Over-expression of TaEXPB23, a wheat expansin gene, improves oxidative stress tolerance in transgenic tobacco plants

Han, Yangyang; Chen, Yanhui; Yin, Suhong; Zhang, Meng; Wang, Wei

doi:10.1016/j.jplph.2014.09.007

Cited by 69 publications

(40 citation statements)

References 43 publications

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“…These results suggest that, regardless of the mechanism of resistance displayed, the AraEXLB8 gene might have a more downstream role in the pathogen triggering response (PTI), and reinforces the need for a further understanding of molecular, biochemical and cellular events that culminate in pathogen resistance. The notable induction of AraEXLB8 in A. stenosperma leaves in response to UV can be also associated with the general ROS-scavenging and signal transduction pathways that triggered changes in the expression of some genes, as expansins, and in the production of secondary metabolites, as resveratrol, to control numerous biological process, including pathogen defense (Baxter et al 2014; Han et al 2015; Lopes et al 2013), and therefore, contributing to the HR resistance mechanism of A. stenosperma , as previously suggested (Guimaraes et al 2010; Morgante et al 2013). UV irradiation and other physical stresses induce ‘cross-tolerance’ responses that are commonly associated with pathogen defense and reactions to wounding, promoting increased resistance to pathogens (Mintoff et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, the reduction of expansin transcripts, which inhibits cell expansion, leads to an increase in resistance to plant diseases possibly due to a more efficient cell wall physical protection (Ding et al 2008; Gal et al 2006). Expansins are also involved in responses to oxidative stress (Han et al 2015) derived from enhanced production and accumulation of Reactive Oxygen Species (ROS) (Baxter et al 2014). As a secondary stress, oxidative damage is generally ubiquitous in almost all types of stresses, including ultraviolet (UV) exposure, thus supporting the idea that expansins are common and crucial players in the response to multiple and simultaneous stresses.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene

et al. 2017

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Expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. The first genome-wide analysis of the expansin superfamily in the Arachis genus identified 40 members in A. duranensis and 44 in A. ipaënsis, the wild progenitors of cultivated peanut (A. hypogaea). These expansins were further characterized regarding their subfamily classification, distribution along the genomes, duplication events, molecular structure, and phylogeny. A RNA-seq expression analysis in different Arachis species showed that the majority of these expansins are modulated in response to diverse stresses such as water deficit, root-knot nematode (RKN) infection, and UV exposure, with an expansin-like B gene (AraEXLB8) displaying a highly distinct stress-responsive expression profile. Further analysis of the AraEXLB8 coding sequences showed high conservation across the Arachis genotypes, with eight haplotypes identified. The modulation of AraEXLB8 expression in response to the aforementioned stresses was confirmed by qRT-PCR analysis in distinct Arachis genotypes, whilst in situ hybridization revealed transcripts in different root tissues according to the stress imposed. The overexpression of AraEXLB8 in soybean (Glycine max) composite plants remarkably decreased the number of galls in transformed hairy roots inoculated with RKN. This study improves the current understanding of the molecular evolution, divergence, and gene expression of expansins in Arachis, and provides molecular and functional insights into the role of expansin-like B, the less-studied plant expansin subfamily.Electronic supplementary materialThe online version of this article (doi:10.1007/s11103-017-0594-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene

et al. 2017

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“…They are known to participate in cell wall dynamics by contributing to either loosening or stiffening of the cell wall components [37,50]. It was recently showed that the over-expression of a wheat expansin in tobacco plants strongly enhanced the peroxidase activity of a cell wall bound fraction, thus suggesting that there may be some relationship between class III Prxs and expansins during plant cell growth and in response to environmental stresses [51]. …”

Section: Discussionmentioning

confidence: 99%

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

et al. 2016

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Brachypodium distachyon is a suitable plant model for studying temperate cereal crops, such as wheat, barley or rice, and helpful in the study of the grain cell wall. Indeed, the most abundant hemicelluloses that are in the B. distachyon cell wall of grain are (1-3)(1-4)-β-glucans and arabinoxylans, in a ratio similar to those of cereals such as barley or oat. Conversely, these cell walls contain few pectins and xyloglucans. Cell walls play an important role in grain physiology. The modifications of cell wall polysaccharides that occur during grain development and filling are key in the determination of the size and weight of the cereal grains. The mechanisms required for cell wall assembly and remodelling are poorly understood, especially in cereals. To provide a better understanding of these processes, we purified the cell wall at three developmental stages of the B. distachyon grain. The proteins were then extracted, and a quantitative and comparative LC-MS/MS analysis was performed to investigate the protein profile changes during grain development. Over 466 cell wall proteins (CWPs) were identified and classified according to their predicted functions. This work highlights the different proteome profiles that we could relate to the main phases of grain development and to the reorganization of cell wall polysaccharides that occurs during these different developmental stages. These results provide a good springboard to pursue functional validation to better understand the role of CWPs in the assembly and remodelling of the grain cell wall of cereals.

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“…Several studies have shown that genes expressing expansins are induced by environmental stimuli and enhance plant tolerance to various abiotic stresses. These include genes such as ZmEXPA1, ZmEXPA2, ZmEXPA6, and ZmEXPA8 in maize [30] and TaEXPB23 in wheat [31], which respond to a water deficit. GmEXPLB1 in soybeans [32] respond to waterlogging.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Jadamba

Kang

Paek

et al. 2020

IJMS

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Expansins are key regulators of cell-wall extension and are also involved in the abiotic stress response. In this study, we evaluated the function of OsEXPA7 involved in salt stress tolerance. Phenotypic analysis showed that OsEXPA7 overexpression remarkably enhanced tolerance to salt stress. OsEXPA7 was highly expressed in the shoot apical meristem, root, and the leaf sheath. Promoter activity of OsEXPA7:GUS was mainly observed in vascular tissues of roots and leaves. Morphological analysis revealed structural alterations in the root and leaf vasculature of OsEXPA7 overexpressing (OX) lines. OsEXPA7 overexpression resulted in decreased sodium ion (Na+) and accumulated potassium ion (K+) in the leaves and roots. Under salt stress, higher antioxidant activity was also observed in the OsEXPA7-OX lines, as indicated by lower reactive oxygen species (ROS) accumulation and increased antioxidant activity, when compared with the wild-type (WT) plants. In addition, transcriptional analysis using RNA-seq and RT-PCR revealed that genes involved in cation exchange, auxin signaling, cell-wall modification, and transcription were differentially expressed between the OX and WT lines. Notably, salt overly sensitive 1, which is a sodium transporter, was highly upregulated in the OX lines. These results suggest that OsEXPA7 plays an important role in increasing salt stress tolerance by coordinating sodium transport, ROS scavenging, and cell-wall loosening.

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Over-expression of TaEXPB23, a wheat expansin gene, improves oxidative stress tolerance in transgenic tobacco plants

Cited by 69 publications

References 43 publications

Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene

Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

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