast one-hybrid screening, coupled with chromatin immunoprecipitation analysis, a basic helix-loop-helix protein (OsbHLH59) and an APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor OsERF71 directly binding to the 562 bp key sequence to activate the expression of OsXIP were identified, which is further supported by transient expression assay. Moreover, transcriptional analysis revealed that mechanical wounding and treatment with MeJA resulted in an obvious increase in transcript levels of OsbHLH59 and OsERF71 in root and shoot tissues.ConclusionsOur data shows that two proteins as direct transcriptional activators of OsXIP responding to stress were identified. These results reveal a coordinated regulatory mechanism of OsXIP, which may probably be involved in defence responses via a JA-mediated signaling pathway.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-017-0344-7) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 80%

Identification of two transcription factors activating the expression of OsXIP in rice defence response

Zhan

Sun

Rong³

et al. 2017

BMC Biotechnol

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“…Over-expression of the PAP1 transcription factor in tobacco, responsible for accumulation of anthocyanin pigments and other flavonoids/ phenylpropanoids exhibited greater resistance to the herbivore insect, Spodoptera litura (Mitsunami et al, 2014). Xin et al (2014) showed that over-expression of a xylanase inhibitor gene, OsHI-XIP decreased the feeding and oviposion preferences of the rice BPH, N . lugens .…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Rice OsLCB2a1 Gene and Functional Analysis of its Role in Insect Resistance

et al. 2016

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In plants, sphingolipids, such as long-chain bases (LCBs), act as bioactive molecules in stress responses. Until now, it is still not clear if these lipids are involved in biotic stress responses to herbivore. Herein we report that a rice LCB gene, OsLCB2a1 encoding a subunit of serine palmitoyltransferase (SPT), a key enzyme responsible for the de novo biosynthesis of sphingolipids, plays a critical role in plant defense response to the brown planthopper (BPH) attack and that its up-regulation protects plants from herbivore infestation. Transcripts of OsLCB2a1 gene in rice seedlings were increased at 4 h, but decreased at 8–24 h after BPH attack. Sphingolipid measurement profiling revealed that overexpression of OsLCB2a1 in Arabidopsis thaliana increased trihydroxylated LCB phytosphingosine (t18:0) and phytoceramide by 1.7 and 1.3-fold, respectively, compared with that of wild type (WT) plants. Transgenic Arabidopsis plants also showed higher callose and wax deposition in leaves than that of WT. Overexpression of OsLCB2a1 gene in A. thaliana reduced the population size of green peach aphid (Myzus persicae). Moreover, the electrical penetration graph (EPG) results indicated that the aphids encounter resistance factors while reaching for the phloem on the transgenic plants. The defense response genes related to salicylic acid signaling pathway, remained uplgulated in the OsLCB2a1-overexpressing transgenic plants. Our data highlight the key functions of OsLCB2a1 in biotic stress response in plants.

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“…The plant cell wall is mainly composed of non-starch polysaccharides, including cellulose and hemicellulose [35]. Cellulase (1,4,-β-glucanase) is the first enzyme reported to contribute to wall loosening during abscission [36].…”

Section: Cell Wall Hydrolysis Related Genesmentioning

confidence: 99%

“…Xylan is the major component of hemicelluloses. Xylanase can catalyze the hydrolysis of the β-1,4-xylosidic bonds in xylan, the activity of xylanase can be inhibited by xylanase inhibitors (XIs) [35]. Xylanases have been Fig.…”

Section: Cell Wall Hydrolysis Related Genesmentioning

confidence: 99%

“…4 Heat map diagram of the expression levels of 27 differentially expressed transcripts (DETs) involved hydrolase activity. The DETs were found between high seed shattering genotype XH09 and low seed shattering genotype ZhN03 at 21 days after heading reported to play an important role in plant defense against pathogens [43] and herbivores [35]. However, whether xylanases are also involved in seed shattering remains largely unknown.…”

Section: Cell Wall Hydrolysis Related Genesmentioning

confidence: 99%

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Transcriptome profiling of Elymus sibiricus, an important forage grass in Qinghai-Tibet plateau, reveals novel insights into candidate genes that potentially connected to seed shattering

et al. 2017

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Background: Elymus sibiricus is an important forage grass in semi-arid regions, but it is difficult to grow for commercial seed production due to high seed shattering. To better understand the underlying mechanism and explore the putative genes related to seed shattering, we conducted a combination of morphological, histological, physiochemical and transcriptome analysis on two E. sibiricus genotypes (XH09 and ZhN03) that have contrasting seed shattering.

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Overexpression of a Xylanase Inhibitor Gene, OsHI-XIP, Enhances Resistance in Rice to Herbivores

Cited by 23 publications

References 46 publications

Identification of two transcription factors activating the expression of OsXIP in rice defence response

Identification of two transcription factors activating the expression of OsXIP in rice defence response

Molecular Characterization of Rice OsLCB2a1 Gene and Functional Analysis of its Role in Insect Resistance

Transcriptome profiling of Elymus sibiricus, an important forage grass in Qinghai-Tibet plateau, reveals novel insights into candidate genes that potentially connected to seed shattering

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