Overcoming Fungal Multidrug Resistance by Natural Compounds Targeting Efflux Pumps

Singh, Shweta; Hans, Sandeep; Fatima, Zeeshan; Hameed, Saif

doi:10.2174/9781681085623118070008

Cited by 3 publications

(2 citation statements)

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“…The ability of the essential oils to penetrate and damage the intracellular membranes and cellular structure of fungi adds to the breakdown of mitochondrial cells. Singh et al(2017). 4) showed the effect of fennel and coriander volatile oils at 400 ppm on total volatile nitrogen of beef burger during cold storage at 4±1ºC for 16 days.…”

Section: Yeasts and Molds Counts (Log Cfu/g)mentioning

confidence: 99%

Vital Value of Coriander and Fennel Volatile Oils on Quality Beef Burger During Cryopreservation

Saleh

El-Desouky

Sharoba

et al. 2022

Journal of Food and Dairy Sciences

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Effect of coriander and fennel volatile oils on quality and shelf life of beef burger were investigated. GC/MS was used to chemical components of coriander and fennel. 2-Decenal (E) accounts for 34.69 percent of total chemical components in coriander volatile oil and Estragole (60.59 percent) in fennel volatile oil. DPPH was used to evaluate antioxidant properties of volatile oils derived from fennel and coriander. Two essential oils were tested in this experiment. Fennel and coriander essential oils were examined for their antimicrobial properties against two mould strains and four bacterial strains that are most commonly found in meat and meat products. The two essential oils' inhibitory effects could be arranged as follows: Fennel > coriander essential oils. Obtained essential oils could be used in industrial food as organic antioxidants and antimicrobials. Beef burger was added with 400 ppm coriander and fennel volatile oil. Microbial examination such as total viable count, psychrotrophic counts, lipolytic and proteolytic bacteria counts, coliform group bacteria and yeasts and moulds counts and chemical evaluation namely thiobarbituric acid, total volatile basic nitrogen, and pH value at 4 day intervals throughout 16 day. Beef burger control treatments had least microbial quality and freshness properties loss during the storage period.

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Section: Yeasts and Molds Counts (Log Cfu/g)mentioning

confidence: 99%

Vital Value of Coriander and Fennel Volatile Oils on Quality Beef Burger During Cryopreservation

Saleh

El-Desouky

Sharoba

et al. 2022

Journal of Food and Dairy Sciences

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“…Plants are a great resource for the discovery of compounds with pharmacological potential [23][24][25][26][27][28][29], with some plant extracts or extract-derived components belonging to different chemical families, targeting Mdr1 or Cdr1 [30][31][32]. The immense chemical diversity of secondary metabolites from plants and the low number of species explored to date [33], encourage the scienti c community and the pharmaceutical industry to include these products in e ux pump inhibitor (EPI) discovery pipelines.…”

Section: Introductionmentioning

confidence: 99%

Extracts from Argentinian native plants reverse fluconazole resistance in Candida species by inhibiting the efflux transporters Mdr1 and Cdr1

Gil

Laiolo

Bayona-Pacheco³

et al. 2022

Preprint

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Background: The development of multidrug resistance (MDR) associated with the overexpression of the efflux transporters Mdr1 and Cdr1 in Candida species impedes antifungal therapies. The urgent need for novel agents able to inhibit the function of both pumps, led us to evaluate this property in 137 extracts obtained from Argentinian plants. Methods: The ability of the extracts to reverse efflux pump-mediated MDR was determined with an agar chemosensitization assay using fluconazole (FCZ) resistant Mdr1- and Cdr1-overexpressing clinical isolates of Candida albicans and Candida glabrata as well as Saccharomyces cerevisiae strains selectively expressing Mdr1 (AD/CaMDR1) or Cdr1 (AD/CaCDR1). The resistance-reversing activity of the most potent extracts was further confirmed using a Nile Red accumulation assay. Results: Fifteen plant extracts overcame the FCZ resistance of Candida albicans 1114, which overexpresses CaMdr1 and CaCdr1, and AD/CaMDR1, with those from Acalypha communis and Solanum atriplicifolium being the most effective showing 4- to 16-fold reversal of resistance at concentrations ≥ 25 µg/mL. Both extracts, and to a lesser extent that from Pterocaulon alopecuroides, also restored FCZ sensitivity in CgCdr1-overexpressing C. glabrata 109 and in AD/CaCDR1 with fold reversal values ranging from 4 to 32 and therefore demonstrating a dual effect against Mdr1 and Cdr1. Both, A. communis and S. atriplicifolium extracts at concentrations ≥ 12.5 and ≥ 25 µg/mL, respectively, increased the intracellular Nile Red accumulation in all yeast strains overexpressing efflux pumps. Conclusions: The non-toxic and highly active extracts from A. communis and S. atripicifolium, provide promising sources of compounds for potentiating the antifungal effect of FCZ by blocking the efflux function of Mdr1 and Cdr1 transporters.

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A State-of-the-art Review and Prospective Therapeutic Applications of Prenyl Flavonoids as Chemosensitizers against Antifungal Multidrug Resistance in Candida albicans

Santi

Ortega

Andrea

2022

CMC

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Multidrug resistance (MDR) in the opportunistic pathogen Candida albicans is defined as non-susceptibility to at least one agent in two or more drug classes. This phenomenon has been increasingly reported since the rise in the incidence of fungal infections in immunocompromised patients at the end of the last century. After the discovery of efflux pump overexpression as a principal mechanism causing MDR in Candida strains, drug discovery targeting fungal efflux transporters has had a growing impact. Chemosensitization aims to enhance azole intracellular concentrations through combination therapy with transporter inhibitors. Consequently, the use of drug efflux inhibitors combined with the antifungal agent will sensitize the pathogen. As a result, the use of lower drug concentrations will reduce possible adverse effects on the host. Through an extensive revision of the literature, this review aims to provide an exhaustive and critical analysis of the studies carried out in the past two decades, regarding the chemosensitization strategy to cope with multidrug resistance in C. albicans. This work provides a deep analysis of the research about the inhibition of drug-efflux membrane transporters by prenylated flavonoids and the interactions of these phytocompounds with azole antifungals as an approach to chemosensitize multidrug-resistant C. albicans strains. We highlight the importance of prenylflavonoids and their particular chemical and pharmacological characteristics that make them excellent candidates with therapeutic potential as chemosensitizers. Finally, we propose the need for further research of prenyl flavonoids as inhibitors of drug-efflux mediated fungal resistance.

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Overcoming Fungal Multidrug Resistance by Natural Compounds Targeting Efflux Pumps

Cited by 3 publications

References 0 publications

Vital Value of Coriander and Fennel Volatile Oils on Quality Beef Burger During Cryopreservation

Vital Value of Coriander and Fennel Volatile Oils on Quality Beef Burger During Cryopreservation

Extracts from Argentinian native plants reverse fluconazole resistance in Candida species by inhibiting the efflux transporters Mdr1 and Cdr1

A State-of-the-art Review and Prospective Therapeutic Applications of Prenyl Flavonoids as Chemosensitizers against Antifungal Multidrug Resistance in Candida albicans

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